2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 176 Horseradish Peroxidase-Conjugated Antibodies Product Size Cat.# Price ($) Anti-Rabbit IgG (H+L), HRP Conjugate 300 µl W4011 148.00 Anti-Mouse IgG (H+L), HRP Conjugate 300 µl W4021 148.00 Anti-Human IgG (H+L), HRP Conjugate 300 µl W4031 148.00 Anti-Chicken IgY, HRP Conjugate 300 µl G1351 198.00 Donkey Anti-Goat IgG, HRP 60 µl V8051 179.00 W4011, W4021, W4031, V8051 For Laboratory Use. G1351 For Research Use Only. Not for Use in Diagnostic Procedures. Description: These high-quality antibodies are raised in goat, rabbit or donkey, affinity-purified and conjugated to horseradish peroxidase. The human, mouse and rabbit antibodies react with all IgG subclasses. All antibodies may crossreact with immunoglobulins of other species. Goat Anti-Rabbit IgG (H+L), HRP Conjugate is an affinity-purified goat anti-rabbit antibody. This antibody has been isolated from antisera by immunoaffinity chromatography using immobilized antigens. This anti-rabbit antibody reacts with heavy and light chains of rabbit IgG. A working dilution of 1:2,500 is suggested for Western blots and ELISAs. Goat Anti-Mouse IgG (H+L), HRP Conjugate is an affinity-purified goat anti-mouse antibody. This antibody has been isolated from antisera by immunoaffinity chromatography using immobilized antigens. This anti-mouse antibody reacts with all subclasses of mouse IgG as well as with light chains on other mouse immunoglobulins. A working dilution of 1:2,500 is suggested for Western blots and ELISAs. Goat Anti-Human IgG (H+L), HRP Conjugate is an affinity-purified goat anti-human antibody. This product has been isolated from antisera by immunoaffinity chromatography using immobilized antigens. This anti-human antibody reacts with all subclasses of human IgG as well as with light chains on other human immunoglobulins. A working dilution of 1:2,500 is suggested for Western blots and ELISAs. Rabbit Anti-Chicken IgY, HRP Conjugate is a secondary antibody developed in rabbits against chicken IgY; it has been affinity-purified and conjugated to horseradish peroxidase. Anti-Chicken IgY, HRP Conjugate recognizes both the heavy and light chains of IgY and has been validated for use in Western blots, dot blots and ELISAs. Donkey Anti-Goat IgG, HRP Conjugate is a secondary antibody developed in donkeys against goat IgG; it has been affinity-purified and conjugated to horseradish peroxidase. When used in a Western blot at a 1:10,000 dilution with colorimetric detection, Donkey Anti-Goat IgG, HRP Conjugate shows reactivity to goat and sheep IgG but minimal cross-reactivity to rabbit and mouse IgG and extracts of PC12 cells. It detects 100ng of goat IgG with minimal background signal. Anti-Chicken IgY, HRP Conjugate Product Size Cat.# Price ($) Anti-Chicken IgY, HRP Conjugate For Research Use Only. Not for Use in Diagnostic Procedures. 300 µl G1351 198.00 Description: This secondary antibody is developed in rabbits against chicken IgY, and has been affinity purified and conjugated to horseradish peroxidase. The Anti-Chicken IgY, HRP Conjugate recognizes both the heavy and light chains of IgY. This antibody has been validated for use in Western blots, dot blots and ELISAs. Formulation: 1mg/ml in 10mM KPO 4 (pH 7.6), 0.15M NaCl, 10mg/ml BSA and 0.01% gentamicin. Storage Conditions: Store at –20°C. Avoid multiple freeze-thaw cycles. When stored and handled properly, this product is stable for 6 months from date of purchase. Chicken IgY, Control Immunoglobulin Product Size Cat.# Price ($) Chicken IgY, Control Immunoglobulin For Laboratory Use. 1 mg G1161 216.00 Description: Chicken IgY, Control Immunoglobulin is specific to chickens and is the counterpart to IgG from mammals. Chicken IgY may be used as a control antigen when performing Westerns, ELISAs and in vivo and in vitro immunohistochemical studies using anti-chicken IgY antibodies. The antibody is prepared by sequential precipitation, is chromatography-purified and is provided at 1mg/ml in PBS with no preservatives. The purity is >75%. TMB One Solution Product Size Cat.# Price ($) TMB One Solution For Laboratory Use. 100 ml G7431 144.00 Description: TMB One Solution is a chromagen substrate, 3,3´,5,5´- tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for horseradish peroxidase detection in an ELISA format. The substrate is provided as a single solution at a ready-to-use working dilution. The substrate develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint multiwell assay after the addition of an acid solution provided by the end user. Features: • Convenient: Single solution provided ready-to-use; just add, incubate, stop and read. This homogeneous reagent improves assay variation. • Stable: Stable for 12 months at 4°C, providing extended shelf life; the assay end product is stable for at least one hour after stopping the assay. • Safe: Provided in a slightly acidic, nonhazardous proprietary buffer without aprotic solvents; noncaustic to plastics used in automated systems. • Sensitive: Low background provides greater assay sensitivity. Storage Conditions: Store at 4°C protected from light. In vivo Imaging VivoGlo Luciferin, In Vivo Grade Product Size Cat.# Price ($) VivoGlo Luciferin, In Vivo Grade 50 mg P1041 269.00 250 mg P1042 569.00 For Research Use Only. Not for Use in Diagnostic Procedures. 1 g P1043 1199.00 Description: Luciferase genes from the North American firefly (Photinus pyralis) and from other beetles are commonly used as light-emitting reporters in cellular and animal models. VivoGlo Luciferin is the potassium salt of d-luciferin, the firefly luciferase substrate capable of generating light when a suitable model is used. VivoGlo In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. 