2012 Promega catalogue

Cell Signaling
Anti-Human GDNF pAb
Product Size Cat.# Price ($)
Anti-Human GDNF pAb 200 µg G2791 740.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Human glial cell-line-derived neurotrophic factor (GDNF), a
30kDa homodimer, has been shown to be a potent survival factor for a variety
of neurons. The receptor complex for GDNF has been elucidated, though
members of the multicomponent receptor family continue to grow. With
applications in Western blotting, ELISA and immunostaining, the Anti-Human
GDNF pAb is a useful tool to continue the investigation of GDNF’s role in
multiple facets of neurological systems.
Features:
• Immunogen: Human recombinant GDNF.
• Antibody Form: Chicken IgY; 0.5mg/ml in 0.1M NaCl, 0.01M K 2HPO 4,
50μg/ml gentamicin.
• Specificity: Cross-reactive between mammalian species; does not
cross-react with TGFα, TGFβ 1, NGF or BDNF at up to 10μg/ml.
Storage Conditions: Store at 4°C.
Anti-GFAP pAb
Product Size Cat.# Price ($)
Anti-GFAP pAb
For Research Use Only. Not for Use in Diagnostic Procedures.
100 µg G5601 264.00
Description: Anti-GFAP pAb is a polyclonal antibody against glial fibrillary
acidic protein (GFAP), a specific marker of astrocytes in the central nervous
system, and is qualified for immunostaining applications.
Features:
• Immunogen: Purified glial fibrillary acidic protein from bovine spinal cord.
• Antibody Form: Purified rabbit IgG; supplied at 1mg/ml in PBS containing
50μg/ml gentamicin.
• Specificity: Human, bovine and rat GFAP; not recommended for mouse.
Storage Conditions: Store at 4°C.
Anti-GFAP-labeled astrocytes in mixed rat neural progenitor cultures.
Cells were labeled with DAPI (blue) and Anti-GFAP pAb with a
Cy ® 3-conjugated secondary antibody (red). Protocols developed and
performed at Promega.
For complete and up-to-date product information visit: www.promega.com/catalog
2773TA10_9A
Anti-Luciferase pAb
Product Size Cat.# Price ($)
Anti-Luciferase pAb
For Research Use Only. Not for Use in Diagnostic Procedures.
200 µg G7451 291.00
Description: Anti-Luciferase pAb is a goat polyclonal antibody designed for
use in immunocytochemistry and Western blot applications. Anti-Luciferase
pAb can detect luciferase enzyme expression in situ.
Features:
• Immunogen: Recombinant luciferase from North American firefly
(Photinus pyralis).
• Antibody Form: Goat polyclonal IgG at 1mg/ml in PBS containing
50μg/ml gentamicin.
• Specificity: Anti-Luciferase pAb is specific for firefly luciferase (Photinus
pyralis) and does not cross-react with sea pansy (Renilla reniformis)
luciferase.
Storage Conditions: Store at 4°C.
NIH/3T3 cells transiently transfected with a luciferase gene.
Luciferase-expressing cells were detected using the Anti-Luciferase pAb
(Cat.# G7451). Protocols developed and performed at Promega.
2760TA10_9A
171
8
Imaging and Immunological Detection
Section
Contents
Table of
Contents