2012 Promega catalogue

More documents

Recommendations

Info

Life Science Catalog 2012 Worldwide Contact List Section Contents Table of Contents Cell Signaling Protein Labeling and Detection 234 HaloTag ® Fluorescent Ligands Product Size Conc. Cat.# Price ($) HaloTag ® TMR Ligand 30 µl 5 mM G8251 904.00 15 µl 5 mM G8252 504.00 HaloTag ® Oregon Green ® Ligand 30 µl 1 mM G2801 904.00 15 µl 1 mM G2802 504.00 HaloTag ® diAcFAM Ligand 30 µl 1 mM G8272 904.00 15 µl 1 mM G8273 504.00 HaloTag ® Coumarin Ligand 30 µl 10 mM G8581 904.00 15 µl 10 mM G8582 504.00 HaloTag ® Alexa Fluor ® 488 Ligand 30 µl 1 mM G1001 904.00 15 µl 1 mM G1002 504.00 HaloTag ® TMRDirect Ligand 30 µl 0.1 mM G2991 521.00 HaloTag ® R110Direct Ligand 30 µl 0.1 mM G3221 521.00 HaloTag ® Biotin Ligand 30 µl 5 mM G8281 904.00 15 µl 5 mM G8282 504.00 HaloTag ® PEG-Biotin Ligand 30 µl 5 mM G8591 904.00 For Research Use Only. Not for Use in Diagnostic Procedures. 15 µl 5 mM G8592 504.00 Description: The HaloTag ® Fluorescent Ligands can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag ® Fluorescent Ligands allow researchers to apply the chloroalkane group that HaloTag ® protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications. HaloTag ® Fluorescent Ligands for Cellular Imaging Cell-permeant fluorescent ligands (rapid labeling protocol): • HaloTag ® TMR Ligand (555 Ex/585 Em) • HaloTag ® Oregon Green ® Ligand (496 Ex/516 Em) • HaloTag ® diAcFAM Ligand (494 Ex/526 Em) • HaloTag ® Coumarin Ligand (353 Ex/434 Em) Cell-impermeant fluorescent ligands for cell-surface labeling (rapid labeling protocol): • HaloTag ® Alexa Fluor ® 488 Ligand (494 Ex/517 Em) Cell-permeant fluorescent ligands (“no wash” protocol): • HaloTag ® TMRDirect Ligand (555 Ex/585 Em) • HaloTag ® R110Direct Ligand (502 Ex/527 Em) The Alexa Fluor ® 488 Ligand is impermeable to cell membranes and, therefore, used to label cell surface proteins. The TMR Ligand, Oregon Green ® Ligand, diAcFAM Ligand and Coumarin Ligand readily cross the cell membrane and, therefore, can be used to label intracellular proteins. HaloTag ® Ligands for Protein Detection The HaloTag ® Biotin Ligand consists of a 12-atom linker arm to biotin and is used as an affinity tag to capture the HaloTag ® protein-based fusion construct using the strong biotin-streptavidin interaction. The HaloTag ® PEG-Biotin Ligand contains a spacer not found in the HaloTag ® Biotin Ligand. This provides a significantly longer and more flexible linker between streptavidin and the HaloTag ® protein, which may be advantageous in preserving the activity of a HaloTag ® fusion partner protein upon immobilization or derivatization. Features: • Label in Solution or on a Solid Support: The HaloTag ® Ligands bind to the HaloTag ® protein or protein fusions with high specificity and affinity. • Label Your HaloTag ® Protein in Live Cells: The HaloTag ® TMR, diAcFAM, Coumarin and Biotin Ligands readily cross the cell membrane. • Pull Down Protein Complexes: The spacer and reactive linker of the HaloTag ® PEG-Biotin Ligand provide ideal pull-down capabilities. Alternatively, pull down directly with the HaloLink Resin. • Image Fixed Cells: The covalent bond is stable, allowing imaging of fixed cells and analysis of the labeled protein under stringent conditions. • Introduce Novel Functionalities or Perform Sequential Labeling: The open architecture of the technology enables the use of different ligands for multiple applications. • Design Only One Genetic Construct for Multiple Experiments: Obtain new functionality by using a different HaloTag ® Ligand without having to design and clone a new expression