2012 Promega catalogue

Cell Signaling
Anti-VAChT pAb
Product Size Cat.# Price ($)
Anti-VAChT pAb 100 µg G4481 296.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The purified Anti-VAChT (Vesicular Acetylcholine Transporter) pAb
is raised in goats against a peptide (CSPPGPFDGCEDDYNYYSRS) corresponding
to amino acids 511–530 of the carboxy terminus of the cloned rat VAChT. It is a
novel tool to identify functional cholinergic neurons in the central and peripheral
nervous system where the antibody stains fibers and neuronal cell bodies. This
antibody has application for the study of the pathophysiology of neurodegenerative
diseases involving the cholinergic system and for mapping cholinergic
neurons in the nervous system.
Features:
• Immunogen: Carboxy-terminal peptide sequence 511–530 corresponding
to cloned rat VAChT protein.
• Antibody Form: Purified goat polyclonal IgG; 0.5mg/ml in PBS containing
no preservatives.
• Specificity: Cross-reacts with VAChT in rat and mouse, but not in human,
guinea pig, rabbit or cat.
Storage Conditions: Store at –20°C.
Immunofluorescence staining for VAChT protein in ventral gray matter
of rat spinal cord. VAChT was detected using Anti-VAChT pAb at
1μg/ml followed by anti-goat Cy ® 3 conjugate. Specificity was demonstrated by
preabsorption of Anti-VAChT pAb (1μg/ml) with the peptide immunogen
(5μg/ml) prior to incubation with the primary antibody and anti-goat Cy ® 3
conjugate (data not shown). Protocols developed and performed at Promega.
Donkey Anti-Rabbit IgG (H+L) HRP, Anti-
ACTIVE ® Qualified
Product Size Cat.# Price ($)
Donkey Anti-Rabbit IgG (H+L), HRP
For Laboratory Use.
60 µl V7951 179.00
Description: Donkey Anti-Rabbit Ig, (H+L), HRP, are horseradish
peroxidase-conjugated secondary antibodies specifically tested for use with
the Anti-ACTIVE ® antibodies. They are qualified for use in Western blot analysis
using chemiluminescent and colorimetric detection methods. These antibody
conjugates exhibit minimal cross-reactivity to goat, mouse and sheep IgG,
bovine serum albumin (BSA) and proteins in mammalian cell extracts. These
secondary antibody conjugates provide low background and highly specific
signals when used at the recommended dilutions with Anti-ACTIVE ® MAPK,
Anti-ACTIVE ® JNK and Anti-ACTIVE ® p38 pAbs. The conjugates are provided
in phosphate-buffered saline containing BSA as a stabilizer and gentamicin as
a preservative.
For complete and up-to-date product information visit: www.promega.com/catalog
2018TB
Features:
• Sensitivity: When conjugates are used in a Western blot at a 1:10,000
dilution along with Anti-ACTIVE ® MAPK pAb (Cat.# V8031), they can detect
active MAP kinase in 5μg of activated (nerve growth factor [NGF]-treated)
PC12 cell extract using colorimetric detection.
• Specificity: Preferentially detects rabbit IgG with minimum reactivity with
immunoglobulins from other species (including goat, sheep and mouse) or
with bovine serum albumin and mammalian cell extract proteins.
• Value: 60μl per vial, sufficient to generate 300–600ml of Western blotting
solution when used at the recommended dilution of 1:5,000 to 1:10,000.
• Immunogen: Intact rabbit IgG (H+L chains).
• Antibody Form: Donkey IgG, affinity-purified polyclonal antibody
conjugated to horseradish peroxidase (HRP).
Storage Conditions: Store at –20°C.
Protocol Part#
TB262
Anti-ACTIVE ® MAPK, JNK and p38 Polyclonal Antibodies and
Anti-ACTIVE ® Qualified Secondary Antibody Conjugates
Technical Bulletin
Anti-ACTIVE ® CaM KII pAb, (pT286 ) and Anti-ACTIVE ® Qualified
Secondary Antibody Conjugates Technical Bulletin
TB264
Alkaline Phosphatase-Conjugated Antibodies
Product Size Cat.# Price ($)
Anti-Mouse IgG (H+L), AP Conjugate 100 µl S3721 148.00
Anti-Rabbit IgG (Fc), AP Conjugate 100 µl S3731 148.00
Anti-Human IgG (H+L), AP Conjugate 100 µl S3821 148.00
Anti-Rat IgG (H+L), AP Conjugate 100 µl S3831 148.00
Donkey Anti-Goat IgG, AP
For Laboratory Use.
60 µl V1151 179.00
Description: The Alkaline Phosphatase-Conjugated Antibodies are used in the
detection of proteins in Western blotting and ELISAs. The alkaline phosphatase
(AP) catalyzes colorimetric reactions using Western Blue ® Substrate (standard
BCIP/NBT) or AttoPhos ® Reagent. It can also drive the chemiluminescent
detection reactions involving substrates such as 3-(2´-spiroadamantane)-
4-methyl-4-(3´´ phosphoryloxyphenyl-1, 2-dioxetane (AMPPD ® ).
Goat Anti-Mouse IgG (H+L), Alkaline Phosphatase Conjugate is an
affinity-purified goat anti-mouse antibody. It reacts with mouse IgG (all
subclasses) as well as with light chains on other mouse immunoglobulins.
Antibodies may cross-react with immunoglobulins from other species.
Goat Anti-Rabbit IgG (Fc), Alkaline Phosphatase Conjugate is an affinitypurified
goat anti-rabbit antibody. It reacts with the heavy chains of rabbit IgG
but not with light chains. Antibodies may cross-react with immunoglobulins
from other species.
Goat Anti-Human IgG (H+L), Alkaline Phosphatase Conjugate is an
affinity-purified goat anti-human antibody. This product has been isolated from
antisera by immunoaffinity chromatography using immobilized antigens. It
reacts with all subclasses of human IgG as well as with light chains on other
human immunoglobulins. Antibodies may cross-react with immunoglobulins
from other species. This product displays minimal cross-reactivity with horse
and bovine serum proteins.
Goat Anti-Rat IgG (H+L), Alkaline Phosphatase Conjugate is an affinitypurified
goat anti-rat antibody. This product has been isolated from antisera
by immunoaffinity chromatography using immobilized antigens. It reacts with
heavy and light chains of rat IgG. Antibodies may cross-react with
immunoglobulins from other species.
Donkey Anti-Goat IgG, Alkaline Phosphatase Conjugate is a secondary
antibody developed in donkeys against goat IgG; it has been affinity-purified
and conjugated to alkaline phosphatase.
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8
Imaging and Immunological Detection
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