2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Eukaryotic Cell-Free Protein
Expression
222
TnT ® T7 Insect Cell Extract Protein Expression
System
Product Size Cat.# Price ($)
TnT ® T7 Insect Cell Extract Protein Expression 10 reactions L1101 294.00
System
40 reactions L1102 1024.00
pF25A ICE T7 Flexi ® Vector 20 µg L1061 478.00
pF25K ICE T7 Flexi ® Vector 20 µg L1081 478.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The TnT ® T7 Insect Cell Extract Protein Expression System is
a convenient, quick, single-tube, coupled transcription/translation system for
the cell-free expression of proteins. Protein synthesis reactions are initiated by
the addition of a DNA template, eliminating the need for the time-consuming
process of in vitro RNA synthesis.
The extract is made from the commonly used Spodoptera frugiperda Sf21 cell
line. All components necessary for the transcription/translation are present in
the TnT ® T7 ICE Master Mix. To initiate protein synthesis, the only component
that must be added is the DNA template. Reactions are incubated at 28–30°C
and are complete within 4 hours.
Proteins are expressed from genes cloned downstream of the T7 promoter.
Companion vectors have been designed to achieve optimal yield with this system
(pF25A and pF25K). They contain untranslated region (UTR) sequences at
the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.
Using the TnT ® T7 Insect Cell Extract Protein Expression System and these
vectors, 75μg/ml of functional protein can be produced.
Features:
• Obtain Data Faster: Protein is expressed in only 4 hours, not days as with
cell-based expression.
• Complete System: No requirement to purchase additional reagents.
• Achieve High Protein Yields: Express up to 75μg/ml of protein for
multiple applications.
Storage Conditions: Store at –70°C.
Protocol Part#
TnT ® T7 Insect Cell Extract System Technical Manual
TM305
pF25A ICE T7 Flexi ® Vector
9PIL106
pF25K ICE T7 Flexi ® Vector
9PIL108
TnT ® SP6 High-Yield Wheat Germ Protein
Expression System
Product Size Cat.# Price ($)
TnT ® SP6 High-Yield Wheat Germ Protein 40 reactions L3260 1012.00
Expression System
Available Separately
10 reactions L3261 321.00
TnT ® SP6 High-Yield Master Mix Minus Amino Acids
For Research Use Only. Not for Use in Diagnostic Procedures.
X808X Pls. Enq.
Description: The TnT ® SP6 High-Yield Wheat Germ Protein Expression
System, based on an optimized wheat germ extract, is a single-tube, coupled
transcription/translation system designed to express proteins in only two hours.
Protein synthesized, in the range of 10–100μg/ml, can be used in multiple
proteomic-based applications, as well as in high-throughput analysis.
All components necessary for transcription/translation are provided in the
extract, with the exception of the plasmid DNA or PCR template. Optional
protein-labeling reagents must also be supplied by the user.
For custom wheat germ extract (depleted amino acids), order Cat.# X808X (see
Products, Available Separately).
Features:
• Save Time: You can generate protein in only two hours, as compared to
days when using cell-based (E. coli) systems.
• Choose Your Format: Use plasmid or PCR-generated templates to
generate protein.
• Achieve High Yields: Generate 10- to 20-fold more protein (10–100μg/ml)
when compared to other cell-free systems.
• Generate Usable Protein: Generate soluble, full-length protein and avoid
problems associated with E. coli systems.
Storage Conditions: Store at –70°C.
Protocol Part#
TnT ® SP6 High-Yield Wheat Germ Protein Expression System
Technical Manual
TM282
For complete and up-to-date product information visit: www.promega.com/catalog