construct. Protocol Part# HaloTag ® Technology: Focus on Imaging Technical Manual TM260 HaloTag ® Biotin Ligand Protocol 9PIG828 HaloTag ® PEG-Biotin Ligand Protocol 9PIG859 A. C. D. Colabeling of HaloTag ® -p65 fusion protein with HaloTag ® TMR Ligand and the Anti-HaloTag ® pAb. Panel A. Cytoplasmic (red) labeling of HEK293-p65-HT2 cells by HaloTag ® TMR Ligand. Panel B. Cytoplasmic (green) labeling by Anti-HaloTag ® pAb and Alexa Fluor ® 488-conjugated antirabbit IgG (Invitrogen). Panel C. Colocalization of ligand and antibody binding activities. Panel D. Merger of red and green fluorescence with counterstaining of the nucleus by DAPI (blue). Arrows denote rare cells that show little or no expression of HaloTag ® -p65. Protocols developed and performed at Promega. For complete and up-to-date product information visit: www.promega.com/catalog B. 6312TB
Cell Signaling HaloTag ® Ligand Building Blocks Product Size Cat.# Price ($) HaloTag ® Amine (O4) Ligand 5 mg P6741 909.00 HaloTag ® Amine (O2) Ligand 5 mg P6711 909.00 HaloTag ® Iodoacetamide (O4) Ligand 5 mg P6771 904.00 HaloTag ® Iodoacetamide (O2) Ligand 5 mg P1681 904.00 HaloTag ® Succinimidyl Ester (O4) Ligand 5 mg P6751 904.00 HaloTag ® Succinimidyl Ester (O2) Ligand 5 mg P1691 904.00 HaloTag ® Thiol (O4) Ligand 5 mg P6761 904.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The HaloTag ® Ligand Building Blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag ® Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag ® protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications. The HaloTag ® Succinimidyl Ester (04) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkyl chloride separated by three ethylene glycol repeats (04). The HaloTag ® Succinimidyl Ester (04) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag ® protein for any application of interest. The HaloTag ® Succinimidyl Ester (O2) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkylchloride separated by an ethylene glycol repeat (O2). The HaloTag ® Succinimidyl Ester (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag ® protein for any application of interest. The HaloTag ® Amine (04) Ligand contains a reactive amine group connected to an alkyl chloride, separated by an ethylene glycol repeat (04). The HaloTag ® Amine (04) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides, and TFP esters. The ligand with functional group can then be used with the HaloTag ® protein for any application of interest. The HaloTag ® Amine (O2) Ligand contains a reactive amine group connected to an alkylchloride, separated by an ethylene glycol repeat (O2). The HaloTag ® Amine (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide, or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides, and TFP esters. The ligand with functional group can then be used with the HaloTag ® protein for any application of interest. The HaloTag ® Iodoacetamide (04) Ligand contains a reactive iodoacetamide group connected an alkyl chloride separated by an ethylene glycol repeat (04). The HaloTag ® Iodoacetamide (04) Ligand has been designed to rapidly react with sulfhydryl-containing molecules, whether small organic compounds, peptides or proteins. The ligand with functional group can then be used with the HaloTag ® protein for any application of interest. For complete and up-to-date product information visit: www.promega.com/catalog The HaloTag ® Iodoacetamide (O2) Ligand contains a reactive iodoacetamide group connected to an alkylchloride separated by an ethylene glycol repeat (O2). HaloTag ® Iodoacetamide (O2) Ligand has been designed to rapidly react with