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2012 Promega catalogue

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Cell Signaling<br />

Deoxynucleotide Triphosphates (dNTPs)<br />

Product Size Conc. Cat.# Price ($)<br />

dATP 25 µmol 100 mM U1205 86.00<br />

40 µmol 100 mM U1201 110.00<br />

200 µmol 100 mM U1202 441.00<br />

dGTP 25 µmol 100 mM U1215 86.00<br />

40 µmol 100 mM U1211 110.00<br />

200 µmol 100 mM U1212 441.00<br />

dCTP 25 µmol 100 mM U1225 86.00<br />

40 µmol 100 mM U1221 110.00<br />

200 µmol 100 mM U1222 441.00<br />

dTTP 25 µmol 100 mM U1235 86.00<br />

40 µmol 100 mM U1231 110.00<br />

200 µmol 100 mM U1232 441.00<br />

Set of dATP, dCTP, dGTP, dTTP 10µmol each 100 mM U1330 110.00<br />

For Laboratory Use.<br />

25 µmol each 100 mM U1420 250.00<br />

40µmol each 100 mM U1240 389.00<br />

200 µmol 100 mM U1410 1351.00<br />

Description:<br />

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success<br />

of many key procedures such as cDNA synthesis, sequencing and labeling.<br />

<strong>Promega</strong> dNTPs are greater than 99% triphosphate content and are provided<br />

at a concentration of 100mM in water at pH 7.5.<br />

Features:<br />

• Dependable: PCR-tested deoxynucleotides ensure optimal performance<br />

with all <strong>Promega</strong> amplification enzymes.<br />

• Consistent: dNTPs are >99% pure, allowing highly consistent results.<br />

• Convenient: Supplied at a convenient concentration (100mM in water)<br />

for ease-of-use in PCR and other applications.<br />

• Choose Your Configuration: Learn more about our custom options for<br />

this product at: www.promega.com/myway/<br />

Storage Conditions: Store at –30°C to –10°C. Avoid exposure to frequent<br />

temperature changes.<br />

PCR Amplifications From Each Size of Individual dNTPs.<br />

Each catalog number supplies each individual dNTP at 100mM. Reactions<br />

are based on 200µM each dNTP in a 50µl reaction.<br />

Cat.# Quantity Volume Reactions<br />

U1330, U1335 10 µmol each 100 µl each 1,000<br />

U1420 25 µmol each 250 µl each 2,500<br />

U1240, U1245 40 µmol each 400 µl each 4,000<br />

U1410 200 µmol each 2 × 1,000 µl each 20,000<br />

9479LA<br />

For complete and up-to-date product information visit: www.promega.com/catalog<br />

Deoxyuridine Triphosphate (dUTP)<br />

Product Size Conc. Cat.# Price ($)<br />

dUTP 40 µmol 100 mM U1191 110.00<br />

Set of dATP, dCTP, dGTP, dUTP 10µmol each 100 mM U1335 110.00<br />

For Laboratory Use.<br />

40µmol each 100 mM U1245 395.00<br />

Description: High-quality deoxynucleotide triphosphates (dNTPs) are critical<br />

for the success of many key procedures such as cDNA synthesis, sequencing<br />

and labeling. <strong>Promega</strong> dNTPs are greater than 98% triphosphate content and<br />

are provided at a concentration of 100mM in water at pH 7.5.<br />

dUTP (2´-Deoxyuridine, 5´-Triphosphate) can be used in place of dTTP in<br />

PCR and RT-PCR protocols to prevent carryover from previous amplifications.<br />

The substitution of dUTP for dTTP in PCR results in uracil-containing PCR<br />

products that are suitable for most standard applications. The enzyme uracil-<br />

N-glycosylase, UNG (also referred to as UDG), can be added to a PCR premix<br />

to excise uracil from any contaminating PCR product, thereby preventing<br />

false positives. Each lot of dUTP is function-tested to ensure specific DNA<br />

amplification and the absence of nuclease activity.<br />

Features:<br />

• Dependable: PCR-tested deoxynucleotides ensure optimal performance<br />

with all <strong>Promega</strong> amplification enzymes.<br />

• Consistent: dUTP is ≥98% pure, allowing highly consistent results.<br />

• Convenient: Supplied at a convenient concentration (100mM in water) for<br />

ease-of-use in PCR and other applications.<br />

Storage Conditions: Store at –20°C. Avoid exposure to frequent temperature<br />

changes.<br />

ART ® Aerosol-Resistant Tips<br />

Product Size Cat.# Price ($)<br />

ART ® 10 Ultramicro Pipet Tip, 0.5–10µl 960 /pk DY1051 265.00<br />

ART ® 20E Ultramicro Pipet Tip, 0.5–10µl 960 /pk DY1061 265.00<br />

ART ® 20P Pipet Tip, 20µl 960 /pk DY1071 265.00<br />

ART ® GEL Gel Loading Pipet Tip, 100µl 960 /pk DY1081 265.00<br />

ART ® 100 Pipet Tip, 100µl 960 /pk DY1101 265.00<br />

ART ® 100E Pipet Tip, 100µl 960 /pk DY1111 265.00<br />

ART ® 200 Pipet Tip, 200µl 960 /pk DY1121 265.00<br />

ART ® 1000E Pipet Tip, 1,000µl<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

800 /pk DY1131 265.00<br />

For additional information see page 14.<br />

209<br />

12<br />

PCR<br />

Section<br />

Contents<br />

Table of<br />

Contents

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