2012 Promega catalogue

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Life Science Catalog 2012 Worldwide Contact List Section Contents Table of Contents Cell Signaling 218 Tth DNA Polymerase Product Size Conc. Cat.# Price ($) Tth DNA Polymerase 100 u 5 u/µl M2101 103.00 For Research Use Only. Not for Use in Diagnostic Procedures. 500 u 5 u/µl M2105 400.00 Description: Tth DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus thermophilus HB-8. The enzyme replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tth DNA Polymerase is recommended for use in PCR, RT-PCR, reverse transcription and primer extension reactions at elevated temperatures. 10X Reverse Transcription Buffer: 100mM Tris-HCl (pH 8.3 at 25°C), 900mM KCl. 10X Chelate Buffer: 100mM Tris-HCl (pH 8.3 at 25°C), 1M KCl, 7.5mM EGTA, 0.5% Tween ® 20, 50% glycerol. Thermophilic DNA Polymerase 10X Reaction Buffer: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C) and 1% Triton ® X-100. Buffer is optimized for use with 0.2mM of each of the dNTPs. Manganese and Magnesium Chloride: 10mM MnCl 2 and 25mM MgCl 2 Solutions provided. Features: • Increased Specificity for RT-PCR: The ability to reverse transcribe at higher temperatures results in increased specificity of primer hybridization and extension. • Minimized Secondary Structures: Higher temperature RT-PCR minimizes problems associated with strong secondary structures in RNA. • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account. Storage Conditions: Store at –20°C. Protocol Part# Tth DNA Polymerase Protocol 9PIM210 Primer Pairs for RT-PCR Product Size Cat.# Price ($) β-Actin Primer Pair 20 reactions G5740 225.00 CNTF Primer Pair 20 reactions G5770 225.00 NT-3 Primer Pair 20 reactions G6801 225.00 p75 Primer Pair 20 reactions G6861 225.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Promega Primer Pairs for RT-PCR provide convenient primers for analyzing the expression of specific mRNAs by RT-PCR. Each Primer Pair includes a 5´ and 3´ oligonucleotide primer provided in individual vials at 100μM ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR (Reverse Transcription System, Cat.# A3500) using rat brain total RNA. They also have been tested in one-step RT-PCR (Access RT-PCR System, Cat.# A1260) with human and mouse brain total RNA. PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate identity. Storage Conditions: Store at –20°C. Universal RiboClone ® cDNA Synthesis System Product Size Cat.# Price ($) Universal RiboClone ® cDNA Synthesis System Available Separately 1 system C4360 938.00 Oligo(dT) 15 Primer 20 µg C1101 114.00 Random Primers 20 µg C1181 87.00 Spin Columns 10 each C1281 76.00 EcoRI Adaptors 150 pmol C1291 76.00 1.2kb Kanamycin Positive Control RNA 5 µg C1381 141.00 Sephacryl ® S-400 10 ml V3181 76.00 C1181, C1381, V3181 For Laboratory Use. C1101, C4360, C1281, C1291 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Universal RiboClone ® cDNA Synthesis System contains the reagents required for the synthesis of double-stranded cDNA from mRNA and subsequent ligation into a suitable vector. The system is based on the method described by Okayama and Berg with modifications by Gubler and Hoffman. First-strand synthesis is driven by AMV (Avian Myeloblastosis Virus) Reverse Transcriptase and either Random Primers or an Oligo(dT) 15 Primer, followed directly by second-strand replacement synthesis using RNase H and DNA Polymerase I. After treatment with T4 DNA Polymerase to flush the ends, the double-stranded cDNA molecules are prepared for cloning by size fractionation and the addition of EcoRI Adaptors. The resulting cDNA preparation can then be cloned into a suitable vector. Features: • Convenient: Contains all the necessary reagents to synthesize doublestranded cDNA from RNA. • Flexible: Both Oligo(dT) 15 Primer and Random Primers are included, providing the researcher a choice of priming methods. Storage Conditions: Store control RNA at –70°C. Store Sephacryl ® S-400 at 4°C and Spin Columns at room temperature. Store other components at –20°C. Protocol Part# Universal RiboClone ® cDNA Synthesis System Technical Manual TM038 Oligonucleotides and Primers: cDNA Synthesis and Cloning Product Size Cat.