2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
216
Access RT-PCR System
Product Size Cat.# Price ($)
Access RT-PCR Introductory System 20 reactions A1260 196.00
Access RT-PCR System 100 reactions A1250 768.00
For Research Use Only. Not for Use in Diagnostic Procedures.
500 reactions A1280 3000.00
Description: The Access RT-PCR System is designed for reverse transcription
(RT) and polymerase chain reaction (PCR) amplification of a specific target RNA
from either total RNA or mRNA. This one-tube, two-enzyme system provides
sensitive, quick, and reproducible analysis of even rare RNAs. The system
uses AMV Reverse Transcriptase (AMV RT) from Avian Myeloblastosis Virus
for first-strand DNA synthesis and the thermostable Tfl DNA Polymerase from
Thermus flavus for second-strand cDNA synthesis and DNA amplification. The
Access RT-PCR System includes an optimized single-buffer system that permits
extremely sensitive detection of RNA transcripts, without a requirement for
buffer additions between the reverse transcription and PCR amplification steps.
This simplifies the procedure and reduces the potential for contaminating the
samples. In addition, the improved performance of AMV Reverse Transcriptase
at elevated temperatures in AMV/Tfl 5X Reaction Buffer minimizes problems
encountered with secondary structures in RNA.
Features:
• Achieve Maximum Control: Separate tubes of each component allow
you to control every step of the reaction. You can optimize Mg 2+ , perform
no-reverse transcriptase control reactions, etc.
• Use Less Template: Detect message from as little as 1pg of total RNA or
mRNA.
• No Buffer Additions Required: The AMV/Tfl 5X Reaction Buffer provides
results in optimal enzyme activity without buffer additions between the
reverse transcription and DNA amplification steps.
• Rely on a Performance-Tested System: Promega PCR Systems,
enzymes and reagents are proven in PCR to ensure reliable, high-
performance results. If you are not completely satisfied with any Promega
PCR product, we will send a replacement or refund your account.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store all system components at –20°C. For long-term
storage, the Positive Control RNA with Carrier must be stored at –70°C.
Protocol Part#
Access RT-PCR System Technical Bulletin TB220
Access
RT-PCR
System
Tfl DNA
Polymerase
dNTPs
5X
AMV/Tfl
Buffer
MgSO 4
AMV
Reverse
Transcriptase
Template
RNA
AccessQuick
RT-PCR
System
AccessQuick
Master Mix
Separate AMV RT
allows all-important
no-RT control reaction
AMV
Reverse
Transcriptase
Gene-specific
primers
One-Step
RT-PCR
Features of Access and AccessQuick RT-PCR Systems.
Access AccessQuick
RT-PCR System RT-PCR System
Maximum Control Maximum Convenience
Components Individual tubes of Tfl DNA Polymerase,
Tfl DNA Polymerase, dNTPs and reaction
AMV RT, dNTPs buffer combined in
and reaction buffer master mix. AMV RT
in separate tube
Mg 2+ Concentration Adjustable 1.5mM
Controls Included Yes No
For complete and up-to-date product information visit: www.promega.com/catalog
3777MA07_2A
9478LA