2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Routine PCR
206
GoTaq ® Amplification Family
Product Size Conc. Cat.# Price ($)
GoTaq ® Flexi DNA Polymerase 100 u 5 u/µl M8291 101.00
500 u 5 u/µl M8295 393.00
2,500 u 5 u/µl M8296 1528.00
5,000 u 5 u/µl M8297 Pls. Enq.
10,000 u 5 u/µl M8298 Pls. Enq.
GoTaq ® DNA Polymerase 100 u 5 u/µl M3001 101.00
500 u 5 u/µl M3005 393.00
2,500 u 5 u/µl M3008 1528.00
GoTaq ® Green Master Mix 100 reactions M7122 108.00
1,000 reactions M7123 783.00
GoTaq ® Colorless Master Mix 100 reactions M7132 108.00
For Laboratory Use.
1,000 reactions M7133 783.00
Description: Experience improved PCR performance with GoTaq ® DNA
Polymerase products. GoTaq ® DNA Polymerase is a proprietary formulation of
Taq DNA polymerase that gives robust amplification equal to and in some cases
superior to that of standard Taq DNA polymerase. GoTaq ® DNA Polymerase
comes in a variety of formulations designed to give you maximum flexibility,
control and convenience.
GoTaq ® Flexi DNA Polymerase allows you to optimize enzyme and
magnesium concentration in your PCR. The supplied 5X Green and Colorless
Flexi Reaction Buffers do not contain magnesium. A separate tube of
25mM MgCl 2 is supplied, giving you maximum control over your reaction
conditions. MgCl 2 is also available separately.
GoTaq ® DNA Polymerase provides improved amplification with the
convenience of reaction buffers containing magnesium. The 5X GoTaq ®
Green and Colorless Reaction Buffers supplied with GoTaq ® DNA Polymerase
contain MgCl 2 at a concentration of 7.5mM, giving a final concentration of
1.5mM in the 1X reaction.
The 5X Green and 5X Colorless Reaction Buffers supplied with GoTaq ®
enzymes allow you to go directly from thermal cycler to gel analysis. These
buffers contain a compound that increases sample density, so that samples
sink easily into the wells of an agarose gel. The green buffer also contains
two dyes (yellow and blue) that separate to allow easy monitoring during
electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA
fragments in a 1% agarose gel, and the yellow dye migrates ahead of primers.
For ultimate convenience, choose GoTaq ® Colorless Master Mix or
GoTaq ® Green Master Mix. Both are premixed, ready-to-use 2X solutions
containing GoTaq ® DNA Polymerase, dNTPs, MgCl 2 and reaction buffers at
optimal concentrations for efficient amplification of DNA templates by PCR.
GoTaq ® Green Master Mix also includes two dyes (blue and yellow) that allow
monitoring of progress during electrophoresis. GoTaq ® Colorless Master Mix has
the same formulation as the GoTaq ® Green Master Mix but does not include the
dyes. Both also include Nuclease-Free Water. Reactions assembled with GoTaq ®
Master Mixes have sufficient density for direct loading onto agarose gels.
Features:
• Improve Performance: Experience better PCR performance with this new
buffer and enzyme formulation. With GoTaq ® Flexi, you also have the option
to titrate Mg 2+ concentration in your reactions.
• Improve Productivity: Go directly from PCR to gel analysis. Green GoTaq ®
Reaction Buffer serves as both reaction buffer and gel-loading solution.
• Keep Your Cycling Conditions: Directly substitute GoTaq ® products,
with either Colorless or Green Reaction Buffer, in your current PCR
application—no need to change cycling parameters.
• Use With PCR Enhancers: GoTaq ® DNA Polymerase is compatible with
PCR enhancers such as betaine and DMSO. Neither compound affects the
color or characteristics of the GoTaq ® Green Reaction Buffer.
• Fast and Convenient: GoTaq ® Green Master Mix offers the ultimate in
convenience. Reactions can be set up in less than a minute; simply add
your template, water and primers and go!
Storage Conditions: Store enzymes at –20°C. GoTaq ® Green Master Mix
can be stored at 4°C for 6 weeks.
Protocol Part#
GoTaq ® Flexi DNA Polymerase (M829) Protocol
9PIM829
GoTaq ® DNA Polymerase (M300) Protocol 9PIM300
GoTaq ® Green Master Mix (M712) Protocol
9PIM712
GoTaq ® Colorless Master Mix (M713) Protocol
9PIM713
1.2kb–
10 3
copies
10
M G N T A G N T A G N T A
2 101 GoTaq ® Green Master Mix outperforms standard Taq DNA Polymerase.
Amplification of a 1.2kb fragment of the α-1-antitrypsin gene from indicated
copies of Human Genomic DNA (Cat.# G3041). Lanes G, GoTaq ® Green
Master Mix; Lanes N, competitor N; Lanes T, competitor T; Lanes A,
competitor A; and Lane M, BenchTop 1kb DNA Ladder (Cat.# G7541).
GoTaq ® Reaction Buffers and Magnesium
Chloride
Product Size Conc. Cat.# Price ($)
5X Green GoTaq ® Reaction Buffer 20 ml M7911 54.00
5X Colorless GoTaq ® Reaction Buffer 20 ml M7921 54.00
5X Colorless GoTaq ® Flexi Reaction Buffer 20 ml M8901 54.00
5X Green GoTaq ® Flexi Reaction Buffer 20 ml M8911 54.00
Magnesium Chloride Solution 1.5 ml 25 mM A3511 46.00
For Laboratory Use.
25 ml 25 mM A3513 91.00
Description: The 5X Green GoTaq ® Reaction Buffer contains two dyes
(a blue dye and a yellow dye) that separate during electrophoresis to show
migration progress. The buffer also contains a compound that increases
sample density. This means that samples can be loaded directly onto gels
without the need for loading dye. The blue dye migrates at the same rate as a
3–5kb DNA fragment in a 1% agarose gel. The yellow dye migrates at a rate
faster than primers (