2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
226
Wheat Germ Extract
Product Size Cat.# Price ($)
Wheat Germ Extract 5 × 200 µl L4380 260.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Wheat Germ Extract contains the cellular components necessary
for protein synthesis (tRNA, ribosomes, initiation, elongation and termination
factors). Wheat Germ Extract is prepared by grinding wheat germ in an extraction
buffer followed by centrifugation to remove cell debris. The supernatant is
subjected to chromatography that separates endogenous amino acids and
plant pigments from the extract. The extract is also treated with micrococcal
nuclease to destroy endogenous mRNA and thus reduce background translation
to a minimum.
Features:
• Optimized: Extract contains an energy-regenerating system (phosphocreatine/phosphocreatine
kinase), spermidine (to stimulate the efficiency of
chain elongation), magnesium acetate and potassium acetate.
• Flexible: Three Amino Acid Mixtures are provided, which enable different
choices of radioisotopes.
• Robust: Potassium Acetate is provided to enhance translation for a wide
range of mRNAs.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –70°C or below. Avoid freeze-thaw cycles.
Protocol Part#
Wheat Germ Extract Technical Manual TM230
T7 Sample System
Product Size Cat.# Price ($)
T7 Sample System
For Research Use Only. Not for Use in Diagnostic Procedures.
1 each L5900 241.00
Description: The T7 Sample System is designed to facilitate the optimization
of individual gene expression by offering four unique in vitro translation systems
to evaluate. The system consists of samples of: TnT ® T7 Quick for PCR DNA,
TnT ® T7 Quick Coupled Transcription/Translation System, TnT ® Coupled Wheat
Germ Extract System and E. coli T7 S30 Extract System for Circular DNA.
All of the coupled systems utilize RNA generated by a T7 phage promoter.
Criteria such as post-translational modifications, ionic optimization and detection
methods (i.e., non-isotopic) should be considered when choosing an in vitro
system. In some cases only direct experimental results will confirm which
system is best for specific genes.
Features:
• Variety: Four major in vitro translation systems to evaluate.
• Value: No requirement for the purchase of several large expensive
systems.
• Reliability: Comprised of rigorously quality-controlled reagents to ensure
the highest level of transcription/translation.
• Optimization: Determine which system is best for individual genes.
Storage Conditions: Store at –70°C.
Protocol Part#
T7 Sample System Technical Bulletin TB293
Rabbit Reticulocyte Lysate, Untreated
Product Size Cat.# Price ($)
Rabbit Reticulocyte Lysate, Untreated
For Research Use Only. Not for Use in Diagnostic Procedures.
1 ml L4151 186.00
Description: Untreated Rabbit Reticulocyte Lysate contains the cellular
components necessary for protein synthesis (tRNA, ribosomes, amino acids,
initiation, elongation and termination factors) but has not been treated with
micrococcal nuclease. Untreated Lysate is used primarily for the isolation
of these components and as an abundant source of endogenous globin
mRNA. Untreated Lysate is prepared from New Zealand white rabbits in the
same manner as treated lysates with the exception that it is not treated with
micrococcal nuclease.
Features:
• Reliable: Consistent reticulocyte production in each lot.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –70°C or below.
Protocol Part#
Rabbit Reticulocyte Lysate Technical Manual TM232
Luciferase Control RNA
Product Size Conc. Cat.# Price ($)
Luciferase Control RNA 20 µg 1 mg/ml L4561 193.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Luciferase Control RNA is a unique functional control for in
vitro translation reactions. Luciferase Control RNA is an uncapped in vitrotranscribed
RNA containing a 30-base poly(A) tail that produces functional
luciferase when translated. Control reactions are monitored easily by a luciferase
assay for the production of luminescence generated from the full-length
luciferase.
Features:
• Convenient: Control reactions are easily monitored by a luciferase assay
for luminescence.
• Safe: Non-radioactive format to monitor control activity.
Storage Conditions: Store at –70°C.
Luciferase SP6/T7 Control DNAs
Product Size Cat.# Price ($)
Luciferase SP6 Control DNA 20 µg L4741 193.00
Luciferase T7 Control DNA
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg L4821 193.00
Description: The Luciferase SP6 and T7 Control DNAs are used as functional
controls in the TnT ® Quick Coupled and TnT ® Coupled Transcription/Translation
Systems. The Control DNAs contain the gene for luciferase under transcriptional
control of a phage RNA polymerase promoter. All constructs carry a 30-base
pair poly[d(A)/d(T)] tail following the luciferase gene. Control reactions are
monitored easily by the production of luminescence, which is generated from
full-length luciferase and the addition of necessary components. Luciferase
Control DNAs are supplied as 0.5mg/ml solutions in TE buffer.
Storage Conditions: Store at –20°C.
For complete and up-to-date product information visit: www.promega.com/catalog