2012 Promega catalogue

Cell Signaling
AMV Reverse Transcriptase
Product Size Conc. Cat.# Price ($)
AMV Reverse Transcriptase 300 u 10 u/µl M5101 111.00
1,000 u 10 u/µl M5108 293.00
AMV Reverse Transcriptase (HC) 600 u 20–25 u/µl M9004 223.00
For Laboratory Use.
Description: Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT)
catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA
hybrids. It requires a primer (DNA primers are more efficient than RNA primers)
as well as Mg 2+ or Mn 2+ . The enzyme possesses an intrinsic RNase H activity.
Both nonionic detergents and sulfhydryl compounds stabilize the enzyme
activity in vitro.
Features:
• Available at High Concentration: Cat.# M9004 contains 600 units of
AMV RT at 20–25u/μl.
• Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C),
250mM KCl, 50mM MgCl 2, 2.5mM spermidine, 50mM DTT.
• Temperature Stability: AMV RT is the preferred reverse transcriptase
for templates with high secondary structure due to its stability at higher
reaction temperatures (37–58°C).
Storage Conditions: Store at –20°C.
Protocol Part#
AMV Reverse Transcriptase Protocol 9PIM510
M-MLV Reverse Transcriptase
Product Size Conc. Cat.# Price ($)
M-MLV Reverse Transcriptase 10,000 u 200 u/µl M1701 165.00
M-MLV Reverse Transcriptase Buffer
50,000 u 200 u/µl M1705 586.00
Pack
For Laboratory Use.
2 × 1 ml M5313 42.00
Description: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT)
is an RNA-dependent DNA polymerase that can be used in cDNA synthesis
with long messenger RNA templates (>5kb). The enzyme is a product of the
pol gene of M-MLV and consists of a single subunit with a molecular weight of
71kDa. The RNase H activity of M-MLV RT is weaker than the commonly used
Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features:
• Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C),
375mM KCl, 15mM MgCl 2, 50mM DTT.
• May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C
for 10 minutes.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol Part#
M-MLV Reverse Transcriptase Protocol 9PIM170
For complete and up-to-date product information visit: www.promega.com/catalog
M-MLV Reverse Transcriptase,
RNase H Minus
Product
M-MLV Reverse Transcriptase,
Size Conc. Cat.# Price ($)
RNase H Minus 10,000 u 100–200 u/µl M5301 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in
all countries. Please contact your local representative for more information.
Description: Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H
Minus (M-MLV RT [H–]), is an RNA-dependent DNA polymerase that can be
used in cDNA synthesis with long messenger RNA templates (>5kb). This is
a form of M-MLV Reverse Transcriptase that has been genetically altered
to remove the associated RNase H activity. Although many researchers are
successful in using M-MLV RT (H+) for analytical and some preparative
cDNA applications, reverse transcriptases lacking RNase H activity provide
another option for the preparation of long cDNAs and libraries containing a
high percentage of full-length cDNA.
Features:
• RNase H Minus: Provides optimal conditions for the preparation of fulllength
cDNA from long RNA templates.
• Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C),
375mM KCl, 15mM MgCl 2, 50mM DTT.
• May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C
for 10 minutes.
Storage Conditions: Store at –20°C.
Protocol Part#
M-MLV Reverse Transcriptase, RNase H Minus Protocol 9PIM530
M-MLV Reverse Transcriptase,
RNase H Minus, Point Mutant
Product Size Cat.# Price ($)
M-MLV Reverse Transcriptase,
2,500 u M3681 Pls. Enq.
RNase H Minus, Point Mutant
10,000 u M3682 Pls. Enq.
50,000 u M3683 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in
all countries. Please contact your local representative for more information.
Description: Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H
Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase
that can be used in cDNA synthesis with long RNA templates (>5kb). The lack
of RNase H activity is beneficial for this application, as RNase H can start to
degrade templates when incubation times are long, as they may be when
making long cDNAs. Although many researchers are successful in using
M-MLV RT (H+) for analytical and some preparative cDNA applications,
reverse transcriptases lacking RNase H activity provide another option for
the preparation of long cDNAs and for libraries containing a high percentage
of full-length cDNA.
Features:
• RNase H Minus: Provides optimal conditions for the preparation of fulllength
cDNA from long RNA templates.
• Temperature Stability: Thermostability of this point mutant prevents
problems associated with secondary structure.
• Increased Polymerase Activity: M-MLV RT (H–), Point Mutant, gives
higher yields of cDNA compared with the deletion mutant (Cat.# M5301).
• Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C),
375mM KCl, 15mM MgCl 2, 50mM DTT.
• Broad Working Range: More tolerance to variations in enzyme and
substrate concentration means improved consistency in performance.
Storage Conditions: Store at –20°C.
Protocol Part#
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant Protocol 9PIM368
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