2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
208
Pfu DNA Polymerase
Product Size Conc. Cat.# Price ($)
Pfu DNA Polymerase 100 u 2–3 u/µl M7741 Pls. Enq.
500 u 2–3 u/µl M7745 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available
in all countries. Please contact your local representative for more information.
Description: Pfu DNA Polymerase is a thermostable enzyme of approximately
90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C,
catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´
direction in the presence of magnesium. Pfu DNA Polymerase also possesses
3´→5´ exonuclease (proofreading) activity. Base misinsertions that may occur
during polymerization are rapidly excised by the proofreading activity of the
polymerase. Consequently, Pfu DNA Polymerase is recommended for use in
PCR and primer extension reactions that require high-fidelity synthesis.
Pfu DNA Polymerase-generated PCR fragments are blunt-ended.
Pfu DNA Polymerase 10X Reaction Buffer with MgSO 4: 200mM Tris-HCl
(pH 8.8 at 25°C), 100mM KCl, 100mM (NH 4) 2SO 4, 20mM MgSO 4,
1.0% Triton ® X-100 and 1mg/ml nuclease-free BSA.
Features:
• High Fidelity: Pfu DNA Polymerase exhibits the lowest error rate of any
thermostable DNA polymerase.
• Complete: Provided with 10X Buffer containing 20mM MgSO 4.
• Performance Guarantee: Promega PCR systems, enzymes and reagents
are proven in PCR to ensure reliable, high-performance results. If you are
not completely satisfied with any Promega PCR product, we will send a
replacement or refund your account.
Storage Conditions: Store at –20°C.
Protocol Part#
Pfu DNA Polymerase Protocol 9PIM774
PCR Nucleotide Mix
Product Size Conc. Cat.# Price ($)
PCR Nucleotide Mix 200 µl 10 mM C1141 111.00
For Laboratory Use.
1,000 µl 10 mM C1145 412.00
Description: High-quality deoxynucleotide triphosphates (dNTPs) are critical
for the success of the polymerase chain reaction (PCR). The PCR Nucleotide
Mix is a premixed solution containing the sodium salts of dATP, dCTP, dGTP
and dTTP, each at a concentration of 10mM in water; the total concentration
of nucleotides, therefore, is 40mM (pH 7.5). This solution is ready to use and is
optimized for standard polymerase chain reactions and specialty approaches
including hot-start and reverse transcription PCR (RT-PCR). One microliter (1μl)
is sufficient for PCR amplification in a typical 50μl reaction volume.
Features:
• Optimized and Pretested in PCR: Equimolar amounts of each dNTP
ensure optimal PCR.
• Convenient: 1μl addition for 50μl PCR.
• Easy to Use: Reduced pipetting steps contribute to ease-of-use and
reduce the risk of contamination.
• Performance Guarantee: Promega PCR systems, enzymes and reagents
are proven in PCR to ensure reliable, high-performance results. If you are
not completely satisfied with any Promega PCR product, we will send a
replacement or refund your account.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C. Avoid exposure to frequent temperature
changes.
Protocol Part#
PCR Nucleotide Mix Protocol 9PIC114
dNTP Mix
Product Size Conc. Cat.# Price ($)
dNTP Mix 200 µl 10 mM U1511 41.00
For Laboratory Use.
1,000 µl 10 mM U1515 98.00
Description: dNTP Mix is a premixed solution containing sodium salts of
dATP, dCTP, dGTP and dTTP, each at 10mM in water; the total concentration of
nucleotides, therefore, is 40mM (pH 7.5). One microliter of the dNTP Mix into a
50μl reaction will give a final dNTP concentration of 200μM for each dNTP.
Features:
• High Purity: dNTPs are >98% pure.
• Easy to Use: Reduced pipetting steps contribute to ease-of-use and
reduce the risk of contamination.
Storage Conditions: Store at –20°C. Avoid exposure to frequent temperature
changes.
For complete and up-to-date product information visit: www.promega.com/catalog