# Price ($) Oligo(dT) 15 Primer 20 µg C1101 114.00 Random Primers 20 µg C1181 87.00 EcoRI Adaptors 150 pmol C1291 76.00 C1181 For Laboratory Use. C1101, C1291 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Oligo(dT) 15 Primer is suitable for the use of a primer for firststrand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly(A) tail of mRNA. Random Primers can be used for first-strand cDNA synthesis and cloning; they are also available as components of the Universal Riboclone ® cDNA Synthesis System (Cat.# C4360) and the Reverse Transcription System (Cat.# A3500). The primers are random hexadeoxynucleotides. The EcoRI Adaptors consist of two complementary oligonucleotides: a 16mer and a 12mer phosphorylated at the 5´-end. The oligonucleotides are provided annealed in equimolar concentrations in water. The EcoRI Adaptors are designed to attach EcoRI “sticky” ends to blunt-ended DNA. Storage Conditions: Store at –20°C. For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling PCR Cloning pGEM ® -T Vector Systems Product Size Cat.# Price ($) pGEM ® -T Vector System I 20 reactions A3600 268.00 pGEM ® -T Vector System II 20 reactions A3610 448.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM ® -T Vector Systems are convenient systems for the cloning of PCR products. The pGEM ® -T Vector is prepared by cutting the pGEM ® -5Zf(+) Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for ligation of PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template- independent fashion, to the 3´-ends of amplified fragments. The multiple cloning site is flanked by recognition sites for the restriction enzyme BstZI, allowing release of the insert by a single-enzyme digestion. Alternatively, a double digestion may be used to release the insert from the vector. The pGEM ® -T Vector System II contains JM109 Competent Cells in addition to all of the pGEM ® -T Vector System I components. Features: • Rapid Ligation: The 2X Rapid Ligation Buffer provided allows reactions to be completed in 1 hour at room temperature. • Blue/White Screening: T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by color screening on indicator plates. • f1 Origin of Replication: Allows the preparation of single-stranded DNA. Storage Conditions: Store competent cells at –70°C; store all other components at –20°C. Protocol Part# TM042 pGEM ® -T and pGEM ® -T Easy Vector Systems Technical Manual XmnI 1994 ScaI 1875 Amp r 1. Add PCR product to T-Vector. 4. Transform Competent Cells. pGEM ® -T Vector (3000bp) ori NaeI 2692 f1 ori T7 1 start ApaI 14 AatII 20 SphI 26 BstZI 31 lacZ T T NcoI SacII 37 46 SpeI 55 NotI 62 BstZI 62 PstI 73 SalI 75 NdeI 82 SacI 94 BstXI 103 NsiI 112 SP6 126 For complete and up-to-date product information visit: www.promega.com/catalog ➞ ➞ 2. Add 2X Rapid Ligation Buffer and T4 DNA Ligase. 3. Incubate 1 hour. The rapid ligation reaction reduces ligation time to just 60 minutes. 1985MD 0356VA04_3A pGEM ® -T Easy Vector Systems Product Size Cat.# Price ($) pGEM ® -T Easy Vector System I 20 reactions A1360 282.00 pGEM ® -T Easy Vector System II 20 reactions A1380 463.