sulfhydryl-containing molecules, whether small organic compounds, peptides or proteins. The ligand with functional group can then be used with the HaloTag ® protein for any application of interest. The HaloTag ® Thiol (O4) Ligand contains a reactive sulfhydryl group connected to an alkyl chloride separated by three ethylene glycol repeats (04). The HaloTag ® Thiol (04) Ligand can be successfully conjugated to any reporter group, cross-linking reagent (bound or free), or nucleic acid derivative containing a number of different alkylating groups, forming stable thioether bonds. Commonly used reagents that rapidly react with sulfhydryls include iodo- or bromo-acetyls or benzyls, bromo- or chloro-mustards, maleimides, aziridines, acryloyl derivatives, and halide or sulfonate containing arenes (those bearing Electron Withdrawing Groups (EWGs) react most rapidly). The reactive ligand can be captured with HaloTag ® protein either before or after the thiol group is functionalized for any application of interest. Storage Conditions: Store Cat.# P1691 and P6751 at or below –70°C under inert atmosphere. Store Cat.# P6711 and P6741 at or below –20°C in an air-tight container in the absence of light. Store Cat.# P1681, P6771 and P6761 at or below –20°C under inert atmosphere in the absence of light. See Promega Product Information for additional details on individual products. HaloTag ® Fusion (C-Terminal) Mammalian Expression Vectors Product Size Cat.# Price ($) pHTC HaloTag ® CMV-neo Vector 20 µg G7711 355.00 pFC27A HaloTag ® CMV-neo Flexi ® Vector 20 µg G8421 397.00 pFC27K HaloTag ® CMV-neo Flexi ® Vector 20 µg G8431 397.00 pFC14A HaloTag ® CMV Flexi ® Vector 20 µg G9651 397.00 pFC14K HaloTag ® CMV Flexi ® Vector 20 µg G9661 397.00 pFC15A HaloTag ® CMVd1 Flexi ® Vector 20 µg G1611 397.00 pFC15K HaloTag ® CMVd1 Flexi ® Vector 20 µg G1601 397.00 pFC16A HaloTag ® CMVd2 Flexi ® Vector 20 µg G1591 397.00 pFC16K HaloTag ® CMVd2 Flexi ® Vector 20 µg G1571 397.00 pFC17A HaloTag ® CMVd3 Flexi ® Vector 20 µg G1551 397.00 pFC17K HaloTag ® CMVd3 Flexi ® Vector 20 µg G1321 397.00 Available Separately HaloTag ® Cloning Starter System 1 each G6050 568.00 HaloTag ® Flexi ® Vectors—CMV Deletion Series Sample Pack For Research Use Only. Not for Use in Diagnostic Procedures. 9 × 2 µg G3780 333.00 235 13 Protein Expression and Analysis Section Contents Table of Contents
Page 1 and 2:
Life Science CATALOG 2012
Page 3 and 4:
Cell Signaling Table of Contents Pr
Page 5 and 6:
Cell Signaling For complete and up-
Page 7 and 8:
Cell Signaling Agarose, LMP, Prepar
Page 9 and 10:
Cell Signaling Blue/Orange Loading
Page 11 and 12:
Cell Signaling Glycine, Molecular B
Page 13 and 14:
Cell Signaling Sephacryl ® S-400 P
Page 15 and 16:
Cell Signaling Tris Base, Molecular
Page 17 and 18:
Cell Signaling Unmethylated Lambda
Page 19 and 20:
Cell Signaling Promega Barrier Tips
Page 21 and 22:
Cell Signaling Product Size Cat.# P
Page 23 and 24:
Cell Signaling 2 Cell Health Assays
Page 25 and 26:
Cell Signaling A. B. Luminescence/p
Page 27 and 28:
Cell Signaling Luminogenic Enzyme S
Page 29 and 30:
Cell Signaling Apoptosis
Page 31 and 32:
Cell Signaling Caspase-Glo ® 2 Ass
Page 33 and 34:
Cell Signaling Caspase-Glo ® 9 Ass
Page 35 and 36:
Cell Signaling CaspACE Assay System
Page 37 and 38:
Cell Signaling Anti-ACTIVE ® Caspa
Page 39 and 40:
Cell Signaling BacTiter-Glo Microbi
Page 41 and 42:
Cell Signaling CellTiter 96 ® Cell
Page 43 and 44:
Cell Signaling MultiTox-Fluor Multi
Page 45 and 46:
Cell Signaling CytoTox-Glo Cytotoxi
Page 47 and 48:
Cell Signaling CytoTox 96 ® Non-Ra
Page 49 and 50:
Cell Signaling GSH-Glo Glutathione
