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2012 Promega catalogue

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Life<br />

Science<br />

Catalog<br />

<strong>2012</strong><br />

Worldwide Contact List<br />

Section<br />

Contents<br />

Table of<br />

Contents<br />

Cell Signaling<br />

DNA Purification from Food<br />

180<br />

Wizard ® Magnetic DNA Purification System<br />

for Food<br />

Product Size Cat.# Price ($)<br />

Wizard ® Magnetic DNA Purification System<br />

for Food<br />

200 preps FF3750 1336.00<br />

400 preps FF3751 2249.00<br />

Available Separately<br />

Lysis Buffer A, Food 100 ml A8191 117.00<br />

Lysis Buffer B, Food 100 ml Z3191 345.00<br />

Precipitation Solution, Food 150 ml Z3201 705.00<br />

A8191, Z3191, Z3201 For Research Use Only. Not for Use in Diagnostic Procedures.<br />

Description: The Wizard ® Magnetic DNA Purification System for Food purifies<br />

DNA from a variety of food samples including corn seeds, cornmeal, soybeans,<br />

soy flour and soy milk. Processed food, such as corn chips, chocolate and<br />

chocolate-containing foods, lecithin and vegetable oils may also be used with<br />

the suggested protocol variations. The purified DNA can be used in PCR-based<br />

testing for genetically modified organism (GMO) DNA sequences.<br />

Features:<br />

• Improved Productivity: Obtain results in one-third the time of current<br />

methods.<br />

• Ease of Handling: Requires minimal centrifugation and eliminates organic<br />

extractions.<br />

• Versatility and Robustness: Validated with a broad variety of foodstuffs,<br />

including difficult samples such as lecithin and vegetable oils.<br />

Storage Conditions: Store at 22–25°C.<br />

Protocol Part#<br />

Wizard ® Magnetic DNA Purification System for Food Technical<br />

Bulletin<br />

TB284<br />

Wizard ® Magnetic 96 DNA Plant System<br />

Product Size Cat.# Price ($)<br />

Wizard ® Magnetic 96 DNA Plant System 2 × 96 preps FF3760 579.00<br />

Available Separately<br />

4 × 96 preps FF3761 1121.00<br />

Wash Buffer, Plant<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

40 ml A3811 208.00<br />

Description: The Wizard ® Magnetic 96 DNA Plant System is designed for<br />

manual or automated 96-well, high-throughput purification of DNA from plant<br />

leaf and seed tissue. The system has been validated with corn and tomato leaf,<br />

as well as with canola and sunflower seeds. The purified DNA can be used in<br />

PCR and applications such as RAPD analysis. Unlike column-based systems,<br />

the binding of nucleic acids to magnetic particles can occur in solution,<br />

enhancing contact with the wash buffer and increasing nucleic acid purity.<br />

Protocols are available for Beckman Coulter instruments.<br />

Features:<br />

• Improved Productivity: Manual and automated 96-well protocols cut<br />

purification time compared to CTAB extraction.<br />

• Ease of Handling: Eliminates organic extractions, multiple centrifugations<br />

and cumbersome filter plates.<br />

• Confidence in Applications Performance: Validated for both leaf and<br />

seed tissue by PCR and RAPD analysis.<br />

• Automation: Validated automated methods available at:<br />

www.promega.com/automethods/<br />

• Your Choice of Configuration: Learn more about our custom options for<br />

this product at: www.promega.com/myway/<br />

Storage Conditions: Store at 22–25°C.<br />

Protocol Part#<br />

Wizard ® Magnetic 96 DNA Plant System Technical Bulletin TB289<br />

ENLITEN ® ATP Assay System<br />

Product Size Cat.# Price ($)<br />

ENLITEN ® ATP Assay System 100 assays FF2000 339.00<br />

Description: The ENLITEN ® ATP Assay System can be used to measure<br />

ATP levels for the indirect detection of biocontamination on food processing<br />

surfaces, in cosmetics and beverages or to assay for enzymes that degrade<br />

ATP and to quantitate ATP in biological fluids.<br />

Features:<br />

• Less Variation: Stable light output.<br />

• User Friendly: Easy-to-prepare reagents.<br />

• Performance: Fast and convenient assay method.<br />

• Sensitive: Detects as little as 10 –15 moles of ATP.<br />

Storage Conditions: Store at –20°C unopened. See product insert for<br />

individual component storage conditions before and after opening.<br />

Protocol Part#<br />

ENLITEN ® ATP Assay System Bioluminescence Detection Kit for ATP<br />

Measurement Technical Bulletin<br />

TB267<br />

ENLITEN ® rLuciferase/Luciferin Reagent<br />

Product Size Cat.# Price ($)<br />

ENLITEN ® rLuciferase/Luciferin Reagent 100 assays FF2021 272.00<br />

Description: The ENLITEN ® rLuciferase/Luciferin Reagent is intended for rapid<br />

and quantitative detection of ATP in liquid samples. The reagent is designed to<br />

measure 10 –11 to 10 –15 moles of ATP. Some applications may include indirect<br />

measurement of bacteria, yeasts and fungi on surfaces or in products, assaying<br />

enzymes that degrade ATP or quantitation of ATP in biological fluids.<br />

Features:<br />

• Less Variation: Stable light output.<br />

• User Friendly: Easy-to-prepare reagents.<br />

• Performance: Fast and convenient assay method.<br />

• Sensitive: Detects as little as 10 –15 moles of ATP.<br />

Storage Conditions: Store at –20°C.<br />

Protocol Part#<br />

ENLITEN ® rLuciferase/rLuciferin Reagent A Bioluminescence<br />

Detection Reagent for ATP Measurement Technical Bulletin<br />

TB268<br />

QuantiLum ® Recombinant Luciferase<br />

Product Size Conc. Cat.# Price ($)<br />

QuantiLum ® Recombinant<br />

1 mg 10–15 mg/ml E1701 147.00<br />

Luciferase<br />

5 mg 10–15 mg/ml E1702 570.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

Description: QuantiLum ® Recombinant Luciferase is expressed from a cloned<br />

gene from the North American firefly (Photinus pyralis) that provides the reliability<br />

and dependability needed for performing research or producing kits using<br />

bioluminescence reagents to detect ATP or luciferin substrates. A<br />

recombinant source eliminates the possibility of seasonal and regional<br />

variability that may be found in luciferase purified from natural sources.<br />

Features:<br />

• Value: Product available in bulk for large orders to suit individual needs<br />

and requirements.<br />

• Reliable: Long-term supply assurance.<br />

• Consistent: Excellent lot-to-lot consistency.<br />

• Choose Your Configuration: Learn more about our custom options for<br />

this product at: www.promega.com/myway/<br />

Storage Conditions: Store at –70°C. Avoid multiple freeze-thaw cycles.<br />

For complete and up-to-date product information visit: www.promega.com/catalog

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