2012 Promega catalogue

Life
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2012
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Cell Signaling
Maxwell ® 16 System RNA Purification Kits
Product Size Cat.# Price ($)
Maxwell ® 16 LEV simplyRNA Cells Kit 48 preps AS1270 Pls. Enq.
Maxwell ® 16 LEV simplyRNA Tissue Kit 48 preps AS1280 Pls. Enq.
Maxwell ® 16 Total RNA Purification Kit 48 preps AS1050 Pls. Enq.
Maxwell ® 16 Tissue LEV Total RNA Purification
Kit 48 preps AS1220 Pls. Enq.
Maxwell ® 16 Cell LEV Total RNA Purification Kit 48 preps AS1225 Pls. Enq.
Maxwell ® 16 Viral Total Nucleic Acid
Purification Kit 48 preps AS1150 Pls. Enq.
Available Separately
Maxwell ® 16 High Strength LEV Magnetic Rod
and Plunger Bar Adaptor 1 each SP1070 Pls. Enq.
AS1270, AS1280, AS1050, AS1220, AS1225, AS1150 For Laboratory Use. SP1070 For
Research Use Only. Not for Use in Diagnostic Procedures.
Description:
The Maxwell ® 16 LEV simplyRNA Cells Kit and the Maxwell ® 16 LEV simplyR-
NA Tissue Kit are for use with the Maxwell ® 16 Instrument configured with the
LEV High Strength Magnetic Rod and Plunger Bar Adaptor. This RNA purification
procedure is a simple method with minimal lysate handling before automated
purification on the Maxwell ® 16 Instrument. The low elution volume is used
to generate concentrated high-quality RNA suitable for use in downstream
applications such as quantitative RT-PCR. The kit provides the reagents for
processing the samples and uses prefilled cartridges for purification, maximizing
simplicity and convenience.
The Maxwell ® 16 Total RNA Purification Kit, Maxwell ® 16 Tissue LEV Total
RNA Purification Kit and Maxwell ® 16 Cell LEV Total RNA Purification Kit are
designed for use with the Maxwell ® 16 Instrument in either the standard or low
elution volume (LEV) configuration. The kits provide high-quality,
essentially DNA-free total RNA using novel approaches to selectively remove
genomic DNA prior to automated RNA purification. You get enhanced sensitivity
and improved confidence in your results for quantitative RT-PCR (qRT-PCR),
RT-PCR, cDNA synthesis and other applications.
The simple protocols require adding a cleared lysate to the reagent cartridge.
Simply place the reagent cartridge into the Maxwell ® 16 Instrument, and press
start. Purified RNA is obtained in less than 45 minutes of hands-free instrument
operation. No post-purification treatment with nuclease, cleanup or concentration
is required to achieve superior performance in downstream applications.
The Maxwell ® 16 Total RNA Purification Kits are General Purpose Medical
Devices (GPR) in the USA. Visit: www.promega.com/maxwell16/ for up-todate
information.
Features:
• Enjoy Confidence in Your Application Results: Essentially undetectable
contaminating genomic DNA means fewer repeated experiments and
unexplained or variable results.
• Choose Your Sample Type: Flexibility to purify from tissue, cells, blood
and other samples.
• Achieve High Yield and High Concentration: High yields and highconcentration
total RNA result in better performance in gene expression
analysis applications.
198
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
No detectable cross-contamination. Sixteen purification reactions were
performed using an input of 25mg of mouse liver lysate (odd lanes) or SV
RNA Lysis Buffer alone (even lanes). Panel A. Four-microliter aliquots of
each purified sample were resolved by 1.2% agarose gel electrophoresis
under denaturing conditions. Lane M, RNA Markers (Cat.# G3191). Panel B.
Equivalent volumes (1μl) of each sample were amplified by endpoint RT-PCR
using a primer pair specific for a portion of beta actin RNA. A total of five
microliters of each amplification reaction was analyzed by 1.2% agarose gel
electrophoresis and visualized by ethidium bromide staining. Lane M, 1kb DNA
Ladder (Cat.# G5711).
TISSUE
simply RNA
kidney brain heart lung spleen liver HEK293 HeLa Jurkat
For complete and up-to-date product information visit: www.promega.com/catalog
CELLS
10001TA
5967TB
28S
18S
Intact RNA Extracted from Tissue Using the Maxwell ® 16 LEV simplyRNA Tissue
Kit. Extracted tissue samples were run on a FlashGel ® System for 5 minutes and signal
developed for 15 minutes. The 28S, 18S and 5S are clearly visible indicating intact RNA.
5S