2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
224
TnT ® Coupled Wheat Germ Extract System
Product Size Cat.# Price ($)
TnT ® T3 Coupled Wheat Germ Extract System 40 reactions L4120 855.00
TnT ® SP6 Coupled Wheat Germ Extract
System 40 reactions L4130 855.00
TnT ® T7 Coupled Wheat Germ Extract System 40 reactions L4140 855.00
TnT ® T7/SP6 Coupled Wheat Germ Extract
System 40 reactions L5030 914.00
TnT ® T7/T3 Coupled Wheat Germ Extract
System 40 reactions L5040 914.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The TnT ® Coupled Wheat Germ Extract Systems offer researchers
an alternative for eukaryotic cell-free protein expression: a one-tube,
coupled transcription/translation system. The TnT ® Extract Systems greatly
simplify the process and reduce the time required to obtain in vitro translation
results. Standard wheat germ extract translations commonly use RNA
synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters. This entire
process requires separate reactions with several steps between each reaction.
The TnT ® Extracts bypass many of these steps by incorporating transcription
directly in the translation mix. Additionally, the TnT ® Extract reactions often
produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than
do standard in vitro wheat germ extract translations using RNA templates.
Magnesium Acetate, 25mM, and Potassium Chloride, 2.5M, can be used to
optimize in vitro translation reactions in the TnT ® T7 Quick Coupled Transcription/Translation
System, Flexi ® Rabbit Reticulocyte Lysate System and TnT ®
Coupled Wheat Germ Extract System.
Features:
• Reliable: The TnT ® Systems are rigorously quality controlled to ensure the
highest level of transcription/translation, whether your template is a linear
(T3 or T7 only) or circular plasmid.
• Convenient: Single-tube procedure eliminates the time and effort required
to prepare RNA for a standard wheat germ translation. Translation results
can be visualized by autoradiography in 6–8 hours.
• Versatile: The T3 system will produce protein from both linear and circular
DNA. Use only linear DNA with the T7 system. Use only circular DNA with
the SP6 system. For PCR templates use TnT ® T7 Quick for PCR DNA (Cat.#
L5540).
• Controls Included: Luciferase Control DNA and Luciferase Assay
Reagents are included with the system as functional controls. Only fulllength
luciferase is active.
Storage Conditions: Store the polymerase at –20°C. Store the Luciferase
Assay Wells at room temperature. Store the other components at –70°C. Avoid
multiple freeze-thaw cycles.
Protocol Part#
TnT ® Coupled Wheat Germ Extract Systems Technical Bulletin TB165
TnT ® Starter Bundle
Product Size Cat.# Price ($)
TnT ® T7 Quick Starter Bundle, Chemiluminescent 1 each L1210 1180.00
TnT ® T7 Quick Starter Bundle, Colorimetric 1 each L1215 1050.00
For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in
all countries. Please contact you local representative for more information.
Description: Get the tools you need in one bundle to start cell-free expression
and detection of your proteins of interest. Use the system for in vitro analysis of
protein:protein or protein:nucleic acid interactions, or to verify the ability of your
clone to express protein. Purchase this special bundle, and get the popular TnT ®
T7 Quick Coupled Transcription/Translation System, your choice of Transcend
Translation Detection System, and receive two cell-free expression-qualified
expression vectors, pTnT and pCMVTnT Vectors, at no extra cost.
Features:
• TnT ® T7 Quick Coupled Transcription/Translation System: Our most
popular cell-free translation system— a simple one-hour, one-tube reaction.
Requires only a protein coding sequence downstream of a T7 RNA
polymerase promoter to produce protein. Produced protein may be used
in a variety of applications including pull-downs, immunoprecipitations and
gel shift assays. TnT ® T7 Quick Coupled Transcription/Translation System
Technical Manual #TM045.
• Transcend Translation Detection Systems: A simple addition of
the Transcend biotin-labeled lysine tRNA to the TnT ® T7 Quick reaction
provides a simple means of tagging a protein for easy detection. Detect
proteins through simple Western blotting techniques with either chemiluminescent
or colorimetric techniques. Transcend Translation Detection
Systems Technical Bulletin #TB182.
• pTnT Vector: Specifically designed to work with the TnT ® Systems with
added features to enhance cell-free expression. pTnT Vector Technical
Bulletin #TB304.
• pCMVTnT Vector: Specifically designed to work with the TnT ® Systems
with added features to enhance cell-free expression. Go from cell-free expression
to mammalian expression directly with built-in CMV promoter. pC-
MVTnT Vector Technical Bulletin #TB305.
Storage Conditions: Store the TnT ® Quick System at –70°C. Do not freezethaw
the lysate more than two times. Store the Transcend tRNA at –70°C.
Do not subject the Transcend tRNA to more than five freeze-thaw cycles.
Store all other Transcend System components at 4°C. Store the pTnT and
pCMVTnT Vectors at –20°C.
For complete and up-to-date product information visit: www.promega.com/catalog