2012 Promega catalogue

Cell Signaling
TnT ® T7 Quick for PCR DNA
Product Size Cat.# Price ($)
TnT ® T7 Quick for PCR DNA 40 reactions L5540 914.00
For Laboratory Use.
Description: TnT ® T7 Quick for PCR DNA is a rapid, convenient, coupled
transcription/translation system designed for optimum protein expression from
PCR templates. For most PCR templates, the TnT ® T7 Quick for PCR DNA reactions
produce up to 5 times more protein than other commercially available kits.
The PCR-generated DNA can be used directly from the amplification reaction or
purified by numerous commercially available kits and traditional methods.
Features:
• Convenient: Directly from PCR, no cleanup necessary.
• High Yield: Up to 5 times more expressed protein than standard translation
reactions with linear templates.
• Quick: One-tube reaction.
• Complete: Reagents including Recombinant RNasin ® Ribonuclease Inhibitor
are included in the Quick Master Mix.
• Good Value: One-tube format means no leftover reagents.
• Reliable: The TnT ® Systems are rigorously quality controlled to ensure the
highest level of transcription/translation.
Storage Conditions: Store at –70°C. Do not freeze-thaw the Master Mix
more than two times.
Protocol Part#
TnT ® T7 Quick for PCR DNA Technical Manual
TM235
Rabbit Reticulocyte Lysate System, Nuclease
Treated
Product
Rabbit Reticulocyte Lysate System, Nuclease
Size Cat.# Price ($)
Treated 30 reactions L4960 260.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Rabbit Reticulocyte Lysate Translation Systems are utilized in the
identification of mRNA species, the characterization of their protein products
and the investigation of transcriptional and translational control. Rabbit
Reticulocyte Lysate is prepared from New Zealand white rabbits using a
standard protocol that ensures reliable and consistent reticulocyte production
in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal
nuclease to destroy endogenous mRNA and thus reduce background
translation to a minimum. The lysate contains the cellular components necessary
for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation
and termination factors).
Features:
• Consistent: Reliable and consistent translation with each lot.
• Optimized and Ready to Use: The treated Rabbit Reticulocyte Lysate
is optimized for translation and contains an energy-regenerating system
(phosphocreatine/phosphocreatine kinase), a mixture of tRNAs (to expand
the range of mRNAs that can be translated), hemin (to prevent inhibition of
initiation), and potassium chloride and magnesium acetate.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –70°C or below. Do not freeze-thaw the lysate
more than two times.
Protocol Part#
Rabbit Reticulocyte Lysate Technical Manual TM232
For complete and up-to-date product information visit: www.promega.com/catalog
Flexi ® Rabbit Reticulocyte Lysate System
Product Size Cat.# Price ($)
Flexi ® Rabbit Reticulocyte Lysate System 30 reactions L4540 391.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Flexi ® Rabbit Reticulocyte Lysate System allows translation
reactions to be optimized for a wide range of parameters, including Mg 2+ and
K + concentrations and the choice of adding DTT. To help optimize Mg 2+ for a
specific message, the endogenous Mg 2+ concentration of each lysate batch is
stated in the product information included with this product. The Flexi ® System
also offers the choice of three amino acid mixtures and includes a control RNA
encoding the firefly luciferase gene.
Features:
• Improved Efficiency: In an optimized system, the quantity of protein produced
can be increased up to fourfold over that of a standard lysate reaction.
• Easy Optimization: To aid in optimizing magnesium concentrations, the
endogenous magnesium concentration is provided for each lot of Flexi ®
Lysate.
• Choice: The Flexi ® System contains three Amino Acid Mixtures, which
enable different choices of radioisotopes.
• Control Included: Luciferase Control RNA and Luciferase Assay Reagent
are included with the system as a functional control. Only full-length
luciferase is active.
Storage Conditions: Store at –70°C, except Luciferase Assay Wells, which
can be stored at room temperature. Do not freeze-thaw the lysate more than
two times.
Protocol Part#
Flexi ® Rabbit Reticulocyte Lysate Systems Technical Bulletin TB127
Rabbit Reticulocyte Lysate/Wheat Germ
Extract Combination System
Product
Rabbit Reticulocyte Lysate/Wheat Germ
Size Cat.# Price ($)
Extract Combination System 24 reactions L4330 276.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination
System contains both Rabbit Reticulocyte Lysate and Wheat Germ Extract for
comparing in vitro translation systems. Reticulocyte Lysate is prepared from
New Zealand white rabbits. The Wheat Germ Extract is prepared by grinding
wheat germ in an extraction buffer followed by centrifugation to remove cellular
debris. Both systems contain the cellular components necessary for protein
synthesis. The systems have been treated with micrococcal nuclease, which
destroys endogenous mRNA and results in minimal background translation.
Features:
• Choice: Test both Rabbit Reticulocyte Lysate and Wheat Germ Systems to
find optimal translation systems.
• Consistent: Rigorous quality control ensures minimal lot-to-lot variability.
• Optimal Expression: Potassium Acetate is provided to enhance the
Wheat Germ Extract System for a wide range of mRNAs.
Storage Conditions: Store at –70°C or below. Do not freeze-thaw the lysate
more than two times.
Protocol Part#
Wheat Germ Extract Technical Manual TM230
225
13
Protein Expression and Analysis
Section
Contents
Table of
Contents