2012 Promega catalogue

Cell Signaling
TaqBead Hot Start Polymerase
Product Size Cat.# Price ($)
TaqBead Hot Start Polymerase, 1.25u/
bead, Nonbarrier 100 reactions M5661 147.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: TaqBead Hot Start Polymerase consists of spherical beads
of wax containing Taq DNA polymerase. TaqBead Hot Start Polymerase
facilitates hot-start PCR by keeping the enzyme sequestered in paraffin wax
until the reaction temperature reaches approximately 60°C. This increases PCR
specificity by keeping the polymerase separate from the rest of the reaction
components until a critical temperature is reached, decreasing the probability
of amplifying products that are the result of nonspecific binding of primers.
The nonbarrier format of the bead is intended for use with heated-lid thermal
cyclers or with the addition of a mineral oil overlay.
Thermophilic DNA Polymerase 10X Reaction Buffer: 500mM KCl,
100mM Tris-HCl (pH 9.0) at 25°C, 1.0% Triton ® X-100.
Magnesium Chloride: 25mM MgCl 2 Solution included.
Features:
• Higher Yield PCR: Hot-start PCR protocols increase amplimer yield by
minimizing nonspecific priming, primer-dimer formation or other reactions
that can occur at low temperatures once all the PCR amplification
components are mixed.
• Increased Specificity: Hot-start format reduces the incidence of
nonspecific amplification products.
• Reliable: Minimized nonspecific priming results in increased
reproducibility of amplification reactions.
• Flexible: Sufficient 25mM MgCl 2 is provided separately to allow
optimization of enzyme performance under different conditions.
Storage Conditions: Store at –20°C.
Protocol Part#
TaqBead Hot Start Polymerase Technical Bulletin TB247
Long PCR
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GoTaq ® Long PCR Master Mix
Product Size Cat.# Price ($)
GoTaq ® Long PCR Master Mix 100 reactions M4021 393.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: GoTaq ® Long PCR Master Mix contains the high-performance
GoTaq ® Hot Start Polymerase in a specially formulated mixture with a
proprietary thermal stable proofreading polymerase. This optimized enzyme
mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb
from human genomic DNA. The presence of a proofreading enzyme to repair
DNA mismatches in the presence of a highly processive polymerase allows the
polymerase to continue to elongate the DNA much further, resulting in longer
DNA amplification.
The optimized formulation of the GoTaq ® Long PCR Master Mix components
have been engineered to enable simple reaction setup and provide consistently
efficient, accurate and robust performance of long DNA amplicons.
Features:
The proven robust amplification of GoTaq ® Polymerase is now available for
long-range PCR (up to 30kb gDNA).
• Easy: Hot-start master mix for convenient handling and simple setup.
• Enhanced: Yield, sensitivity and specificity with optimized components.
• Accurate: Blend of thermostable DNA polymerases with enhanced
processivity and proofreading.
• Confidence: Control primer pair and human gDNA template to control
reactions and test template quality.
• Efficient: Perfect for cloning genes, mutational analysis and DNA
sequencing.
Storage Conditions: Upon arrival, store all components at –20°C, protected
from light. For immediate use, components may be stored at 2–8°C, protected
from light, for up to 3 months.
Protocol Part#
GoTaq ® Long PCR Master Mix Technical Manual
TM359
205
12
PCR
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