2012 Promega catalogue

Cell Signaling
Anti-ERK 1/2 pAb, Rabbit
Product Size Cat.# Price ($)
Anti-ERK 1/2 pAb, Rabbit 40 µl V1141 367.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Anti-ERK 1/2 pAb is a polyclonal antibody purified from rabbit
serum. The antibody is affinity-purified using a peptide sequence in human/
rat ERK1.
Features:
• Specificity: Detects ERK1 and ERK2 in the nonphosphorylated,
monophosphorylated and dually phosphorylated forms.
• Immunogen: Sequence representing a conserved region in human and rat
ERK1 located outside of the catalytic core of the enzyme.
• Antibody Form: Affinity-purified rabbit IgG; supplied in PBS (pH 7.4).
• Value: When used at the recommended 1:5,000 dilution, this product will
generate up to 200ml of blotting solution, sufficient for 20 Western blots of
10ml each.
Storage Conditions: Store at –20°C.
Protocol Part#
Anti-ERK 1/2 pAb, Rabbit Product Information 9PIV114
kDa
98 –
64 –
50 –
36 –
Anti-ACTIVE ®
MAPK pAb
Anti-ERK
1/2 pAb
1 2 1 2
Detection of the specifically phosphorylated form of MAPK in NGFtreated
PC12 cell extracts. Anti-ACTIVE ® MAPK pAb (Cat.# V8031) and
Anti-ERK 1/2 (“pan ERK 1/2”) pAb (Cat.# V1141) detection of ERK 1/2 in
untreated (lanes 1) or NGF-treated (lanes 2) PC12 cell extracts (2μg).
For complete and up-to-date product information visit: www.promega.com/catalog
2582TB03_9A
Anti-Human BDNF pAb
Product Size Cat.# Price ($)
Anti-Human BDNF pAb
For Research Use Only. Not for Use in Diagnostic Procedures.
200 µg G1641 740.00
Description: BDNF, a 27kDa homodimer originally derived from human brain,
shares high sequence homology with NGF, NT-3 and NT-4/5 and influences
many neuron types in the CNS. Anti-Human BDNF pAb is generated in chickens
and purified using a proprietary polyethylene glycol procedure. IgY, the 180kDa
chicken IgG homolog, can be produced in chickens against certain biological
antigens that fail to elicit a humoral immune response in rabbits or other
mammals due to species relatedness. This antibody is highly specific for BDNF.
Features:
• Immunogen: Human recombinant BDNF.
• Antibody Form: Chicken IgY, provided at 0.5mg/ml in 0.1M NaCl,
0.01M K 2HPO 4 and 50μg/ml gentamicin.
• Specificity: Cross-reactive between mammalian species; does not
cross-react with other neurotrophic factors.
Storage Conditions: Store at 4°C.
Localization of BDNF in primary cultures of hippocampal neurons. The
Anti-Human BDNF pAb was used at a 1:200 dilution. Primary antibody was
detected using HRP-conjugated goat anti-chicken IgY secondary antibody.
Photomicrograph kindly provided by Dr. Laurie Goodman, Lynx Therapeutics,
Hayward, CA. Reprinted by permission of Academic Press, Goodman, L. et al.
(1996) Mol. Cell Neurosci. 7, 222.
1830TA07_7A
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8
Imaging and Immunological Detection
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