2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
164<br />
HaloTag ® Fusion (N-Terminal) Mammalian<br />
Expression Vectors<br />
Product Size Cat.# Price ($)<br />
pHTN HaloTag ® CMV-neo Vector 20 µg G7721 355.00<br />
pFN28A HaloTag ® CMV-neo Flexi ® Vector 20 µg G8441 397.00<br />
pFN28K HaloTag ® CMV-neo Flexi ® Vector 20 µg G8451 397.00<br />
pFN21A HaloTag ® CMV Flexi ® Vector 20 µg G2821 397.00<br />
pFN21K HaloTag ® CMV Flexi ® Vector 20 µg G2831 397.00<br />
pFN22A HaloTag ® CMVd1 Flexi ® Vector 20 µg G2841 397.00<br />
pFN22K HaloTag ® CMVd1 Flexi ® Vector 20 µg G2851 397.00<br />
pFN23A HaloTag ® CMVd2 Flexi ® Vector 20 µg G2861 397.00<br />
pFN23K HaloTag ® CMVd2 Flexi ® Vector 20 µg G2871 397.00<br />
pFN24A HaloTag ® CMVd3 Flexi ® Vector 20 µg G2881 397.00<br />
pFN24K HaloTag ® CMVd3 Flexi ® Vector 20 µg G2981 397.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
For additional information see page 236.<br />
ELISAs and Antibodies<br />
BDNF E max ® ImmunoAssay Systems<br />
Product Size Cat.# Price ($)<br />
BDNF E max ® ImmunoAssay System 2 × 96 wells G7610 654.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
5 × 96 wells G7611 1245.00<br />
Description: The BDNF E max ® ImmunoAssay Systems provide optimized<br />
reagents and a protocol for the sensitive and specific detection of brainderived<br />
neurotrophic factor (BDNF). After an overnight coating of a 96-well<br />
plate, the specific protein is detected using an antibody sandwich format. The<br />
systems use a horseradish peroxidase-conjugated secondary antibody and a<br />
single-component TMB substrate for the final chromogenic detection of bound<br />
neurotrophic factor. Using this system, BDNF in tissue culture supernatants,<br />
tissue homogenates, plasma and urine can be quantitated in the range of<br />
7.8–500pg/ml. Binding and recovery from mouse brain homogenates has not<br />
been fully characterized.<br />
Features:<br />
• High Value: Optimized reagents and protocol provided.<br />
• Specificity: Specific detection of BDNF; less than 3% cross-reactivity with<br />
other related neurotrophic and growth factors.<br />
• Sensitivity: Detect picogram levels of factor per milliliter of sample.<br />
• Flexibility: Available in sizes for two or five 96-well plates; can configure<br />
plates as desired.<br />
Storage Conditions: Store the entire system in its original package protected<br />
from light at –20°C.<br />
Protocol Part#<br />
BDNF E max ® ImmunoAssay System Technical Bulletin TB257<br />
GDNF E max ® ImmunoAssay Systems<br />
Product Size Cat.# Price ($)<br />
GDNF E max ® ImmunoAssay System 2 × 96 wells G7620 654.00<br />
5 × 96 wells<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
G7621 1245.00<br />
Description: The GDNF E max ® ImmunoAssay Systems provide optimized<br />
reagents and a protocol for the sensitive and specific detection of glial cellline-derived<br />
neurotrophic factor (GDNF). After an overnight coating of a 96-well<br />
plate, the specific protein is detected using an antibody sandwich format. The<br />
systems use horseradish peroxidase-conjugated secondary antibody and a<br />
single-component TMB substrate for the final chromogenic detection of bound<br />
neurotrophic factor. Using this system, GDNF in tissue culture supernatants or<br />
tissue homogenates can be quantitated in the range of 15.6–1,000pg/ml.<br />
Features:<br />
• High Value: Optimized reagents and protocol provided.<br />
• Specificity: Specific detection of GDNF; less than 3% cross-reactivity with<br />
other related neurotrophic and growth factors.<br />
• Sensitivity: Detect picogram levels of factor per milliliter of sample.<br />
• Flexibility: Available in sizes for two or five 96-well plates; can configure<br />
plates as desired.<br />
Storage Conditions: Store the entire system in its original package protected<br />
from light at –20°C. Once thawed, store the system (except the GDNF<br />
Standard) at 4°C.<br />
Protocol Part#<br />
GDNF E max ® Immunoassay System Technical Bulletin TB221<br />
NGF E max ® ImmunoAssay Systems<br />
Product Size Cat.# Price ($)<br />
NGF E max ® ImmunoAssay System 2 × 96 wells G7630 654.00<br />
5 × 96 wells<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
G7631 1245.00<br />
Description: The NGF E max ® ImmunoAssay Systems provide optimized<br />
reagents and a protocol for the sensitive and specific detection of<br />
biologically active nerve growth factor (NGF). After an overnight coating of a<br />
96-well plate, the specific protein is detected using an antibody sandwich<br />
format. The systems use horseradish peroxidase-conjugated secondary<br />
antibody and a single-component TMB substrate for the final chromogenic<br />
detection of bound neurotrophic factor. The system can be used to<br />
quantitate NGF in tissue culture supernatants and tissue extracts in the range<br />
of 3.9–250pg/ml. Avoid using samples containing high levels of IgG such as<br />
serum, plasma and spleen.<br />
Features:<br />
• High Value: Optimized reagents and protocol provided.<br />
• Specificity: Specific detection of NGF; less than 3% cross-reactivity with<br />
other related neurotrophic and growth factors.<br />
• Sensitivity: Detect picogram levels of factor per milliliter of sample.<br />
• Flexibility: Available in sizes for two or five 96-well plates; can configure<br />
plates as desired.<br />
Storage Conditions: Store the entire system in its original package protected<br />
from light at –20°C.<br />
Protocol Part#<br />
NGF E max ® ImmunoAssay System Technical Bulletin TB226<br />
For complete and up-to-date product information visit: www.promega.com/catalog
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