9540MA For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling VivoGlo Caspase 3/7 Substrate (Z-DEVD- Aminoluciferin Sodium Salt) Product Size Cat.# Price ($) VivoGlo Caspase-3/7 Substrate (Z-DEVD- 50 mg P1781 1559.00 Aminoluciferin, Sodium Salt) 5 × 50 mg P1782 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Description: VivoGlo Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) is a firefly luciferase prosubstrate containing the DEVD tetrapeptide sequence recognized by caspase-3 and -7. Upon activation of caspase-3 or -7, the DEVD peptide is cleaved, and the liberated aminoluciferin reacts with luciferase to generate measurable light. Cleavage has been shown with in cellulo and in vivo systems. For mice, activity of a related salt was demonstrated when 10mg of the substrate in 150μl of saline was injected intraperitoneally. Other references suggest that doses as low as 1.5mg per mouse (50mg/kg) can be used. We recommend conducting a preliminary dose-response study using no more than 500mg/kg. VivoGlo Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) has a minimum solubility of 500mg/ml in PBS, and the resulting solution is stable for at least 3 days at room temperature. Injection is usually done via the intraperitoneal route, and imaging is generally started 10 minutes after injection. VivoGlo In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. 9540MA Protocol Part# VivoGlo Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) Quick Protocol FB107 For complete and up-to-date product information visit: www.promega.com/catalog VivoGlo Luciferin-β-Galactosidase Substrate (6-O-β-galactopyranosyl luciferin) Product Size Cat.# Price ($) VivoGlo Luciferin-β-Galactoside Substrate 50 mg P1061 1181.00 (6-O-β-galactopyranosyl luciferin) For Research Use Only. Not for Use in Diagnostic Procedures. 250 mg P1062 3855.00 Description: Luciferin-β-galactoside is a substrate for the commonly used reporter enzyme β-galactosidase. The substrate is cleaved by β-galactosidase to form luciferin and galactose. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light. VivoGlo In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. 9540MA EnduRen In Vivo Renilla Luciferase Substrate Product Size Cat.# Price ($) EnduRen In Vivo Renilla Luciferase Substrate 0.34 mg P1111 231.00 For Research Use Only. Not for Use in Diagnostic Procedures. 3.4 mg P1112 Pls. Enq. Description: EnduRen in vivo Renilla Luciferase Substrate is a uniquely engineered coelenterazine-based compound with protected oxidation sites. These modifications are designed to minimize substrate degradation and autoluminescence. It is reported that EnduRen Substrate may have a longer kinetic output when compared to the native coelenterazine substrate when used in an in vivo imaging application in a mouse model. VivoGlo In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. 9540MA 177 8 Imaging and Immunological Detection Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
Page 129 and 130: Cell Signaling PureYield RNA Midipr
Page 131 and 132: Cell Signaling Maxwell ® 16 System
Page 133 and 134: Cell Signaling RNasin ® Plus RNase
Page 135 and 136: Cell Signaling DNA Quantitation Qua
Page 137 and 138: Cell Signaling Magnetic Stands and
Page 139 and 140: Cell Signaling 6 Drug Discovery For
Page 141 and 142: Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144: Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146: Cell Signaling Protease Assays Prot
Page 147 and 148: Cell Signaling Cell-Based Proteasom
Page 149 and 150: Cell Signaling Tryptase, Human, Rec
Page 151 and 152: Cell Signaling 7 Genetic Identity F
Page 153 and 154: Cell Signaling Maxwell ® 16 Forens
Page 155 and 156: Cell Signaling Plexor ® HY System
Page 157 and 158: Cell Signaling PowerPlex ® 18D Sys
Page 159 and 160: Cell Signaling PowerPlex ® CS7 Sys
Page 161 and 162: Cell Signaling PowerPlex ® 16 and
Page 163 and 164: Cell Signaling GenePrint ® Fluores
Page 165 and 166: Cell Signaling For complete and up-
Page 167 and 168: Cell Signaling HaloTag ® Ligand Bu
Page 169 and 170: Cell Signaling Block & Sample 5X Bu
Page 171 and 172: Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174: Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176: Cell Signaling Anti-Human GDNF pAb
Page 177 and 178: Cell Signaling Anti-PARP p85 Fragme
Page 179: Cell Signaling Anti-VAChT pAb Produ
Page 185 and 186: Cell Signaling Microbial Detection
Page 187 and 188: 10 Instruments For complete and up-
Page 189 and 190: GloMax ® 20/20 Luminometer Product
Page 191 and 192: Fluorescence Module: Application-op
Page 193 and 194: GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196: Maxwell ® 16 Instruments Maxwell
Page 197 and 198: Maxwell ® 16 Flexi Method Firmware
Page 201 and 202: Cell Signaling Maxwell ® 16 System
Page 203 and 204: Cell Signaling Maxwell ® 16 Flexi
Page 205 and 206: Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR For complete
Page 209 and 210: Cell Signaling TaqBead Hot Start Po
Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
Page 219 and 220: Cell Signaling Reverse Transcriptio
Page 221 and 222: Cell Signaling AMV Reverse Transcri
Page 223 and 224: Cell Signaling PCR Cloning pGEM ®
Page 227 and 228: Cell Signaling TnT ® Quick Coupled
Page 229 and 230: Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
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Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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