2012 Promega catalogue

Cell Signaling
StemElite Differentiation-Associated
Transcripts
Product Size Cat.# Price ($)
StemElite FOXA2/GAPDH Primer Pair (20X) 100 µl B1341 134.00
StemElite SOX17/GAPDH Primer Pair (20X) 100 µl B1351 134.00
StemElite GATA6/GAPDH Primer Pair (20X) 100 µl B1361 134.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Pluripotential stem cells can give rise to differentiated cells and
tissues for all three embryonic germ layers. FOXA2, SOX17 and GATA6 are
nonspecific differentiation markers.
Features:
• Achieve sensitive real-time detection and relative quantification of RNA
expression levels for monitoring stem cell differentiation in a multiplexed
amplification.
• Quantitatively amplify a two-color duplex.
• Amplify in a single tube the transcript of interest and reference transcript
(GAPDH).
• Reduce the number of reactions required to measure multiple transcripts.
• Improve experimental data quality by measuring all of the transcripts in the
same well.
Storage Conditions: Store at –20°C.
StemElite Mouse Pluripotent Transcripts
Product Size Cat.# Price ($)
StemElite Mus-Nanog/Actb Primer Pair (20X) 100 µl B1371 134.00
StemElite Mus-Sox2/Actb Primer Pair (20X) 100 µl B1381 134.00
StemElite Mus-Pou5f1/Actb Primer Pair (20X) 100 µl B1391 134.00
StemElite Mus-Lin28/Actb Primer Pair (20X) 100 µl B1401 134.00
StemElite Mus-Klf4/Actb Primer Pair (20X) 100 µl B1411 134.00
StemElite Mus-Myc/Actb Primer Pair (20X)
For Research Use Only. Not for Use in Diagnostic Procedures.
100 µl B1421 134.00
Description: Mus-Nanog, Mus-Sox2, Mus-Pou5f1, Mus-Lin28, Mus-Klf4 and
Mus-Myc are functionally associated with maintenance of the undifferentiated
mouse embryonic stem cell.
Features:
• Achieve sensitive real-time detection and relative quantification of RNA
expression levels for monitoring stem cell differentiation in a multiplexed
amplification.
• Quantitatively amplify a two-color duplex.
• Amplify in a single tube the transcript of interest and reference transcript
(Actb).
• Reduce the number of reactions required to measure multiple transcripts.
• Improve experimental data quality by measuring all of the transcripts in the
same well.
Storage Conditions: Store at –20°C.
RT-PCR
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GoScript Reverse Transcription System
Product Size Cat.# Price ($)
GoScript Reverse Transcription System 50 reactions A5000 450.00
Available Separately
100 reactions A5001 800.00
GoScript Reverse Transcriptase 100 reactions A5003 388.00
For Laboratory Use.
500 reactions A5004 1450.00
Description: The GoScript Reverse Transcription System includes a reverse
transcriptase and a specialized set of reagents designed for efficient synthesis
of first-strand cDNA optimized for quantitative PCR amplification. GoScript
Reverse Transcriptase utilizes M-MLV and state-of-the-art buffer technology
designed for qPCR to deliver robust, reliable cDNA synthesis of a full range of
rare and abundant transcripts, even in the presence of inhibitors. GoScript
Reverse Transcriptase is qualified for use in qPCR, including GoTaq ® qPCR and
Plexor ® qPCR systems for performing RT-qPCR.
Features:
• Ultra-Active: Save money on every reaction.
• Sensitive: Detect rare transcripts.
• Processive: Transcribe long messages.
• Resilient: Synthesize cDNA in the presence of strong inhibitors.
• RT Replaces Superscript ® I, II, III and other RTs.
Storage Conditions: Store at –20°C.
Protocol Part#
GoScript Reverse Transcription System Technical Manual TM316
213
12
PCR
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