Page 51 and 52:
Cell Signaling 3 Cell Signaling For
Page 53 and 54:
Cell Signaling cAMP-Glo Max Assay P
Page 55 and 56:
Cell Signaling Growth Factors Epide
Page 57 and 58:
Cell Signaling SIRT-Glo Assays and
Page 59 and 60:
Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62:
Cell Signaling Kinase Enzyme System
Page 63 and 64:
Cell Signaling Kinase Enzyme System
Page 65 and 66:
Cell Signaling Kinase-Glo ® Kinase
Page 67 and 68:
Cell Signaling SignaTECT ® Protein
Page 69 and 70:
Cell Signaling Casein Kinase I Prod
Page 71 and 72:
Cell Signaling Product Size Cat.# P
Page 73 and 74:
Cell Signaling Protein Kinase Subst
Page 75 and 76:
Page 77 and 78:
Cell Signaling DNA Step Ladders Pro
Page 79 and 80:
Cell Signaling Conventional DNA Mar
Page 81 and 82:
Cell Signaling ProMega-Markers ® L
Page 83 and 84:
Cell Signaling Restriction Enzymes
Page 85 and 86:
Cell Signaling BamHI Product Size C
Page 87 and 88:
Cell Signaling EcoRI Product Size C
Page 89 and 90:
Cell Signaling NciI Product Size Co
Page 91 and 92:
Cell Signaling SpeI Product Size Co
Page 93 and 94:
Cell Signaling TSAP Thermosensitive
Page 95 and 96:
Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98:
Cell Signaling Kinases T4 Polynucle
Page 99 and 100:
Cell Signaling S1 Nuclease Product
Page 101 and 102:
Cell Signaling Percent Inhibition o
Page 103 and 104:
Cell Signaling Untagged Flexi ® Ma
Page 105 and 106:
Cell Signaling pGEM ® -5Zf(+) Vect
Page 107 and 108:
Cell Signaling pSP64 Poly(A) Vector
Page 109 and 110:
Page 111 and 112:
Cell Signaling x-tracta Gel Extract
Page 113 and 114:
Cell Signaling Wizard ® MagneSil
Page 115 and 116:
Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118:
Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120:
Cell Signaling MagneSil ® KF, Geno
Page 121 and 122:
Cell Signaling Plasmid Purification
Page 123 and 124:
Cell Signaling PureYield Plasmid Ma
Page 125 and 126:
Cell Signaling Wizard ® Plus Maxip
Page 127 and 128:
Cell Signaling Wizard ® MagneSil
Page 129 and 130:
Cell Signaling PureYield RNA Midipr
Page 131 and 132:
Cell Signaling Maxwell ® 16 System
Page 133 and 134:
Cell Signaling RNasin ® Plus RNase
Page 135 and 136:
Cell Signaling DNA Quantitation Qua
Page 137 and 138:
Cell Signaling Magnetic Stands and
Page 139 and 140:
Cell Signaling 6 Drug Discovery For
Page 141 and 142:
Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144:
Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146:
Cell Signaling Protease Assays Prot
Page 147 and 148:
Cell Signaling Cell-Based Proteasom
Page 149 and 150:
Cell Signaling Tryptase, Human, Rec
Page 151 and 152:
Cell Signaling 7 Genetic Identity F
Page 153 and 154:
Cell Signaling Maxwell ® 16 Forens
Page 155 and 156:
Cell Signaling Plexor ® HY System
Page 157 and 158:
Cell Signaling PowerPlex ® 18D Sys
Page 159 and 160:
Cell Signaling PowerPlex ® CS7 Sys
Page 161 and 162:
Cell Signaling PowerPlex ® 16 and
Page 163 and 164:
Cell Signaling GenePrint ® Fluores
Page 165 and 166:
Page 167 and 168:
Cell Signaling HaloTag ® Ligand Bu
Page 169 and 170:
Cell Signaling Block & Sample 5X Bu
Page 171 and 172:
Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174:
Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176:
Cell Signaling Anti-Human GDNF pAb
Page 177 and 178:
Cell Signaling Anti-PARP p85 Fragme
Page 179 and 180:
Cell Signaling Anti-VAChT pAb Produ
Page 181 and 182:
Cell Signaling VivoGlo Caspase 3/7
Page 183 and 184:
Page 185 and 186:
Cell Signaling Microbial Detection
Page 187 and 188: 10 Instruments For complete and up-
Page 189 and 190: GloMax ® 20/20 Luminometer Product
Page 191 and 192: Fluorescence Module: Application-op
