2012 Promega catalogue

Life
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2012
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Cell Signaling
168
Anti-ACTIVE ® p38 pAb, Rabbit, (pTGpY)
Product Size Cat.# Price ($)
Anti-ACTIVE ® p38 pAb, Rabbit, (pTGpY) 100 µl V1211 671.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Anti-ACTIVE ® p38 pAb is a polyclonal rabbit antibody. The
antibody is affinity-purified using a dually phosphorylated peptide that
corresponds to the active form of the p38 enzymes.
Features:
• Specificity: Preferentially detects the dually phosphorylated, active form of
p38 kinase.
• Immunogen: Dually phosphorylated Thr/Gly/Tyr region (pTGpY) derived
from the catalytic core of the active form of p38 kinase, which corresponds
to Thr 180 and Tyr 182 of the mammalian p38 enzyme.
• Antibody Form: Affinity-purified rabbit IgG; supplied in PBS (pH 7.4).
• Value: When used at the recommended 1:2,000 dilution, this product will
generate up to 200ml of blotting solution, sufficient for 20 Western blots of
10ml each.
Storage Conditions: Store at –20°C.
Protocol Part#
TB262
Anti-ACTIVE ® MAPK, JNK and p38 Polyclonal Antibodies and
Anti-ACTIVE ® Qualified Secondary Antibody Conjugates
Technical Bulletin
A.
B.
Anti-ACTIVE
NGF
kDa – +
® MAPK
98 –
64 –
50 –
36 –
– ERK1
– ERK2
Anti-ACTIVE
Sorbitol
– +
® JNK
– JNK2
– JNK1
Anti-ACTIVE
Sorbitol
– +
® p38
1 2 3 4 5 6
Anti-MAPK
NGF
kDa – +
98 –
64 –
50 –
36 –
– ERK1
– ERK2
Anti-JNK
Sorbitol
– +
– JNK2
– JNK1
Anti-p38
Sorbitol
– +
1 2 3 4 5 6
– p38
– p38
Detection of MAPK, JNK and p38 in PC12 cell extracts. Panel A.
Western blot analysis using Anti-ACTIVE ® MAPK, Anti-ACTIVE ® JNK and
Anti-ACTIVE ® p38 polyclonal antibodies to detect activated MAPK, JNK and
p38. Panel B. Western blot analysis using anti-MAPK, anti-JNK and anti-p38
antibodies to detect activated and nonactivated MAPK, JNK and p38 in
untreated or NGF- or sorbitol-treated PC12 cells.
1859TC
A. Anti-ACTIVE ® MAPK pAb
NGF-Treated Untreated
B. Anti-ACTIVE ® JNK pAb
Sorbitol-Treated Untreated
C. Anti-ACTIVE ® p38 pAb
Sorbitol-Treated Untreated
Detection of activated MAPK, JNK and p38 in PC12 cells by
immunocytochemistry. PC12 cells were grown to 80% confluence in
RPMI 1640 medium supplemented with 25mM HEPES, 300mg/L l-glutamine,
10% horse serum, 5% fetal bovine serum and 0.5mM EGTA. Cells were either
untreated or treated with 200ng/ml NGF or 1M sorbitol as indicated. ICC was
performed as described in Promega Technical Bulletin #TB262. Anti-ACTIVE ®
antibodies were used at the following dilutions: Panel A. MAPK, 1:500;
Panel B. JNK, 1:1,000; Panel C. p38, 1:500. Protocols developed and
performed at Promega.
For complete and up-to-date product information visit: www.promega.com/catalog
2870TA02_0A