2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
178
ViviRen In Vivo Renilla Luciferase
Substrate
Product Size Cat.# Price ($)
ViviRen In Vivo Renilla Luciferase Substrate 0.37 mg P1231 192.00
For Research Use Only. Not for Use in Diagnostic Procedures.
3.7 mg P1232 Pls. Enq.
Description: ViviRen in vivo Renilla Luciferase Substrate is a uniquely
engineered coelenterazine-based compound with protected oxidation sites.
These modifications are designed to minimize substrate degradation and
autoluminescence. It is reported that the ViviRen Substrate demonstrates
brighter output when compared to the native coelenterazine substrate when
used in an in vivo imaging application in a mouse model.
Cat.# P1231 is supplied as a liquid, 60mM in DMSO. Cat.# P1232 is supplied
as a lyophilized solid.
VivoGlo In Vivo Imaging Substrates are provided in cooperation with Xenogen
Corporation and Caliper Life Sciences for use in in vivo bioluminescence
imaging applications.
Features:
• Highest Quality Substrates: Eliminate potential interference in assays
due to the presence of endotoxins.
• Assured Product Integrity: Most products are packaged in amber vials
with septa to ensure product integrity as well as offer ease of dilution and
use for imaging experiments. Product is packaged with fine tolerances to
minimize the need to weigh substrates.
• Flexibility and Convenience: Available in multiple sizes to accommodate
a variety of experimental settings.
Storage Conditions: Store at –20°C. 9540MA
pGL4 in vivo Imaging Vectors
Product Size Cat.# Price ($)
pGL4.50[luc2/CMV/Hygro] Vector 20 µg E1310 545.00
pGL4.51[luc2/CMV/Neo] Vector
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg E1320 545.00
Description: The pGL4 Luciferase Reporter Vectors are the next generation of
reporter gene vectors optimized for expression in mammalian cells. Numerous
configurations of pGL4 Vectors are available, including those with the synthetic
firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which
have been codon optimized for more efficient expression in mammalian cells.
Furthermore, both the reporter genes and the vector backbone, including the
ampicillin (Amp r ) gene and mammalian selectable marker genes for
hygromycin (Hyg r ), neomycin (Neo r ) and puromycin (Puro r ), have been
engineered to reduce the number of consensus transcription factor binding
sites, reducing background and the risk of anomalous transcription.
The pGL4 Vector backbone is provided with either the luc2 or hRluc gene and,
in certain vectors, one or both of two Rapid Response Reporter genes. The
proteins encoded by these Rapid Response Luciferase genes respond more
quickly and with greater magnitude to changes in transcriptional activity than
their more stable counterparts. Vectors are available with a minimal promoter
upstream of the lucP reporter gene, useful for cloning in a response element of
choice. Vectors also are available with a minimal promoter plus choice of
response elements or other promoter elements that can be used to study
cellular signaling and other events. These vectors can be used in transient
transfections or to generate a stable cell line. All vectors use a destabilized
luc2P firefly luciferase gene, resulting in low backgrounds and high levels of
induction. Stable cell selection is possible using hygromycin resistance.
Features:
Improved Sensitivity and Biological Relevance Due to:
• Increased Reporter Gene Expression: Codon optimization of synthetic
genes for mammalian expression.
• Reduced Background and Risk of Expression Artifacts: Removal of
cryptic DNA regulatory elements and transcription factor binding sites.
• Improved Temporal Response: Rapid Response technology available
using destabilized luciferase genes.
Additional Advantages Include:
• Flexible Detection Options: Choice of either synthetic luc2 (Photinus
pyralis) or hRluc (Renilla reniformis) reporter genes.
• Easy Transition from Transient to Stable Cells: Choice of mammalian
selectable markers.
• Easy Transfer from Vector to Vector: Common multiple cloning site and
a unique SfiI transfer scheme.
Storage Conditions: Store at –20°C.
Protocol Part#
pGL4.50[luc2/CMV/Hygro] Vector Product Information 9PIE131
pGL4.51[luc2/CMV/Neo] Vector Product Information 9PIE132
For complete and up-to-date product information visit: www.promega.com/catalog