2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Reagents for Molecular
Diagnostics Labs
200
GoTaq ® MDx Hot Start Polymerase
Product Size Cat.# Price ($)
GoTaq ® MDx Hot Start Polymerase 100 u D6001 125.00
500 u D6005 Pls. Enq.
2,500 u D6006 Pls. Enq.
For Laboratory Use.
10,000 u D6008 Pls. Enq.
Description: GoTaq ® MDx Hot Start Polymerase contains the high-performance
GoTaq ® DNA Polymerase bound to a proprietary antibody that blocks
polymerase activity. The polymerase activity is restored during the initial
denaturation step when the amplification reactions are heated at 94–95°C
for two minutes. The system is supplied with a tube of 25mM MgCl 2, allowing
optimization of the magnesium concentration in your reactions. It is also
supplied with 5X Green GoTaq ® Flexi Buffer and 5X Colorless GoTaq ® Flexi
Buffer. The buffers contain a compound that increases sample density, so that
samples sink easily into the wells of an agarose gel. The green buffer also
contains two dyes (yellow and blue) that separate to allow easy monitoring
during electrophoresis. Use the green reaction buffer for direct-to-gel analysis
after amplification and the colorless reaction buffer for amplifications where
the dyes may interfere with post-amplification analysis such as fluorescence or
absorbance testing.
GoTaq ® MDx Hot Start Polymerase is a general purpose reagent that can be
used for clinical applications or as a component of molecular diagnostic assays
without paying royalties. The products by themselves do not provide any
diagnostic result.
Features:
The proven robust amplification of GoTaq ® MDx Hot Start Polymerase is now
available for use in diagnostics and clinical applications:
• Easy: Convenient handling with room temperature setup.
• Fast: Results with only two-minute enzyme activation.
• Enhanced: Yield, sensitivity and specificity with an antibody hot start.
• Ready: High-quality product developed for integration into Laboratory
Developed Tests and homebrew assays.
To use GoTaq ® MDx Hot Start Polymerase in a custom format for diagnostic
assays or to distribute GoTaq ® MDx Hot Start Polymerase, please contact the
Promega Custom Order Department to discuss specific requirements.
Storage Conditions: Store at –20°C.
Protocol Part#
GoTaq ® MDx Hot Start Polymerase Product Information
9PID600
GoTaq ® Amplification Family
Product Size Conc. Cat.# Price ($)
GoTaq ® Flexi DNA Polymerase 100 u 5 u/µl M8291 101.00
500 u 5 u/µl M8295 393.00
2,500 u 5 u/µl M8296 1528.00
5,000 u 5 u/µl M8297 Pls. Enq.
10,000 u 5 u/µl M8298 Pls. Enq.
GoTaq ® DNA Polymerase 100 u 5 u/µl M3001 101.00
500 u 5 u/µl M3005 393.00
2,500 u 5 u/µl M3008 1528.00
GoTaq ® Green Master Mix 100 reactions M7122 108.00
1,000 reactions M7123 783.00
GoTaq ® Colorless Master Mix 100 reactions M7132 108.00
1,000 reactions M7133 783.00
M8291, M8295, M8296, M8297, M8298, M3001, M3005, M3008, M7122, M7123, M7132,
M7133 For Laboratory Use.
For additional information see page 206.
GoScript Reverse Transcriptase
Product Size Cat.# Price ($)
GoScript Reverse Transcriptase 100 reactions A5003 388.00
For Laboratory Use.
500 reactions A5004 1450.00
Description: GoScript Reverse Transcriptase utilizes M-MLV and state-ofthe-art
buffer technology designed for qPCR to deliver robust, reliable cDNA
synthesis of a full range of rare and abundant transcripts, even in the presence
of inhibitors. GoScript Reverse Transcriptase is qualified for use in qPCR,
including GoTaq ® qPCR and Plexor ® qPCR systems for performing RT-qPCR.
Features:
• Ultra-Active: Save money on every reaction.
• Sensitive: Detect rare transcripts.
• Processive: Transcribe long messages.
• Resilient: Synthesize cDNA in the presence of strong inhibitors.
• Replaces Superscript ® I, II, III and other RTs.
Storage Conditions: Store at –20°C.
GoScript Reverse Transcription System
Product Size Cat.# Price ($)
GoScript Reverse Transcription System 50 reactions A5000 450.00
For Laboratory Use.
For additional information see page 213.
100 reactions A5001 800.00
Deoxynucleotide Triphosphates (dNTPs)
Product Size Conc. Cat.# Price ($)
dATP 25 µmol 100 mM U1205 86.00
40 µmol 100 mM U1201 110.00
200 µmol 100 mM U1202 441.00
dGTP 25 µmol 100 mM U1215 86.00
40 µmol 100 mM U1211 110.00
200 µmol 100 mM U1212 441.00
dCTP 25 µmol 100 mM U1225 86.00
40 µmol 100 mM U1221 110.00
200 µmol 100 mM U1222 441.00
dTTP 25 µmol 100 mM U1235 86.00
40 µmol 100 mM U1231 110.00
200 µmol 100 mM U1232 441.00
Set of dATP, dCTP, dGTP, dTTP 10µmol each 100 mM U1330 110.00
For Laboratory Use.
25 µmol each 100 mM U1420 250.00
40µmol each 100 mM U1240 389.00
200 µmol 100 mM U1410 1351.00
Description:
High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success
of many key procedures such as cDNA synthesis, sequencing and labeling.
Promega dNTPs are greater than 99% triphosphate content and are provided
at a concentration of 100mM in water at pH 7.5.
Storage Conditions:
Store at –30°C to –10°C.
For complete and up-to-date product information visit: www.promega.com/catalog