2012 Promega catalogue

Cell Signaling
Anti-PARP p85 Fragment pAb
Product Size Cat.# Price ($)
Anti-PARP p85 Fragment pAb 50 µl G7341 539.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved
in DNA repair, is a well known substrate for caspase-3 cleavage during
apoptosis. Anti-PARP p85 Fragment pAb is a rabbit polyclonal antibody
specific for the p85 fragment of PARP that results from caspase cleavage of
the 116kDa intact molecule and thus provides an in situ marker for apoptosis.
The antibody is affinity-purified using a peptide that corresponds to a region
of the p85 fragment of PARP. The PARP immunogen is a synthetic peptide,
gly-val-asp-glu-val-ala-lys (GVDEVAK), representing the N-terminus of the large
C-terminal fragment of human PARP that results from caspase-3 cleavage.
Each batch of antibody is quality assurance tested for use in immunostaining
applications and contains sufficient antibody for 50 immunocytochemical
reactions at the suggested working dilution of 1:100.
Features:
• Immunogen: N-terminal peptide from p85 fragment.
• Antibody Form: Affinity-purified rabbit polyclonal antibody provided in
Dulbecco’s PBS.
• Specificity: Specifically detects PARP p85 fragment in human, rat and
bovine cells and tissues. Does not recognize the 116kDa intact PARP
protein.
Storage Conditions: Store at –20°C.
Protocol Part#
Anti-PARP p85 Fragment pAb Technical Bulletin TB273
Anti-PARP p85 Fragment pAb and TUNEL double-labeling of apoptotic
Jurkat cells. Cells were labeled with the Anti-PARP p85 Fragment pAb
(red) and the DeadEnd Fluorometric TUNEL System (Cat.# G3250; green).
The colocalization of cleaved PARP in cells containing TUNEL-positive nuclei
demonstrates that the Anti-PARP p85 Fragment pAb specifically labels
apoptotic cells. Protocols developed and performed at Promega.
For complete and up-to-date product information visit: www.promega.com/catalog
2734TA08_9A
Anti-Human p75 pAb
Product Size Cat.# Price ($)
Anti-Human p75 pAb
For Research Use Only. Not for Use in Diagnostic Procedures.
200 µg G3231 579.00
Description: The p75 neurotrophin receptor (p75 NTR ), also known as lowaffinity
NGF receptor (LNGFR) and p75 LNGFR , binds nerve growth factor, brainderived
neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying
specificities. p75 NTR plays an important role in neurotrophic factor signaling
including neuronal apoptosis. Anti-Human p75 pAb provides a valuable tool for
understanding the role of p75 NTR in neuronal death.
Features:
• Immunogen: Cytoplasmic domain of the human p75 neurotrophin
receptor.
• Antibody Form: Purified rabbit IgG; 1mg/ml in PBS containing 50μg/ml
gentamicin.
• Specificity: Human, rat, mouse and chicken p75.
Storage Conditions: Store at 4°C.
Electron micrograph demonstrating immunostaining with Anti-
Human p75 pAb in the inner molecular layer of the rat dentate gyrus.
An axon terminal containing p75 immunoreactivity (A➤) is seen forming a
synapse with a large unlabeled dendrite. Also labeled are a lengthwise axonal
profile (B➤) and a small axonal cross section (C➤). Pre-embedding (Epon)
immunohistochemistry was visualized with VECTASTAIN ® ABC Reagent.
Myelin sheath appears black due to OsO 4 fixation. Image kindly provided by
Drs. Karen Dougherty and Teresa Milner, Cornell University Medical College.
2029TA01_8A
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Imaging and Immunological Detection
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