2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 178 ViviRen In Vivo Renilla Luciferase Substrate Product Size Cat.# Price ($) ViviRen In Vivo Renilla Luciferase Substrate 0.37 mg P1231 192.00 For Research Use Only. Not for Use in Diagnostic Procedures. 3.7 mg P1232 Pls. Enq. Description: ViviRen in vivo Renilla Luciferase Substrate is a uniquely engineered coelenterazine-based compound with protected oxidation sites. These modifications are designed to minimize substrate degradation and autoluminescence. It is reported that the ViviRen Substrate demonstrates brighter output when compared to the native coelenterazine substrate when used in an in vivo imaging application in a mouse model. Cat.# P1231 is supplied as a liquid, 60mM in DMSO. Cat.# P1232 is supplied as a lyophilized solid. VivoGlo In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. 9540MA pGL4 in vivo Imaging Vectors Product Size Cat.# Price ($) pGL4.50[luc2/CMV/Hygro] Vector 20 µg E1310 545.00 pGL4.51[luc2/CMV/Neo] Vector For Research Use Only. Not for Use in Diagnostic Procedures. 20 µg E1320 545.00 Description: The pGL4 Luciferase Reporter Vectors are the next generation of reporter gene vectors optimized for expression in mammalian cells. Numerous configurations of pGL4 Vectors are available, including those with the synthetic firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells. Furthermore, both the reporter genes and the vector backbone, including the ampicillin (Amp r ) gene and mammalian selectable marker genes for hygromycin (Hyg r ), neomycin (Neo r ) and puromycin (Puro r ), have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription. The pGL4 Vector backbone is provided with either the luc2 or hRluc gene and, in certain vectors, one or both of two Rapid Response Reporter genes. The proteins encoded by these Rapid Response Luciferase genes respond more quickly and with greater magnitude to changes in transcriptional activity than their more stable counterparts. Vectors are available with a minimal promoter upstream of the lucP reporter gene, useful for cloning in a response element of choice. Vectors also are available with a minimal promoter plus choice of response elements or other promoter elements that can be used to study cellular signaling and other events. These vectors can be used in transient transfections or to generate a stable cell line. All vectors use a destabilized luc2P firefly luciferase gene, resulting in low backgrounds and high levels of induction. Stable cell selection is possible using hygromycin resistance. Features: Improved Sensitivity and Biological Relevance Due to: • Increased Reporter Gene Expression: Codon optimization of synthetic genes for mammalian expression. • Reduced Background and Risk of Expression Artifacts: Removal of cryptic DNA regulatory elements and transcription factor binding sites. • Improved Temporal Response: Rapid Response technology available using destabilized luciferase genes. Additional Advantages Include: • Flexible Detection Options: Choice of either synthetic luc2 (Photinus pyralis) or hRluc (Renilla reniformis) reporter genes. • Easy Transition from Transient to Stable Cells: Choice of mammalian selectable markers. • Easy Transfer from Vector to Vector: Common multiple cloning site and a unique SfiI transfer scheme. Storage Conditions: Store at –20°C. Protocol Part# pGL4.50[luc2/CMV/Hygro] Vector Product Information 9PIE131 pGL4.51[luc2/CMV/Neo] Vector Product Information 9PIE132 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling For complete and up-to-date product information visit: www.promega.com/catalog 9 Industrial and Environmental Monitoring DNA Purification from Food 180 Microbial Detection and Quantitation 180 Protein Deamidation Detection 182 179 9 Industrial and Environmental Monitoring Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
Page 131 and 132: Cell Signaling Maxwell ® 16 System
Page 133 and 134: Cell Signaling RNasin ® Plus RNase
Page 135 and 136: Cell Signaling DNA Quantitation Qua
Page 137 and 138: Cell Signaling Magnetic Stands and
Page 139 and 140: Cell Signaling 6 Drug Discovery For
Page 141 and 142: Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144: Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146: Cell Signaling Protease Assays Prot
Page 147 and 148: Cell Signaling Cell-Based Proteasom
Page 149 and 150: Cell Signaling Tryptase, Human, Rec
Page 151 and 152: Cell Signaling 7 Genetic Identity F
Page 153 and 154: Cell Signaling Maxwell ® 16 Forens
Page 155 and 156: Cell Signaling Plexor ® HY System
Page 157 and 158: Cell Signaling PowerPlex ® 18D Sys
Page 159 and 160: Cell Signaling PowerPlex ® CS7 Sys
Page 161 and 162: Cell Signaling PowerPlex ® 16 and
Page 163 and 164: Cell Signaling GenePrint ® Fluores
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Page 167 and 168: Cell Signaling HaloTag ® Ligand Bu
Page 169 and 170: Cell Signaling Block & Sample 5X Bu
Page 171 and 172: Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174: Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176: Cell Signaling Anti-Human GDNF pAb
Page 177 and 178: Cell Signaling Anti-PARP p85 Fragme
Page 179 and 180: Cell Signaling Anti-VAChT pAb Produ
Page 181: Cell Signaling VivoGlo Caspase 3/7
Page 185 and 186: Cell Signaling Microbial Detection
Page 187 and 188: 10 Instruments For complete and up-
Page 189 and 190: GloMax ® 20/20 Luminometer Product
Page 191 and 192: Fluorescence Module: Application-op
Page 193 and 194: GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196: Maxwell ® 16 Instruments Maxwell
Page 197 and 198: Maxwell ® 16 Flexi Method Firmware
Page 201 and 202: Cell Signaling Maxwell ® 16 System
Page 203 and 204: Cell Signaling Maxwell ® 16 Flexi
Page 205 and 206: Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR For complete
Page 209 and 210: Cell Signaling TaqBead Hot Start Po
Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
Page 219 and 220: Cell Signaling Reverse Transcriptio
Page 221 and 222: Cell Signaling AMV Reverse Transcri
Page 223 and 224: Cell Signaling PCR Cloning pGEM ®
Page 227 and 228: Cell Signaling TnT ® Quick Coupled
Page 229 and 230: Cell Signaling TnT ® T7 Quick for
Page 231 and 232: Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
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Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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