2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
212
StemElite Gene Expression System
Product Size Cat.# Price ($)
StemElite Gene
Expression System 100 qPCR reactions B1001 258.00
StemElite Gene
Expression System Plus
For Research Use Only. Not for Use in Diagnostic Procedures.
100 qPCR reactions
+ 50 RT reactions B1002 423.00
Description: The StemElite Gene Expression System is a novel real-time
quantitative PCR (qPCR) system for the detection and relative quantification of
RNA expression levels associated with the differentiation state or ‘potency’ of
cells. The StemElite Gene Expression System is optimized to quantitatively
amplify a two-color duplex, enabling the user to amplify a transcript of interest
as well as a reference gene in a single reaction.
Features:
• Achieve sensitive real-time detection and relative quantification of RNA
expression levels for monitoring stem cell differentiation in a multiplexed
amplification.
• Quantitatively amplify a two-color duplex.
• Amplify in a single tube the transcript of interest and reference transcript
(GAPDH or Actb).
• Reduce the number of reactions required to measure multiple transcripts.
• Improve experimental data quality by measuring all of the transcripts in the
same well.
Storage Conditions: Store at –20°C.
Protocol Part#
StemElite Gene Expression System Technical Manual TM323
StemElite Human Pluripotent Transcripts
Product Size Cat.# Price ($)
StemElite NANOG/GAPDH Primer Pair (20X) 100 µl B1011 134.00
StemElite SOX2/GAPDH Primer Pair (20X) 100 µl B1021 134.00
StemElite POU5F1/GAPDH Primer Pair (20X) 100 µl B1031 134.00
StemElite LIN28/GAPDH Primer Pair (20X) 100 µl B1041 134.00
StemElite KLF4/GAPDH Primer Pair (20X) 100 µl B1051 134.00
StemElite MYC/GAPDH Primer Pair (20X)
For Research Use Only. Not for Use in Diagnostic Procedures.
100 µl B1061 134.00
Description: NANOG, SOX2, POU5F1, LIN28, KLF4 and MYC are functionally
associated with maintenance of the undifferentiated human embryonic stem
cell.
Features:
• Achieve sensitive real-time detection and relative quantification of RNA
expression levels for monitoring stem cell differentiation in a multiplexed
amplification.
• Quantitatively amplify a two-color duplex.
• Amplify in a single tube the transcript of interest and reference transcript
(GAPDH).
• Reduce the number of reactions required to measure multiple transcripts.
• Improve experimental data quality by measuring all of the transcripts in the
same well.
Storage Conditions: Store at –20°C.
StemElite Human Heart-Associated
Transcripts
Product Size Cat.# Price ($)
StemElite NPPA/GAPDH Primer Pair (20X) 100 µl B1071 134.00
StemElite MYL7/GAPDH Primer Pair (20X) 100 µl B1081 134.00
StemElite MYL2/GAPDH Primer Pair (20X) 100 µl B1091 134.00
StemElite MYH6/GAPDH Primer Pair (20X) 100 µl B1101 134.00
StemElite MYH7/GAPDH Primer Pair (20X) 100 µl B1111 134.00
StemElite NKX2-5/GAPDH Primer Pair (20X) 100 µl B1121 134.00
StemElite TNNT2/GAPDH Primer Pair (20X) 100 µl B1131 134.00
StemElite TNNI3/GAPDH Primer Pair (20X) 100 µl B1141 134.00
StemElite MEF2C/GAPDH Primer Pair (20X) 100 µl B1151 134.00
StemElite PLN/GAPDH Primer Pair (20X) 100 µl B1161 134.00
StemElite GATA4/GAPDH Primer Pair (20X)
For Research Use Only. Not for Use in Diagnostic Procedures.
100 µl B1171 134.00
Description: Pluripotential stem cells can give rise to differentiated cells and
tissues for all three embryonic germ layers. NPPA, MYL7, MYL2, MYH6, MYH7,
NKX2-5, TNNT2, TNNI3, MEF2C, PLN and GATA4 are mesodermal markers
associated with the differentiation of cardiac muscle.
Features:
• Achieve sensitive real-time detection and relative quantification of RNA
expression levels for monitoring stem cell differentiation in a multiplexed
amplification.
• Quantitatively amplify a two-color duplex.
• Amplify in a single tube the transcript of interest and reference transcript
(GAPDH).
• Reduce the number of reactions required to measure multiple transcripts.
• Improve experimental data quality by measuring all of the transcripts in the
same well.
Storage Conditions: Store at –20°C.
StemElite Human Pancreatic-Associated
Transcripts
Product Size Cat.# Price ($)
StemElite HNF4A/GAPDH Primer Pair (20X) 100 µl B1301 134.00
StemElite HNF1B/GAPDH Primer Pair (20X) 100 µl B1311 134.00
StemElite PDX1/GAPDH Primer Pair (20X) 100 µl B1321 134.00
StemElite INS/GAPDH Primer Pair (20X)
For Research Use Only. Not for Use in Diagnostic Procedures.
100 µl B1331 134.00
Description: Pluripotential stem cells can give rise to differentiated cells and
tissues for all three embryonic germ layers. HNF4A, HNF1B, PDX1 and INS are
mesodermal markers associated with the differentiation of pancreatic cells.
Features:
• Achieve sensitive real-time detection and relative quantification of RNA
expression levels for monitoring stem cell differentiation in a multiplexed
amplification.
• Quantitatively amplify a two-color duplex.
• Amplify in a single tube the transcript of interest and reference transcript
(GAPDH).
• Reduce the number of reactions required to measure multiple transcripts.
• Improve experimental data quality by measuring all of the transcripts in the
same well.
Storage Conditions: Store at –20°C.
For complete and up-to-date product information visit: www.promega.com/catalog