2012 Promega catalogue

Life
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2012
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Cell Signaling
170
Anti-ACTIVE ® Caspase-3 pAb
Product Size Cat.# Price ($)
Anti-ACTIVE ® Caspase-3 pAb 50 µl G7481 500.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Anti-ACTIVE ® Caspase-3 pAb is intended for use as a marker
of apoptosis; it specifically stains apoptotic cells without staining nonapoptotic
cells. Includes sufficient antibody to perform 125 immunocytochemical assays
(100μl/assay) at a 1:250 dilution.
Features:
• Immunogen: Peptide derived from the p17 fragment of caspase-3 and
having sequence homology in human, mouse, rat and hamster.
• Antibody Form: Affinity-purified rabbit IgG; supplied in Dulbecco’s PBS.
• Specificity: Specifically recognizes the cleaved active form of caspase-3
in human, rat and mouse.
Storage Conditions: Store at –20°C.
Protocol Part#
Anti-ACTIVE ® Caspase-3 pAb Product Information
9PIG748
Demonstration of Anti-ACTIVE ® Caspase-3 pAb positive cells in
postnatal day 0 (P0) mouse brain paraffin-embedded sections.
Panel A. Three Anti-ACTIVE ® Caspase-3 pAb-positive cells (colored arrows).
Panel B. Corresponding DAPI-stained nuclei. Note the correspondence of
Anti-ACTIVE ® Caspase-3 pAb label with the typical apoptotic, condensed
nuclear morphology in Panel B. Protocols developed and performed at
Promega.
3158TB
Anti-β-Galactosidase mAb
Product Size Conc. Cat.# Price ($)
Anti-β-Galactosidase, Purified 100 µg 2.0–2.5 mg/ml Z3781 64.00
Monoclonal Antibody
For Laboratory Use.
2 mg 2.0–2.5 mg/ml Z3783 529.00
Description: This antibody [subclass IgG 2a(κ)] was purified from ascites of a
mouse hybridoma and recognizes E. coli β-galactosidase.
Features:
• Immunogen: β-galactosidase.
• Antibody Form: 2.0–2.5mg/ml in 10mM Tris-HCl (pH 8.0), 150mM NaCl,
0.02% sodium azide.
• Specificity: E. coli β-galactosidase near the C-terminal end.
Storage Conditions: Store undiluted at –20°C.
A. B.
C. D.
E. F.
Histological analysis of axonal termination in the accessory olfactory
bulb (AOB). Sagittal sections through the AOB stained with the
Anti-β-Galactosidase mAb (Cat.# Z3781; green) and antibodies against
synaptophysin (DAKO, red). DAPI staining is shown in blue. Panel A.
Heterozygous VL mouse. Panels B and C. Homozygous VL mouse.
Panel D. Homozygous ∆V mouse. Panel E. Heterozygous MV mouse.
Panel F. Homozygous VG mouse. Details on gene targeting, mutations and
immunostaining may be found in Rodriguez, J., Feinstein, P. and Mombaerts,
P. (1999) Cell 97, 199. Images kindly provided by Dr. Peter Mombaerts, The
Rockefeller University, New York. Reprinted by permission of Cell Press.
For complete and up-to-date product information visit: www.promega.com/catalog
2761TA10_9A