2012 Promega catalogue

Life
Science
Catalog
2012
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Cell Signaling
174
Anti-TGFβ 1 pAb
Product Size Cat.# Price ($)
Anti-TGFβ 1 pAb 100 µg G1221 630.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Transforming growth factor β 1 (TGFβ 1) is a 25kDa homodimer
composed of two 12.5kDa subunits held together by disulfide bonds. TGFβ 1 is
a protein of immense interest to a number of fields and has been associated
with intracellular matrix deposition and tissue repair/damage, cell cycle control
and apoptosis. The Anti-TGFβ 1 pAb is directed against biologically active
human TGFβ 1, providing a useful tool to analyze TGFβ 1 in Western blot analysis
or immunostaining applications.
Features:
• Immunogen: Biologically active human TGFβ 1.
• Antibody Form: Rabbit IgG provided at 1mg/ml in PBS containing
0.02mg/ml gentamicin as a preservative.
• Specificity: Reacts with biologically active TGFβ 1 with no cross-reactivity
to TGFβ 2 and TGFβ 3.
Storage Conditions: Store at –20°C.
Anti-TrkB In pAb
Product Size Cat.# Price ($)
Anti-TrkB In pAb
For Research Use Only. Not for Use in Diagnostic Procedures.
100 µg G1561 368.00
Description: TrkB is the high-affinity receptor for brain-derived neurotrophic
factor (BDNF) and neurotrophin-4 (NT-4). Two forms of the TrkB receptor exist:
the full-length, active 145kDa protein and a truncated, nonsignaling 95kDa
protein. The truncated isoform lacks the cytoplasmic tyrosine kinase catalytic
region and therefore does not signal in response to BDNF or NT-4. The
Anti-TrkB In pAb is a polyclonal antibody raised in chickens against a
cytoplasmic domain of the carboxy terminus of mouse TrkB.
Features:
• Immunogen: A fusion protein containing residues 482–501
(HISNGSNTPSSSEGGPDAVI) derived from a portion of the intracellular
domain of mouse TrkB generated in E. coli using a proprietary synthetic
carrier protein technology.
• Antibody Form: Chicken IgY provided at 0.5mg/ml in 0.1M NaCl, 0.01M
K 2PO 4 (pH 7.4).
• Specificity: Detects full-length TrkB, the high-affinity receptor for BDNF
and NT-4, in human, rat and primate brain.
Storage Conditions: Store at –20°C.
Immunohistochemical staining of rat cerebellum Purkinje cells with
chicken Anti-TrkB In pAb. Visualized with a donkey anti-chicken IgY, biotin
conjugate, followed by VECTASTAIN ® Elite AB reagent, DAB reaction and OsO 4
enhancement. Protocols developed and performed at Promega.
1865TA08_7A
Anti-βIII Tubulin mAb
Product Size Cat.# Price ($)
Anti-βIII Tubulin mAb
For Research Use Only. Not for Use in Diagnostic Procedures.
100 µg G7121 474.00
Description: Anti-βIII Tubulin mAb is a protein G-purified IgG 1 monoclonal
antibody (from clone 5G8) raised in mice against a peptide (EAQGPK)
corresponding to the C-terminus of βIII tubulin. It is directed against βIII tubulin,
a specific marker for neurons. The major use of this antibody is for labeling
neurons in tissue sections and cell culture. The antibody has been tested to
perform in frozen and paraffin-embedded sections of rat brain, cerebellum and
spinal cord, human and rat fetal CNS progenitor cell cultures and adult human
paraffin-embedded brain.
Features:
• Immunogen: Peptide corresponding to the C-terminus (EAQGPK) of βIII
tubulin.
• Antibody Form: Mouse monoclonal IgG 1 (clone 5G8), 1mg/ml in PBS
containing no preservatives.
• Specificity: Cross-reacts with most mammalian species. Does not label
nonneuronal cells (e.g., astrocytes).
Storage Conditions: Store at 4°C.
Immunostaining for βIII tubulin in rat cerebellum using
Anti-βIII Tubulin mAb. Paraffin-embedded rat brain section doubleimmunofluorescence
labeled with the primary antibody and detected using an
anti-mouse Cy ® 3-conjugated secondary antibody (yellow). Nuclei were stained
with DAPI (blue). Protocols developed and performed at Promega.
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2137TA