2012 Promega catalogue

Cell Signaling
Plexor ® qPCR and qRT-PCR Systems
Product Size Cat.# Price ($)
Plexor ® qPCR System 200 reactions A4011 288.00
Plexor ® One-Step qRT-PCR System 200 reactions A4021 488.00
Plexor ® Two-Step qRT-PCR System 200 reactions A4051 576.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Plexor ® qPCR and qRT-PCR Systems are multiplex-capable
real-time amplification systems using novel base pair chemistry. Each target is
measured directly during the amplification process and not through a secondary
reaction. Plexor ® reactions require only two primers for each target. Multiplex
assay design is further simplified by the use of the web-based Plexor ® Primer
Design program specifically engineered for multiplex assay design.
The Plexor ® Systems work by measuring a reduction in fluorescent signal
during amplification. Amplification uses only two primers, one of which
contains both a fluorescent tag and a modified base. As amplification proceeds,
fluorescence is reduced by site-specific incorporation of a fluorescent quencher
inserted opposite the complementary modified base. The quencher is in close
proximity to a fluorescent dye located on the end of the primer, resulting in a
reduction in the fluorescent signal. After PCR, a melt analysis can be performed
to provide an internal control for the final assay design or to expedite troubleshooting
during development. The system also includes a proprietary reagent
to minimize primer-dimer formation.
Features:
• Multiplex Performance: Only two primers are required for each target,
making the design of multiplex assays much easier.
• Improve Productivity: Less labor, less time and less cost per assay.
Measure controls and targets at the same time in the same well.
• Enjoy Convenience: The master mix format provides everything you
need in one tube. Combine with your template and primers.
• Obtain Strong Data: Measure a reduction in fluorescent signal during
amplification. Quenching is directly proportional to amplicon accumulation.
• Use Your Existing Real-Time Instruments: Works on most real-time
instruments capable of measuring more than one fluor. Import and analyze
data from your preferred instrument platform.
• Three Simple Steps to Use Plexor ® Systems:
Step 1. Design Your Assay: Choose your primer sets, then order from
your preferred oligo provider.
Step 2. Run the Assay: Instruction manuals are available for a wide
variety of real-time instruments including Applied Biosystems and Roche.
All Plexor ® assays are designed for the same cycling conditions.
Step 3. Analyze Your Data: Export the raw data from your real-time
instrument, then import into our free Plexor ® Analysis Software. The
software converts the quenching data into cycle threshold (C t) values and
generates standard curves.
Storage Conditions: Store at –20°C.
Protocol Part#
Plexor ® qPCR System Technical Manual
TM262
Plexor ® One-Step qRT-PCR System Technical Manual
TM263
Plexor ® Two-Step qRT-PCR System Technical Manual
TM264
MOPS/EDTA Buffer
Product Size Cat.# Price ($)
MOPS/EDTA Buffer 3 × 10 ml Y5101 66.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: MOPS/EDTA Buffer is provided at pH 7.4 for the resuspension
and dilution of primers and templates used in the Plexor ® qPCR and qRT-PCR
Systems. This MOPS/EDTA Buffer must be used with the Iso-dC-containing
primers used in the Plexor ® Systems, because these primers are sensitive to
pH below 7.0.
Storage Conditions: Store at any temperature.
For complete and up-to-date product information visit: www.promega.com/catalog
Fluor iso-dC
iso-dC
iso-dGTP
iso-dC
Fluorescence
PCR
product
Quencher
iso-dG
Quencher
several rounds
Threshold
Rounds of PCR
Overview of the core technology in the Plexor ® Real-Time PCR and
RT-PCR Systems.
Monoplex Method of Assaying Multiple Targets
1 Target + 1 Control
Target 1 Control
2 Targets + 1 Control
Target 2
3 Targets + 1 Control
Target 3
Plexor ® Method of Assaying Multiple Targets
1 Target + 1 Control
2 Targets + 1 Control
3 Targets + 1 Control
Comparison of Plexor ® method to monoplex analysis. The multiplexing
power of the Plexor ® System allows you to perform the equivalent of 12 wells
of monoplex analysis in only 3 wells. Productivity is increased and savings are
realized because you use less master mix and less plasticware, and you have
to perform fewer steps.
4775MA
5757MA
211
12
PCR
Section
Contents
Table of
Contents