2012 Promega catalogue

Cell Signaling
TnT ® Quick Coupled Transcription/Translation
System
Product Size Conc. Cat.# Price ($)
TnT ® T7 Quick Coupled Transcription/
Translation System 40 reactions L1170 889.00
TnT ® T7 Quick Coupled Transcription/
Translation System, Trial Size 5 reactions L1171 239.00
TnT ® SP6 Quick Coupled
Transcription/Translation System 40 reactions L2080 889.00
TnT ® SP6 Quick Coupled
Transcription/Translation System,
Trial Size 5 reactions L2081 239.00
Magnesium Acetate 100 µl 25 mM L4581 76.00
Potassium Chloride 200 µl 2.5 M L4591 76.00
L4581, L4591 For Laboratory Use. L1170, L1171, L2080, L2081 For Research Use Only. Not for
Use in Diagnostic Procedures.
Description: The TnT ® Quick Systems are convenient single-tube, coupled
transcription/translation reactions for eukaryotic cell-free protein expression.
These cell-free expression systems combine the RNA Polymerase, nucleotides,
salts, amino acids and Recombinant RNasin ® Ribonuclease Inhibitor with the
reticulocyte lysate solution to form a single TnT ® Quick Master Mix.
The TnT ® Quick Coupled Transcription/Translation System is available in two
configurations for the expression of genes cloned downstream from either the
T7 or SP6 RNA polymerase promoters. To use these cell-free expression systems,
0.2–2.0μg of circular plasmid DNA containing a T7 or SP6 promoter, or a
PCR-generated fragment containing a T7 promoter, is added to an aliquot of the
TnT ® Quick Master Mix and incubated in a 50μl reaction volume for 60–90
minutes at 30°C. The expression reaction produces significant quantities of protein
for a variety of applications including GST pull-downs and gel shift assays.
Features:
• Obtain Data Faster: Functional protein is expressed in only one hour, not
days as with cell-based expression systems.
• Multiple Applications with One System: Use expressed protein for the
characterization of protein:protein interaction, protein:nucleic acid interaction,
protein modification and more.
• Consistent, Reliable Results: This mammalian-based system expresses
soluble, functional proteins that are post-translationally modified, unlike
E. coli-based systems.
• Fewer Steps: Expressed proteins can be used directly after expression; no
requirement for additional purification.
• Flexible Systems Available: TnT ® Systems for linear, circular or PCR
templates are available.
Storage Conditions: Store at –70°C. Do not freeze-thaw the lysate more than
two times.
Protocol Part#
TnT ® Quick Coupled Transcription/Translation System Technical
Manual
TM045
For complete and up-to-date product information visit: www.promega.com/catalog
TnT ® Coupled Reticulocyte Lysate Systems
Product Size Cat.# Price ($)
TnT ® SP6 Coupled Reticulocyte Lysate System
TnT
40 reactions L4600 855.00
® SP6 Coupled Reticulocyte Lysate
System, Trial Size 8 reactions L4601 239.00
TnT ® T7 Coupled Reticulocyte Lysate System
TnT
40 reactions L4610 855.00
® T7 Coupled Reticulocyte Lysate System,
Trial Size 8 reactions L4611 239.00
TnT ® T3 Coupled Reticulocyte Lysate System
TnT
40 reactions L4950 855.00
® T7/T3 Coupled Reticulocyte Lysate
System
TnT
40 reactions L5010 914.00
® T7/SP6 Coupled Reticulocyte Lysate
System 40 reactions L5020 914.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The TnT ® Coupled Reticulocyte Lysate Systems offer
researchers an alternative for eukaryotic cell-free protein expression: a singletube,
coupled transcription/translation system. The TnT ® Lysate Systems greatly
simplify the process and reduce the time required to obtain in vitro translation
results. Standard rabbit reticulocyte lysate translations commonly use RNA
synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters and require
three separate reactions with several steps between each reaction. The TnT ®
Systems bypass many of these steps by incorporating transcription directly in
the translation mix.
Features:
• Use in Multiple Applications: The TnT ® Systems are widely used for
protein:protein interaction, protein:nucleic acid interactions, and more.
• Save Time: Using a one-tube reaction, proteins are generated in one hour,
not days, as with in vivo methods.
• Complete System: All the reagents you need are provided (except radioisotopes).
• Reliable: Eliminate solubility issues by using an in vitro mammalian
system.
• Dependability You Can Count On: The TnT ® Systems are rigorously
quality controlled to ensure the highest level of performance.
Storage Conditions: Store the polymerase at –20 to –70°C. Store Luciferase
Assay Wells at room temperature. Store the other components at –70°C. Do
not freeze-thaw the lysate more than two times.
Protocol Part#
TnT ® Coupled Reticulocyte Lysate System Technical Bulletin TB126
223
13
Protein Expression and Analysis
Section
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