Page 193 and 194: GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196: Maxwell ® 16 Instruments Maxwell
Page 197 and 198: Maxwell ® 16 Flexi Method Firmware
Page 199 and 200: Cell Signaling For complete and up-
Page 201 and 202: Cell Signaling Maxwell ® 16 System
Page 203 and 204: Cell Signaling Maxwell ® 16 Flexi
Page 205 and 206: Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR For complete
Page 209 and 210: Cell Signaling TaqBead Hot Start Po
Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
Page 219 and 220: Cell Signaling Reverse Transcriptio
Page 221 and 222: Cell Signaling AMV Reverse Transcri
Page 223 and 224: Cell Signaling PCR Cloning pGEM ®
Page 227 and 228: Cell Signaling TnT ® Quick Coupled
Page 229 and 230: Cell Signaling TnT ® T7 Quick for
Page 231 and 232: Cell Signaling Canine Pancreatic Mi
Page 233 and 234: Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236: Cell Signaling Immobilized Trypsin
Page 237: Cell Signaling Asp-N, Sequencing Gr
Page 241 and 242: Cell Signaling Inducible T7-Driven
Page 243 and 244: Cell Signaling ProtoBlot ® II AP S
Page 247 and 248: Cell Signaling HaloTag ® Protein P
Page 249 and 250: Cell Signaling HaloTag ® Mammalian
Page 251 and 252: Cell Signaling HaloLink Protein Arr
Page 253 and 254: Cell Signaling HaloCHIP System Prod
Page 255 and 256: Cell Signaling MagneHis Protein Pur
Page 257 and 258: Cell Signaling Streptavidin Product
Page 259 and 260: Cell Signaling Protein Purification
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274: Cell Signaling Promoter-Driven Cont
Page 275 and 276: Cell Signaling Nuclear Receptor Pat
Page 277 and 278: Cell Signaling pRL Renilla Lucifera
Page 279 and 280: Cell Signaling GloResponse Lucifera
Page 281 and 282: Cell Signaling pSP-luc+NF Fusion Ve
Page 283 and 284: Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286: Cell Signaling Transfection Reagent
Page 287 and 288: Cell Signaling 16 RNA Analysis For
Page 289 and 290:
Cell Signaling Riboprobe ® System
Page 291 and 292:
Cell Signaling HeLaScribe ® Nuclea
Page 293 and 294:
Cell Signaling RNA Interference Gen
Page 295 and 296:
Cell Signaling 17 Stem Cell Researc
Page 297 and 298:
Cell Signaling Cell ID System Produ
Page 299 and 300:
Cell Signaling StemElite Human Panc
Page 301 and 302:
Cell Signaling 18 Vectors For compl
Page 303 and 304:
Cell Signaling HaloTag ® Fusion (N
Page 305 and 306:
Cell Signaling pCI Mammalian Expres
Page 307 and 308:
Cell Signaling Reporter Vectors pGL
Page 309 and 310:
Cell Signaling pGEM ® -luc DNA Pro
Page 311 and 312:
Cell Signaling pGEM ® and pSP Subc
Page 313 and 314:
Cell Signaling pUC/M13 Sequencing P
Page 315 and 316:
Index: A-Z 19 Index For complete an
Page 317 and 318:
BssHII.............................
Page 319 and 320:
In vitro Transcription Systems Rela
Page 321 and 322:
QuantiFluor dsDNA System ..........
Page 323 and 324:
Cat# Product Size Page Price($) A10
Page 325 and 326:
Index by Catalog Number Cat# Produc
Page 327 and 328:
Cat# Product Size Page Price($) C84
Page 329 and 330:
Cat# Product Size Page Price($) E13
Page 331 and 332:
Cat# Product Size Page Price($) E61
Page 333 and 334:
Cat# Product Size Page Price($) G13
Page 335 and 336:
Cat# Product Size Page Price($) G77
Page 337 and 338:
Cat# Product Size Page Price($) L50
Page 339 and 340:
Cat# Product Size Page Price($) P16
Page 341 and 342:
Cat# Product Size Page Price($) R99
Page 343 and 344:
Cat# Product Size Page Price($) V42
Page 345 and 346:
Cat# Product Size Page Price($) W39
Page 347 and 348:
personal DETECTION GloMax Integrat
show all

2012 Promega catalogue

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?