2012 Promega catalogue

Life
Science
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2012
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Cell Signaling
Real-Time qPCR and RT-qPCR
210
GoTaq ® Real-Time PCR Systems
Product Size Cat.# Price ($)
GoTaq ® qPCR Master Mix 200 reactions A6001 505.00
GoTaq ® 2-Step RT-qPCR System
1,000 reactions A6002 Pls. Enq.
50 RT reactions +
200 qPCR reactions A6010 811.00
GoTaq ® 1-Step RT-qPCR System 200 reactions A6020 867.00
Available Separately
CXR Reference Dye
For Research Use Only. Not for Use in Diagnostic Procedures.
100 µl C5411 36.00
Description: GoTaq ® qPCR Master Mix is a reagent system for use in
real-time quantitative PCR (qPCR and RT-qPCR). The system contains BRYT
Green ® dye, a proprietary fluorescent DNA binding dye that exhibits greater
fluorescence enhancement upon binding to double-stranded DNA (dsDNA)
than SYBR ® Green I. Combined with the GoTaq ® Hot Start Polymerase,
optimized buffer and proprietary dye, GoTaq ® qPCR Master Mix provides
robust real-time PCR with increased reliability, reproducibility and sensitivity.
GoTaq ® qPCR Master Mix is provided as a premixed, ready-to-use stabilized
2X formulation that includes all components for qPCR.
GoTaq ® 2-Step RT-qPCR System is a reagent system for quantitative
analysis of RNA using a two-step reverse transcription-quantitative PCR
(RT-qPCR) protocol. The components and protocol allow robust, reliable
cDNA synthesis of a full range of rare and abundant transcripts, even in the
presence of inhibitors, using the GoScript Reverse Transcription System
and quantification using the GoTaq ® qPCR Master Mix.
GoTaq ® 1-Step RT-qPCR System is a reagent system for quantitative
analysis of RNA using a one-step reverse transcription-quantitative PCR
(RT-qPCR) protocol in a single tube. The BRYT Green ® Fluorescent Dye
and optimized buffer formulations improve data accuracy and sensitivity of
low-expressing targets or targets present at low amounts.
Advantages of the GoTaq ® Real-Time PCR Systems
The BRYT Green ® Fluorescent Dye provides brighter dsDNA-dependent
fluorescence than SYBR ® Green I. With less PCR inhibition than SYBR ®
Green I, the dye enables efficient amplification, often resulting in earlier
quantification cycle (C q) values and an expanded linear range using the same
filters and settings as SYBR ® Green I. The CXR reference dye can be detected
using the same filters and settings as those used for ROX. GoTaq ® qPCR
Master Mix can be used with any real-time instrument capable of detecting
SYBR ® Green I or FAM dye. GoTaq ® qPCR Master Mix contains a low level
of CXR reference dye. If your real-time thermal cycler requires a high level of
reference dye, add CXR Reference Dye to a final concentration of 1X in the
reactions.
Features:
• Brighter Signal: Sensitive detection for earlier quantitation of low- and
high-copy targets.
• Enhanced Stability: Exceptional room-temperature setup makes it
suitable for automation and high-throughput detection.
• Versatile: Compatible with both fast and standard qPCR cycling methods.
• Convenient: Read on any instrument capable of reading SYBR ® Green I
or FAM Dye.
• Flexible: Compatible with reverse transcriptase (RT) of choice or a
complete solution with 1-step and 2-step systems.
• Robust: High-efficiency, full-length cDNA synthesis in the presence of
inhibitors.
• Confidence: The Promega PCR Performance Guarantee.
Storage Conditions: Upon arrival, store all components at –20°C, protected
from light. For immediate use, components may be stored at 2–8°C, protected
from light, for up to 3 months.
Protocol Part#
GoTaq ® qPCR Master Mix Technical Manual
TM318
GoTaq ® 2-Step RT-qPCR System Technical Manual
TM337
GoTaq ® 1-Step RT-qPCR System Technical Manual
TM355
For complete and up-to-date product information visit: www.promega.com/catalog