2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
218
Tth DNA Polymerase
Product Size Conc. Cat.# Price ($)
Tth DNA Polymerase 100 u 5 u/µl M2101 103.00
For Research Use Only. Not for Use in Diagnostic Procedures.
500 u 5 u/µl M2105 400.00
Description: Tth DNA Polymerase is a thermostable enzyme of approximately
94kDa isolated from Thermus thermophilus HB-8. The enzyme replicates DNA
at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth DNA Polymerase
catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´
direction in the presence of magnesium and the polymerization of nucleotides
into DNA using an RNA template in the 5´→3´ direction in the presence of
manganese. The enzyme also possesses a 5´→3´ exonuclease activity.
Tth DNA Polymerase is recommended for use in PCR, RT-PCR, reverse
transcription and primer extension reactions at elevated temperatures.
10X Reverse Transcription Buffer: 100mM Tris-HCl (pH 8.3 at 25°C),
900mM KCl.
10X Chelate Buffer: 100mM Tris-HCl (pH 8.3 at 25°C), 1M KCl,
7.5mM EGTA, 0.5% Tween ® 20, 50% glycerol.
Thermophilic DNA Polymerase 10X Reaction Buffer: 500mM KCl,
100mM Tris-HCl (pH 9.0 at 25°C) and 1% Triton ® X-100. Buffer is
optimized for use with 0.2mM of each of the dNTPs.
Manganese and Magnesium Chloride: 10mM MnCl 2 and 25mM MgCl 2
Solutions provided.
Features:
• Increased Specificity for RT-PCR: The ability to reverse transcribe at
higher temperatures results in increased specificity of primer hybridization
and extension.
• Minimized Secondary Structures: Higher temperature RT-PCR
minimizes problems associated with strong secondary structures in RNA.
• Performance Guarantee: Promega PCR systems, enzymes and reagents
are proven in PCR to ensure reliable, high-performance results. If you are
not completely satisfied with any Promega PCR product, we will send a
replacement or refund your account.
Storage Conditions: Store at –20°C.
Protocol Part#
Tth DNA Polymerase Protocol 9PIM210
Primer Pairs for RT-PCR
Product Size Cat.# Price ($)
β-Actin Primer Pair 20 reactions G5740 225.00
CNTF Primer Pair 20 reactions G5770 225.00
NT-3 Primer Pair 20 reactions G6801 225.00
p75 Primer Pair 20 reactions G6861 225.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Promega Primer Pairs for RT-PCR provide convenient primers
for analyzing the expression of specific mRNAs by RT-PCR. Each Primer Pair
includes a 5´ and 3´ oligonucleotide primer provided in individual vials at
100μM ready for addition to the amplification reaction. The Primer Pairs have
been confirmed in two-step conventional RT-PCR (Reverse Transcription
System, Cat.# A3500) using rat brain total RNA. They also have been tested
in one-step RT-PCR (Access RT-PCR System, Cat.# A1260) with human and
mouse brain total RNA.
PCR products from the use of the Primer Pairs have been sequence-confirmed
for appropriate identity.
Storage Conditions: Store at –20°C.
Universal RiboClone ® cDNA Synthesis System
Product Size Cat.# Price ($)
Universal RiboClone ® cDNA Synthesis System
Available Separately
1 system C4360 938.00
Oligo(dT) 15 Primer 20 µg C1101 114.00
Random Primers 20 µg C1181 87.00
Spin Columns 10 each C1281 76.00
EcoRI Adaptors 150 pmol C1291 76.00
1.2kb Kanamycin Positive Control RNA 5 µg C1381 141.00
Sephacryl ® S-400 10 ml V3181 76.00
C1181, C1381, V3181 For Laboratory Use. C1101, C4360, C1281, C1291 For Research Use
Only. Not for Use in Diagnostic Procedures.
Description: The Universal RiboClone ® cDNA Synthesis System contains the
reagents required for the synthesis of double-stranded cDNA from mRNA and
subsequent ligation into a suitable vector. The system is based on the method
described by Okayama and Berg with modifications by Gubler and Hoffman.
First-strand synthesis is driven by AMV (Avian Myeloblastosis Virus) Reverse
Transcriptase and either Random Primers or an Oligo(dT) 15 Primer, followed
directly by second-strand replacement synthesis using RNase H and DNA
Polymerase I. After treatment with T4 DNA Polymerase to flush the ends, the
double-stranded cDNA molecules are prepared for cloning by size fractionation
and the addition of EcoRI Adaptors. The resulting cDNA preparation can then be
cloned into a suitable vector.
Features:
• Convenient: Contains all the necessary reagents to synthesize doublestranded
cDNA from RNA.
• Flexible: Both Oligo(dT) 15 Primer and Random Primers are included,
providing the researcher a choice of priming methods.
Storage Conditions: Store control RNA at –70°C. Store Sephacryl ® S-400
at 4°C and Spin Columns at room temperature. Store other components at
–20°C.
Protocol Part#
Universal RiboClone ® cDNA Synthesis System Technical Manual TM038
Oligonucleotides and Primers: cDNA Synthesis
and Cloning
Product Size Cat.# Price ($)
Oligo(dT) 15 Primer 20 µg C1101 114.00
Random Primers 20 µg C1181 87.00
EcoRI Adaptors 150 pmol C1291 76.00
C1181 For Laboratory Use. C1101, C1291 For Research Use Only. Not for Use in Diagnostic
Procedures.
Description: Oligo(dT) 15 Primer is suitable for the use of a primer for firststrand
cDNA synthesis with a reverse transcriptase. The primer hybridizes to
the poly(A) tail of mRNA.
Random Primers can be used for first-strand cDNA synthesis and cloning;
they are also available as components of the Universal Riboclone ® cDNA
Synthesis System (Cat.# C4360) and the Reverse Transcription System
(Cat.# A3500). The primers are random hexadeoxynucleotides.
The EcoRI Adaptors consist of two complementary oligonucleotides: a 16mer
and a 12mer phosphorylated at the 5´-end. The oligonucleotides are provided
annealed in equimolar concentrations in water. The EcoRI Adaptors are
designed to attach EcoRI “sticky” ends to blunt-ended DNA.
Storage Conditions: Store at –20°C.
For complete and up-to-date product information visit: www.promega.com/catalog