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM ® -T Easy Vector Systems are convenient systems for the cloning of PCR products. They offer all of the advantages of the pGEM ® -T Vector Systems with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus several options for removal of the desired insert DNA with a single restriction digestion are provided. The pGEM ® -T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM ® -T Easy Vector System I components. Features: • Flexible: The multiple cloning site is flanked by restriction enzyme sites for BstZI, NotI and EcoRI, allowing three options for removal of the insert with a single digest. • Rapid Ligation: The 2X Rapid Ligation Buffer provided allows reactions to be completed in 1 hour at room temperature. • Blue/White Screening: T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by color screening on indicator plates. • f1 Origin of Replication: Allows the preparation of single-stranded DNA. Storage Conditions: Store competent cells at –70°C; store all other components at –20°C. Protocol Part# TM042 pGEM ® -T and pGEM ® -T Easy Vector Systems Technical Manual XmnI 2009 ScaI 1890 Total Colonies Amp r 2,000 1,800 1,600 1,400 1,200 1,000 800 pGEM ® -T Easy Vector (3015bp) ori f1 ori NaeI 2707 lacZ T T Recombinant colonies Nonrecombinant colonies T7 ApaI AatII SphI BstZI NcoI BstZI NotI SacII EcoRI ➞ SpeI EcoRI NotI BstZI PstI SalI NdeI SacI BstXI NsiI ➞ SP6 90% Recombinants 1 start 14 20 26 31 37 43 43 49 52 64 70 77 77 88 90 97 109 118 127 141 600 67% 400 Recombinants 200 0 4382MA11_3A Unpurified Purified Purification of PCR products enhances cloning success. A 500bp PCR product was purified with the Wizard ® SV Gel and PCR Clean-Up System and cloned into the pGEM ® -T Easy Vector. Both the percent recombinants and total number of colonies increase with a pure PCR product. White bars represent recombinant colonies. Blue bars represent nonrecombinant colonies. 1473VA05_6A 219 12 PCR Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery For
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity F
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
Page 171 and 172: Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174: Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176: Cell Signaling Anti-Human GDNF pAb
Page 177 and 178: Cell Signaling Anti-PARP p85 Fragme
Page 179 and 180: Cell Signaling Anti-VAChT pAb Produ
Page 181 and 182: Cell Signaling VivoGlo Caspase 3/7
Page 183 and 184: Cell Signaling For complete and up-
Page 185 and 186: Cell Signaling Microbial Detection
Page 187 and 188: 10 Instruments For complete and up-
Page 189 and 190: GloMax ® 20/20 Luminometer Product
Page 191 and 192: Fluorescence Module: Application-op
Page 193 and 194: GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196: Maxwell ® 16 Instruments Maxwell
Page 197 and 198: Maxwell ® 16 Flexi Method Firmware
Page 201 and 202: Cell Signaling Maxwell ® 16 System
Page 203 and 204: Cell Signaling Maxwell ® 16 Flexi
Page 205 and 206: Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR For complete
Page 209 and 210: Cell Signaling TaqBead Hot Start Po
Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
Page 219 and 220: Cell Signaling Reverse Transcriptio
Page 221: Cell Signaling AMV Reverse Transcri
Page 227 and 228: Cell Signaling TnT ® Quick Coupled
Page 229 and 230: Cell Signaling TnT ® T7 Quick for
Page 231 and 232: Cell Signaling Canine Pancreatic Mi
Page 233 and 234: Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236: Cell Signaling Immobilized Trypsin
Page 237 and 238: Cell Signaling Asp-N, Sequencing Gr
Page 239 and 240: Cell Signaling HaloTag ® Ligand Bu
Page 241 and 242: Cell Signaling Inducible T7-Driven
Page 243 and 244: Cell Signaling ProtoBlot ® II AP S
Page 247 and 248: Cell Signaling HaloTag ® Protein P
Page 249 and 250: Cell Signaling HaloTag ® Mammalian
Page 251 and 252: Cell Signaling HaloLink Protein Arr
Page 253 and 254: Cell Signaling HaloCHIP System Prod
Page 255 and 256: Cell Signaling MagneHis Protein Pur
Page 257 and 258: Cell Signaling Streptavidin Product
Page 259 and 260: Cell Signaling Protein Purification
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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2012 Promega catalogue

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