2012 Promega catalogue

Cell Signaling
Reverse Transcription System
Product Size Cat.# Price ($)
Reverse Transcription System 100 reactions A3500 685.00
Available Separately Size Conc. Cat.# Price ($)
Magnesium Chloride Solution 1.5 ml 25 mM A3511 46.00
Reverse Transcription 10X Buffer 1.4 ml A3561 58.00
A3511, A3561 For Laboratory Use. A3500 For Research Use Only. Not for Use in Diagnostic
Procedures.
Description: The Reverse Transcription System provides reagents to efficiently
reverse transcribe RNA into cDNA in fifteen minutes. The cDNA prepared from
each reaction using this system may be used directly in multiple PCR
amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase
synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT) 15
and Random Primers are included, allowing cDNA synthesis from virtually any
RNA source. The system contains sufficient reagents for 100 cDNA synthesis
reactions, processing 1μg of RNA per reaction. Each cDNA synthesis reaction
may be divided and used in up to 20 separate PCR amplifications. A polyadenylated
1.2kb RNA transcript is provided as a control template for the
cDNA synthesis reaction.
Features:
• Fast: Efficiently reverse transcribe poly(A)+ mRNA or total RNA in fifteen
minutes.
• Convenient: PCR-compatible components are provided in optimized
volumes for 100 reactions.
• Positive Controls: A polyadenylated RNA transcript is provided to help
troubleshoot RT-PCR parameters.
Storage Conditions: Store at –20°C. Store Positive Control RNA at –70°C.
Protocol Part#
Reverse Transcription System Technical Bulletin TB099
For complete and up-to-date product information visit: www.promega.com/catalog
AccessQuick RT-PCR System
Product Size Cat.# Price ($)
AccessQuick RT-PCR System 20 reactions A1701 208.00
100 reactions A1702 805.00
500 reactions A1703 2984.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The AccessQuick RT-PCR System is an easy and convenient
master mix system for setting up one-tube RT-PCR. It is designed to increase
the convenience of performing RT-PCR by combining these components in
a single tube: Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction
buffer. The AMV RT enzyme is provided in a separate tube to allow important,
no-RT control reactions. The AccessQuick Master Mix is simply added to
RNA templates in reaction vials, followed by the AMV RT, primers and water.
The AccessQuick RT-PCR Master Mix is intended for routine RT-PCR
applications that have been previously optimized and do not require extreme
conditions.
Features:
• Achieve Maximum Convenience: Save yourself four pipetting steps.
Simply combine the AccessQuick Master Mix, AMV RT, your genespecific
primers, your RNA template and water. Separate AMV RT allows
important, no-RT control reactions.
• Use Less Template: Amplify from zeptomole (10 –21 mol) levels of RNA.
• No Buffer Additions Required: Set up reactions in a single tube, drop
into a thermal cycler, come back later for results—no additions between
the reverse transcription and DNA amplification steps.
• Stable: System components are stable over many freeze-thaw cycles.
Storage Conditions: Store all system components at –20°C.
Protocol Part#
AccessQuick RT-PCR System Protocol 9PIA170
bp
323 –
zeptomoles RNA per reaction
0 5 10
M M
0
0
2,500
25
2.5 0.25
2,500
25
2.50.25
0
2,500
25
2.5
0.25
Stability of AccessQuick Master Mix through multiple freeze-thaw
events. Rapid freeze-thaw events were performed 0, 5 and 10 times by
removing a sample of the AccessQuick Master Mix from –70°C storage
and placing it in a 50°C heat block. After 5 cycles, and again after 10 cycles,
we added AMV RT, primers and RNA. All samples were then used in RT-PCR
reactions to amplify a 323bp fragment using the indicated amounts of the
1.2kb Kanamycin Positive Control RNA (Cat.# C1381) template. Lane M =
100bp DNA Ladder (Cat.# G2101).
3511TB
215
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PCR
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