2012 Promega catalogue

Life
Science
Catalog
2012
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Cell Signaling
176
Horseradish Peroxidase-Conjugated
Antibodies
Product Size Cat.# Price ($)
Anti-Rabbit IgG (H+L), HRP Conjugate 300 µl W4011 148.00
Anti-Mouse IgG (H+L), HRP Conjugate 300 µl W4021 148.00
Anti-Human IgG (H+L), HRP Conjugate 300 µl W4031 148.00
Anti-Chicken IgY, HRP Conjugate 300 µl G1351 198.00
Donkey Anti-Goat IgG, HRP 60 µl V8051 179.00
W4011, W4021, W4031, V8051 For Laboratory Use. G1351 For Research Use Only. Not for Use
in Diagnostic Procedures.
Description: These high-quality antibodies are raised in goat, rabbit or donkey,
affinity-purified and conjugated to horseradish peroxidase. The human, mouse
and rabbit antibodies react with all IgG subclasses. All antibodies may crossreact
with immunoglobulins of other species.
Goat Anti-Rabbit IgG (H+L), HRP Conjugate is an affinity-purified goat
anti-rabbit antibody. This antibody has been isolated from antisera by
immunoaffinity chromatography using immobilized antigens.
This anti-rabbit antibody reacts with heavy and light chains of rabbit IgG. A
working dilution of 1:2,500 is suggested for Western blots and ELISAs.
Goat Anti-Mouse IgG (H+L), HRP Conjugate is an affinity-purified goat
anti-mouse antibody. This antibody has been isolated from antisera by
immunoaffinity chromatography using immobilized antigens.
This anti-mouse antibody reacts with all subclasses of mouse IgG as well
as with light chains on other mouse immunoglobulins. A working dilution of
1:2,500 is suggested for Western blots and ELISAs.
Goat Anti-Human IgG (H+L), HRP Conjugate is an affinity-purified goat
anti-human antibody. This product has been isolated from antisera by
immunoaffinity chromatography using immobilized antigens.
This anti-human antibody reacts with all subclasses of human IgG as well
as with light chains on other human immunoglobulins. A working dilution of
1:2,500 is suggested for Western blots and ELISAs.
Rabbit Anti-Chicken IgY, HRP Conjugate is a secondary antibody
developed in rabbits against chicken IgY; it has been affinity-purified and
conjugated to horseradish peroxidase. Anti-Chicken IgY, HRP Conjugate
recognizes both the heavy and light chains of IgY and has been validated for
use in Western blots, dot blots and ELISAs.
Donkey Anti-Goat IgG, HRP Conjugate is a secondary antibody developed
in donkeys against goat IgG; it has been affinity-purified and conjugated to
horseradish peroxidase. When used in a Western blot at a 1:10,000 dilution
with colorimetric detection, Donkey Anti-Goat IgG, HRP Conjugate shows
reactivity to goat and sheep IgG but minimal cross-reactivity to rabbit and
mouse IgG and extracts of PC12 cells. It detects 100ng of goat IgG with
minimal background signal.
Anti-Chicken IgY, HRP Conjugate
Product Size Cat.# Price ($)
Anti-Chicken IgY, HRP Conjugate
For Research Use Only. Not for Use in Diagnostic Procedures.
300 µl G1351 198.00
Description: This secondary antibody is developed in rabbits against chicken
IgY, and has been affinity purified and conjugated to horseradish peroxidase.
The Anti-Chicken IgY, HRP Conjugate recognizes both the heavy and light
chains of IgY. This antibody has been validated for use in Western blots, dot
blots and ELISAs.
Formulation: 1mg/ml in 10mM KPO 4 (pH 7.6), 0.15M NaCl, 10mg/ml BSA
and 0.01% gentamicin.
Storage Conditions: Store at –20°C. Avoid multiple freeze-thaw cycles. When
stored and handled properly, this product is stable for 6 months from date of
purchase.
Chicken IgY, Control Immunoglobulin
Product Size Cat.# Price ($)
Chicken IgY, Control Immunoglobulin
For Laboratory Use.
1 mg G1161 216.00
Description: Chicken IgY, Control Immunoglobulin is specific to chickens and
is the counterpart to IgG from mammals. Chicken IgY may be used as a control
antigen when performing Westerns, ELISAs and in vivo and in vitro
immunohistochemical studies using anti-chicken IgY antibodies. The antibody
is prepared by sequential precipitation, is chromatography-purified and is
provided at 1mg/ml in PBS with no preservatives. The purity is >75%.
TMB One Solution
Product Size Cat.# Price ($)
TMB One Solution
For Laboratory Use.
100 ml G7431 144.00
Description: TMB One Solution is a chromagen substrate, 3,3´,5,5´-
tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for
horseradish peroxidase detection in an ELISA format. The substrate is provided
as a single solution at a ready-to-use working dilution. The substrate develops
a blue reaction product when oxidized by peroxidase and a yellow reaction
product in an endpoint multiwell assay after the addition of an acid solution
provided by the end user.
Features:
• Convenient: Single solution provided ready-to-use; just add, incubate,
stop and read. This homogeneous reagent improves assay variation.
• Stable: Stable for 12 months at 4°C, providing extended shelf life; the
assay end product is stable for at least one hour after stopping the assay.
• Safe: Provided in a slightly acidic, nonhazardous proprietary buffer without
aprotic solvents; noncaustic to plastics used in automated systems.
• Sensitive: Low background provides greater assay sensitivity.
Storage Conditions: Store at 4°C protected from light.
In vivo Imaging
VivoGlo Luciferin, In Vivo Grade
Product Size Cat.# Price ($)
VivoGlo Luciferin, In Vivo Grade 50 mg P1041 269.00
250 mg P1042 569.00
For Research Use Only. Not for Use in Diagnostic Procedures.
1 g P1043 1199.00
Description: Luciferase genes from the North American firefly (Photinus
pyralis) and from other beetles are commonly used as light-emitting reporters
in cellular and animal models. VivoGlo Luciferin is the potassium salt of
d-luciferin, the firefly luciferase substrate capable of generating light when a
suitable model is used.
VivoGlo In Vivo Imaging Substrates are provided in cooperation with Xenogen
Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging
applications.
Features:
• Highest Quality Substrates: Eliminate potential interference in assays
due to the presence of endotoxins.
• Assured Product Integrity: Most products are packaged in amber vials
with septa to ensure product integrity as well as offer ease of dilution and
use for imaging experiments. Product is packaged with fine tolerances to
minimize the need to weigh substrates.
• Flexibility and Convenience: Available in multiple sizes to accommodate
a variety of experimental settings.
Storage Conditions: Store at –20°C. 9540MA
For complete and up-to-date product information visit: www.promega.com/catalog