2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
202<br />
Y Chromosome Deletion Detection System,<br />
Version 2.0<br />
Product Size Cat.# Price ($)<br />
Y Chromosome Deletion Detection System,<br />
Version 2.0 25 reactions MD1531 Pls. Enq.<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The Y Chromosome Deletion Detection System, Version 2.0,<br />
provides a standardized screening panel amplifying only informative nonpolymorphic<br />
sequence tag sites (STS) on the human Y chromosome. The system<br />
amplifies key functional regions associated with AZoospermia Factor (AZF),<br />
namely the regions that flank AZFa and cover AZFb, AZFc, AZFd including DAZ,<br />
KAL-Y, SMCY and flanking loci for other key spermatogenesis-related genes<br />
(namely RBM1, DFFRY and DBY).<br />
Five Multiplex Master Mixes, with a total of 20 characterized Y-specific primer<br />
pairs, are included. Four of the multiplex primer sets contain a control primer<br />
pair that amplifies a fragment of the X-linked SMCX locus. One of the multiplex<br />
primer sets (Multiplex E Master Mix) contains a control primer pair that amplifies<br />
a unique region in both male and female DNA (ZFX/ZFY). Finally, a primer<br />
pair that amplifies a region of the SRY gene has been included in Multiplex E<br />
Master Mix as a control for the testis-determining factor on the short arm of the<br />
Y chromosome to detect XX males arising from Y to X translocations.<br />
The Multiplex Master Mixes are designed to facilitate the simultaneous amplification<br />
of several different regions of the Y chromosome. The amplification<br />
products (83–496bp) of the five multiplex PCR amplifications can be clearly<br />
separated by agarose gel electrophoresis and visualized by ethidium bromide<br />
staining.<br />
Failure to amplify specific regions of the Y chromosome is indicative of Y<br />
chromosome deletions in the test sample. The size control ladder provided<br />
minimizes analysis time and the possibility of misinterpreting molecular weight<br />
of amplification products.<br />
Features:<br />
• Ease of Use: Premixed Multiplex Master Mixes contain 20 primer pairs,<br />
including internal controls providing a standardized panel of results requiring<br />
no user optimization.<br />
• More Robust Reactions: Improved formulation and use of GoTaq ® DNA<br />
Polymerase minimizes dropouts.<br />
• Flexibility: Amplify genomic DNA purified using various methods and with<br />
a PE480 (oil overlay) or PE9600/9700 (non-oil overlay) thermal cycler.<br />
• Complete System: All required reagents are provided in the kit.<br />
Storage Conditions: Store at –20°C.<br />
Protocol Part#<br />
Y Chromosome Deletion Detection System, Version 2.0 Technical<br />
Manual<br />
TM248<br />
Primer Sets in the Y Chromosome Deletion Detection System.<br />
Locus/ Locus/ Locus/ Locus/ Locus/<br />
Multiplex STS 1 STS 2 STS 3 STS 4 STS 5<br />
Master Mix A DAZ/ DYS240/ DYS271/ DYS221/ KAL-Y/<br />
SY254 SY157 SY81 SY130 SY182<br />
Master Mix B SMCY/ DYS218/ DAZ/ DAZ/<br />
SYPR3 SY127 SY242 SY208<br />
Master Mix C DYS219/ DYS212/ DYF51S1/ DAZ/<br />
SY128 SY121 SY145 SY255<br />
Master Mix D DYS236/ DYS223/ DYS215/<br />
SY152 SY133 SY124<br />
Master Mix E SRY/ DYS224/ DYS148/ DYS273/ ZFX1/<br />
SY14 SY134 SY86 SY84 ZFY 9492LA<br />
For complete and up-to-date product information visit: www.promega.com/catalog
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