2012 Promega catalogue

Cell Signaling
Block & Sample 5X Buffer
Product Size Cat.# Price ($)
Block & Sample 5X Buffer 54 ml G3311 88.00
For Laboratory Use.
Description: The Block & Sample 5X Buffer is optimized for use in the E max ®
ImmunoAssay Systems (for BDNF, GDNF and NGF) providing additional buffer
for further sample dilutions and manipulations. This buffer is used to block the
plates and dilute the standards, samples, detection antibodies and conjugates
in these E max ® ImmunoAssay Systems. The buffer is provided as 54ml of buffer
containing gentamicin as a preservative.
Note: The Block & Sample 5X Buffer should not be used with the TGFβ 1 E max ®
ImmunoAssay System.Storage Conditions: Store at 4°C.
TGFβ 1 E max ® ImmunoAssay Systems
Product Size Cat.# Price ($)
TGFβ 1 E max ® ImmunoAssay System 2 × 96 wells G7590 713.00
5 × 96 wells
For Research Use Only. Not for Use in Diagnostic Procedures.
G7591 1351.00
Description: The TGFβ 1 E max ® ImmunoAssay System provides optimized
reagents and a protocol for the sensitive and specific detection of transforming
growth factor β 1 (TGFβ 1). After an overnight coating of a 96-well plate, the
specific protein is detected using an antibody sandwich format. The system
uses horseradish peroxidase-conjugated secondary antibody and a singlecomponent
TMB substrate for the final chromogenic detection of bound TGFβ 1.
Using this system, biologically active TGFβ 1 in tissue culture supernatants,
plasma, serum or urine can be quantitated in the range of 15.6–1,000pg/ml.
Features:
• High Value: Optimized reagents and protocol provided.
• Specificity: Specific detection of TGFβ 1; less than 3% cross-reactivity
with other related growth factors (TGFβ 2 and TGFβ 3).
• Sensitivity: Detect picogram levels of factor per milliliter of sample.
• Flexibility: Available in sizes for two or five 96-well plates; can configure
plates as desired.
Storage Conditions: Store the entire system in its original package protected
from light at –20°C.
Protocol Part#
TGFß1 E ®
max ImmunoAssay System Technical Bulletin TB196
TGFβ Sample 10X Buffer
Product Size Cat.# Price ($)
TGFβ Sample 10X Buffer
For Research Use Only. Not for Use in Diagnostic Procedures.
20 ml G1291 78.00
Description: The TGFβ Sample 10X Buffer is an optimized proprietary buffer
designed for use with the TGFβ 1 E max ® ImmunoAssay System to reduce high
background, a common problem with traditional buffers used in TGFβ ELISAs.
Storage Conditions: Store at 4°C.
For complete and up-to-date product information visit: www.promega.com/catalog
Anti-pS 473 Akt pAb
Product Size Cat.# Price ($)
Anti-pS473 Akt pAb
For Research Use Only. Not for Use in Diagnostic Procedures.
40 µl G7441 500.00
Description: Anti-pS 473 Akt pAb is an affinity-purified polyclonal rabbit
antibody. The antibody is purified using a phosphorylated peptide that
corresponds to the phospho-S 473 form of Akt-1 and is useful for both Western
blotting and immunocytochemistry.
Features:
• Specificity: The antibody is selective for the Ser 473 phosphorylated
isoforms of Akt and does not show cross-reactivity with nonphosphorylated
Akt.
• Immunogen: Peptide from the singly phosphorylated Ser 473 region from
the C-terminus of Akt-1 protein.
• Antibody Form: Affinity-purified rabbit IgG, supplied in PBS with 50μg/ml
gentamicin.
• Value: Will generate 100ml of blotting solution, sufficient for 10 Western
blots of 10ml each.
Storage Conditions: Store at 4°C for daily/weekly use or dispense into
aliquots and store at –20°C for long-term storage.
Protocol Part#
Anti-pS473 Akt pAb Product Information 9PIG744
Immunocytochemical staining of Akt in embryonic rat brain cells.
Embryonic (day 17) rat brain cells were collected and treated with 20ng/ml
each of EGF and FGF. Anti-pS 473 Akt pAb was used at a 1:50 dilution. Positive
cells were visualized using a donkey anti-rabbit, Cy ® 3-conjugated secondary
antibody. Nuclei were stained using DAPI. Protocols developed and performed
at Promega.
See figure on next page for more information.
2769TA10_9A
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8
Imaging and Immunological Detection
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