2012 Promega catalogue

Cell Signaling
GoTaq ® PCR Core Systems
Product Size Cat.# Price ($)
GoTaq ® PCR Core System I 200 reactions M7660 318.00
GoTaq ® PCR Core System II 200 reactions M7665 345.00
M7660, M7665 For Laboratory Use.
Description: The GoTaq ® PCR Core Systems I and II are designed for the
exponential amplification of specific regions of DNA using the polymerase
chain reaction. Both systems include GoTaq ® DNA polymerase and PCR
Nucleotide Mix, along with high-performance buffers and magnesium chloride.
The GoTaq ® PCR Core System II also includes a Positive Control Plasmid DNA
and Positive Control Primers, providing increased confidence in PCR control
reactions. Each system’s components are performance-tested in PCR and are
sufficient for 200 reactions. The GoTaq ® PCR Core Systems are provided
with a Technical Bulletin that provides simple protocols and a complete
troubleshooting guide.
Features:
• Convenient: PCR-tested components are provided in optimized volumes
for 200 reactions.
• Flexible: Optimization tools are provided for reaction flexibility.
• Positive Controls: The GoTaq ® PCR Core System II provides Positive
Control Plasmid DNA and Control Primers to help troubleshoot PCR
parameters.
• Performance Guarantee: Promega PCR systems, enzymes and reagents
are proven in PCR to ensure reliable, high-performance results. If you are
not completely satisfied with any Promega PCR product, we will send a
replacement or refund your account.
Storage Conditions: Store all components at –20°C.
Protocol Part#
GoTaq ® PCR Core Systems Technical Bulletin
TB254
PCR Master Mix
Product Size Conc. Cat.# Price ($)
PCR Master Mix 10 reactions 2 X M7501 21.00
100 reactions 2 X M7502 108.00
For Laboratory Use.
1,000 reactions 2 X M7505 783.00
Description: PCR Master Mix is a premixed, ready-to-use solution containing
Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations
for efficient amplification of DNA templates by PCR. The PCR Master Mix
has been optimized for use in routine PCR for amplifying DNA templates in the
range of 0.2–2kb.
Features:
• Fast: Set up reactions in less than a minute.
• Sensitive: Amplify as little as 2 copies of target template.
• Convenient: One tube, one pipetting step.
• Complete: Reagents, including Taq DNA polymerase, MgCl 2, dNTPs and
buffers, in one tube.
• Scalable: Set up 10μl, 25μl or 50μl reactions.
• Stable: Store at 4°C for up to 3 months.
• Performance Guarantee: Promega PCR systems, enzymes and reagents
are proven in PCR to ensure reliable, high-performance results. If you are
not completely satisfied with any Promega PCR product, we will send a
replacement or refund your account.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C. PCR Master Mix can be stored at 4°C
for up to 3 months.
Protocol Part#
PCR Master Mix Protocol 9PIM750
For complete and up-to-date product information visit: www.promega.com/catalog
Template Copies per Reaction
M 0 2 10 100 1,000 M
Detection of low-copy-number templates using PCR Master Mix. Use
of PCR Master Mix to detect low number of copies of the α-1-antitrypsin
gene. PCR was performed on Human Genomic DNA (Cat.# G3041) using
primers targeting a 360bp fragment of the α-1-antitrypsin gene (single copy
per genome). Lane M, 100bp DNA Ladder (Cat.# G2101).
Tfl DNA Polymerase
Product Size Conc. Cat.# Price ($)
Tfl DNA Polymerase 100 u 5 u/µl M1941 105.00
For Laboratory Use.
1,000 u 5 u/µl M1945 666.00
Description: Tfl DNA Polymerase is a thermostable enzyme of approximately
94kDa isolated from Thermus flavus. The enzyme replicates DNA at 74°C and
exhibits a half-life of 40 minutes at 95°C. Tfl DNA Polymerase catalyzes the
polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the
presence of magnesium and the polymerization of nucleotides into DNA using
an RNA template in the 5´→3´ direction in the presence of manganese. The
enzyme also possesses a 5´→3´ exonuclease activity. Tfl DNA Polymerase
is recommended for use in PCR and primer extension reactions at elevated
temperatures.
Tfl DNA Polymerase 10X Reaction Buffer: 200mM Tris-acetate
(pH 8.9 at 25°C), 100mM ammonium sulfate.
Magnesium Sulfate: 25mM MgSO 4 Solution included.
Features:
• Flexible: Provided with a 10X Reaction Buffer that does not contain
magnesium. Sufficient 25mM MgSO 4 is provided separately to allow
optimization of enzyme performance under different conditions.
• Performance Guarantee: Promega PCR systems, enzymes and reagents
are proven in PCR to ensure reliable, high-performance results. If you are
not completely satisfied with any Promega PCR product, we will send a
replacement or refund your account.
Storage Conditions: Store at –20°C.
3063TA09_0A
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12
PCR
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