2012 Promega catalogue

Cell Signaling
Anti-ACTIVE ® JNK pAb, Rabbit, (pTPpY)
Product Size Cat.# Price ($)
Anti-ACTIVE ® JNK pAb, Rabbit, (pTPpY) 40 µl V7931 671.00
For Research Use Only. Not for Use in Diagnostic Procedures.
120 µl V7932 1115.00
Description: Anti-ACTIVE ® JNK pAb is a polyclonal antibody from rabbit
serum. The antibody is affinity purified using a dually phosphorylated peptide
that corresponds to the active form of the JNK enzymes.
Features:
• Specificity: Preferentially detects the dually phosphorylated, active form of
the stress-activated protein kinase (SAPK), also known as c-Jun N-terminal
kinase, JNK.
• Immunogen: Dually phosphorylated Thr/Pro/Tyr region (pTPpY) derived
from the catalytic core of the active form of JNK kinase, which corresponds
to Thr 183 and Tyr 185 of the mammalian JNK2 enzyme.
• Antibody Form: Affinity-purified rabbit IgG; supplied in 10mM sodium
phosphate (pH 7.4), 20mM NaCl.
• Value: Anti-ACTIVE ® JNK pAb is available in two convenient sizes.
Cat.# V7931 will generate up to 200ml of blotting solution, sufficient for
20 Western blots of 10ml each. The larger size, Cat.# V7932, will generate
up to 600ml of blotting solution, sufficient for 60 Western blots of 10ml
each.
Storage Conditions: Store at –20°C.
Protocol Part#
TB262
Anti-ACTIVE ® MAPK, JNK and p38 Polyclonal Antibodies and
Anti-ACTIVE ® Qualified Secondary Antibody Conjugates
Technical Bulletin
Immunocytochemical detection of active JNK enzyme in Drosophila
pupal retina using the Anti-ACTIVE ® JNK pAb. Drosophila pupal retina
at 25% of pupal development were fixed in 3% paraformaldehyde in PBS.
The Anti-ACTIVE ® JNK pAb was diluted 1:100 in PBS containing 10%
fetal bovine serum and 0.2% Triton ® X-100. Samples were incubated with
the primary antibody overnight at 4˚C, washed 3 times (10 minutes each)
with 0.2% Triton ® X-100 and then incubated with a goat anti-rabbit Cy ® 3
conjugate for 2 hours at 4˚C. Whole mounts were visualized with a Zeiss ®
Axioskop fluorescent microscope. The results illustrate the presence of dually
phosphorylated, active forms of JNK in discrete structures of the fly retinal
ommatidia including intense staining of the inner cone cells as well as the
mechanosensory bristles and surrounding pigment cells. The pattern of
staining (which was distinct from results obtained with an antibody for active
p38) and the absence of staining in control experiments (data not shown)
support the high specificity of the Anti-ACTIVE ® JNK pAb. Image kindly
provided by David T. Miller and Ross Cagan, Department of Molecular Biology
and Pharmacology, Washington University School of Medicine, St. Louis,
Missouri.
For complete and up-to-date product information visit: www.promega.com/catalog
1825TA07_7A
Anti-ACTIVE ® MAPK pAb, Rabbit, (pTEpY)
Product Size Cat.# Price ($)
Anti-ACTIVE ® MAPK pAb, Rabbit, (pTEpY)
For Research Use Only. Not for Use in Diagnostic Procedures.
40 µl V8031 671.00
Description: Anti-ACTIVE ® MAPK pAb is a polyclonal rabbit antibody. The
antibody is affinity purified using a dually phosphorylated peptide that
corresponds to the active form of the mitogen-activated protein (MAP) kinase
enzymes.
Features:
• Specificity: Preferentially detects the dually phosphorylated, active form of
the mitogen-activated protein kinase (MAPK) enzymes (ERK1 and ERK2).
• Immunogen: Dually phosphorylated Thr/Glu/Tyr region (pTEpY) derived
from the catalytic core of the active form of the mitogen-activated protein
kinase (MAPK) enzymes, ERK1 and ERK2, which corresponds to Thr 183 and
Tyr 185 of the mammalian ERK2 enzyme.
• Antibody Form: Affinity-purified rabbit IgG; supplied in PBS (pH 7.4).
• Value: When used at the recommended 1:5,000 dilution, this product will
generate 200ml of blotting solution, sufficient for 20 Western blots of 10ml
each.
Storage Conditions: Store at –20°C.
Protocol Part#
TB262
Anti-ACTIVE ® MAPK, JNK and p38 Polyclonal Antibodies and
Anti-ACTIVE ® Qualified Secondary Antibody Conjugates
Technical Bulletin
167
8
Imaging and Immunological Detection
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