2012 Promega catalogue

Cell Signaling
GenePrint ® Fluorescent STR Systems
Product Size Cat.# Price ($)
GammaSTR Multiplex (Fluorescein) 100 reactions DC6071 Pls. Enq.
D16S539, D7S820, D13S317, D5S818 400 reactions DC6070 Pls. Enq.
CSF1PO, TPOX, TH01, vWA Multiplex 100 reactions DC6301 Pls. Enq.
(Fluorescein)
Available Separately
400 reactions DC6300 Pls. Enq.
CTTv Allelic Ladder Mix (Fluorescein) 150 µl DG2121 Pls. Enq.
GammaSTR Allelic Ladder Mix (Fluorescein) 150 µl DG3291 Pls. Enq.
DG2121, DG3291 For Research Use Only. Not for Use in Diagnostic Procedures. DC6071,
DC6070, DC6301, DC6300 Not For Medical Diagnostic Use.
Description: The GenePrint ® Fluorescent STR Systems were developed for
use with the Hitachi FMBIO ® Fluorescence Imaging Systems and ABI PRISM ®
377 DNA Sequencer. One primer for each locus is labeled with fluorescein to
allow fluorescent detection. Fluorescein has an excitation maximum at 488nm
and an emission maximum at 532nm. Therefore, the systems are compatible
with a variety of fluorescence-detection instruments, including the ABI PRISM ®
310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130
and 3130xl Genetic Analyzers.
Each system provides all materials required to amplify STR regions of purified
genomic DNA except Taq DNA polymerase. Amplification of DNA using the
system components plus Taq DNA polymerase produces fluorescein-labeled
fragments representing alleles from the template DNA. For instruments that
support two-color fluorescence detection, additional precision may be achieved
by including the Internal Lane Standard 600 (Cat.# DG1071) in each gel lane
or injection.
Features:
• High-Throughput Analysis: Analysis is achieved by comparing amplified
DNA fragments directly with the allelic ladder provided for each locus.
• Efficiency: The fluorescent STR multiplex systems support simultaneous
single-tube amplification of four polymorphic STR loci with nonoverlapping
allele size ranges.
• Allelic Ladders: Comparing amplified alleles with allelic ladders provided
with each system allows rapid and reliable allele assignment.
Storage Conditions: Store at –20°C. The fluorescent primer pairs and allelic
ladders are light-sensitive; therefore, minimize light exposure.
Protocol Part#
GenePrint ® Fluorescent STR Systems Technical Manual TMD006
STR Systems
For complete and up-to-date product information visit: www.promega.com/catalog
GenePrint ® STR Systems (Silver Stain
Detection)
Product Size Cat.# Price ($)
GenePrint ® SilverSTR ® III System (D7S820, 100 reactions DC6451 Pls. Enq.
D13S317, D16S539)
400 reactions DC6450 Pls. Enq.
CSF1PO, TPOX, TH01 Multiplex 100 reactions DC6001 Pls. Enq.
400 reactions DC6000 Pls. Enq.
F13A01, FESFPS, vWA Multiplex 100 reactions DC6031 Pls. Enq.
Available Separately
400 reactions DC6030 Pls. Enq.
CTT Allelic Ladder Mix 150 µl DG2101 Pls. Enq.
FFv Allelic Ladder Mix 150 µl DG2141 Pls. Enq.
DG2101, DG2141 For Research Use Only. Not for Use in Diagnostic Procedures. DC6451,
DC6450, DC6001, DC6000, DC6031, DC6030 Not For Medical Diagnostic Use.
Description: The GenePrint ® Silver STR Systems provide a rapid, nonradioactive
method to evaluate small amounts (e.g., 1ng) of human DNA.
The systems provide all materials required to amplify STR regions of purified
genomic DNA except for Taq DNA polymerase and sample DNA. The amplified
STR fragments are separated by polyacrylamide gel electrophoresis and
detected by silver staining.
The combination of SilverSTR ® III, CTT and FFv provides access to seven of
the thirteen core loci that comprise the Combined DNA Index System (CODIS)
database.
Features:
• Economical: The GenePrint ® Silver STR Systems do not require
fluorescence-detection equipment for analysis. The systems are ideal for
labs that are starting STR analysis or do not wish to purchase expensive
fluorescence-detection equipment.
• Efficient: Analysis requires less than one day. Each multiplex allows
simultaneous amplification of three nonoverlapping STR loci for high
discrimination power.
Storage Conditions: Store at –20°C.
Protocol Part#
GenePrint ® STR Systems Technical Manual TMD004
Sex Identification Systems
GenePrint ® Sex Identification Systems
Product Size Cat.# Price ($)
Amelogenin (Fluorescein Detection) 100 reactions DC5171 Pls. Enq.
Amelogenin (TMR)
Not For Medical Diagnostic Use.
100 reactions DC6171 Pls. Enq.
Description: The GenePrint ® Sex Identification Systems can be used for sex
determination. When used under reaction conditions recommended in the
Technical Manuals (#TMD004, #TMD006 and #TMD008), a specific segment of
the human X chromosome generates a 212bp product, while the corresponding
human Y-chromosomal DNA segment produces a 218bp fragment. The Amelogenin
locus may be co-amplified and co-analyzed with a compatible multiplex
system by mixing the Amelogenin primers with those of the appropriate multiplex
system prior to use.
Storage Conditions: Store at –20°C. The fluorescent primer pair and ladder
are light-sensitive; therefore, minimize light exposure.
Protocol Part#
GenePrint ® Fluorescent STR Systems Technical Manual TMD006
PowerPlex ® 1.1 System Technical Manual TMD008
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7
Genetic Identity
Section
Contents
Table of
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