2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Hot-Start PCR
204
GoTaq ® Hot Start Polymerase
Product Size Cat.# Price ($)
GoTaq ® Hot Start Polymerase 100 u M5001 121.00
500 u M5005 472.00
2,500 u M5006 1833.00
10,000 u M5008 Pls. Enq.
GoTaq ® Hot Start Green Master Mix 100 reactions M5122 159.00
1,000 reactions M5123 1261.00
GoTaq ® Hot Start Colorless Master Mix 100 reactions M5132 159.00
For Research Use Only. Not for Use in Diagnostic Procedures.
1,000 reactions M5133 1261.00
Description: GoTaq ® Hot Start Polymerase contains the high-performance
GoTaq ® DNA Polymerase bound to a proprietary antibody that blocks
polymerase activity. The polymerase activity is restored during the initial
denaturation step when the amplification reactions are heated at 94–95°C
for two minutes. This allows for hot-start PCR, where polymerase activity is
eliminated or minimized at temperatures below 70°C. GoTaq ® Hot Start
Polymerase exhibits 5´→3´ exonuclease activity. The system is supplied with
a tube of 25mM MgCl 2, allowing optimization of the magnesium concentration
in your reactions. It is also supplied with 5X Green GoTaq ® Flexi Buffer and
5X Colorless GoTaq ® Flexi Buffer. The buffers contain a compound that
increases sample density, so that samples sink easily into the wells of an
agarose gel. The green buffer also contains two dyes (yellow and blue) that
separate to allow easy monitoring during electrophoresis. Use the green
reaction buffer for direct-to-gel analysis after amplification and the colorless
reaction buffer for amplifications where the dyes may interfere with postamplification
analysis such as fluorescence or absorbance testing.
GoTaq ® Hot Start Master Mixes are premixed, ready-to-use solutions containing
GoTaq ® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can
be set up in less than a minute at room temperature; simply add your template,
water and primers. Available with either green or colorless reaction buffers,
which also serve as loading buffers, allowing you to go directly from thermal
cycler to gel analysis. GoTaq ® Hot Start Master Mixes offer the specificity
and sensitivity of an antibody-based hot-start polymerase in a convenient,
easy-to-use, time-saving format.
Features:
• Enhance Yield, Sensitivity and Specificity: The proven, robust
amplification and sensitivity of GoTaq ® DNA Polymerase now with a
built-in hot start to deliver even more superior results.
• Ease of Use: Set up your reaction at room temperature—no need to
set up on ice.
• Higher Yield: Two-minute activation saves time and ensures maximum
enzyme activity.
• Higher Specificity: Minimize nonspecific amplification and primer-dimers.
• Improve Productivity: Go directly from PCR to gel analysis. Green
GoTaq ® Reaction Buffer serves as both reaction buffer and gel-loading
solution.
• Convenient: One tube, one pipetting step. Only add template and primers
when using the master mixes.
• Optimization: Control the magnesium in your reaction for specialized
templates when using the standalone polymerase.
Storage Conditions: Store at –20°C.
Protocol Part#
GoTaq ® Hot Start Polymerase Protocol
9PIM500
GoTaq ® Hot Start Green Master Mix Protocol
9PIM512
GoTaq ® Hot Start Colorless Master Mix Protocol
9PIM513
bp
1,605
1,198
517
350
Standard
Taq
GoTaq ®
Hot Start
M M
Colorless
Green
Colorless
Green
Improve amplification of targets that require hot start using GoTaq ®
Hot Start Polymerase. A 1.5kb fragment of a Corynephage omega gene that
requires hot start PCR was amplified from 500pg of plasmid DNA using either
standard Taq or GoTaq ® Hot Start Polymerase in Green and Colorless Flexi
Reaction Buffers. Use of GoTaq ® Hot Start Polymerase resulted in amplification
of only the target 1.5kb fragment. Using standard Taq DNA Polymerase, a
nonspecific 410bp product also was amplified.
For complete and up-to-date product information visit: www.promega.com/catalog
6988TA