2012 Promega catalogue

Life
Science
Catalog
2012
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Table of
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Cell Signaling
Size Standards
158
PowerPlex ® 5-Dye Matrix Standards
Product Size Cat.# Price ($)
PowerPlex ® 5-Dye Matrix Standards, 310 50 µl DG4600 Pls. Enq.
PowerPlex ® 5-Dye Matrix Standards, 3100/3130 25 µl DG4700 Pls. Enq.
Not For Medical Diagnostic Use.
Description: The PowerPlex ® 5-Dye Matrix Standards allow the PowerPlex ®
ESX, ESI and 18D Systems to be analyzed on the ABI PRISM ® 310 Genetic
Analyzer (Cat.# DG4600) and the ABI PRISM ® 3100 and 3100-Avant or Applied
Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers (Cat.# DG4700).
Proper generation of a spectral calibration file is critical to evaluate multicolor
systems. The PowerPlex ® 5-Dye Matrix Standards contain matrix fragments
labeled with five fluorescent dyes: Fluorescein, JOE, TMR-ET, CXR-ET and
CC5. Once generated, the spectral calibration file is applied during collection
of PowerPlex ® data to calculate and compensate for spectral overlap between
different fluorescent dye colors.
Storage Conditions: Store at –20°C. The matrix standards are light-sensitive;
therefore, minimize light exposure.
Protocol Part#
PowerPlex ® 5-Dye Matrix Standards, 310 Technical Bulletin TBD023
PowerPlex ® 5-Dye Matrix Standards, 3100/3130 Technical Bulletin TBD024
PowerPlex ® 4-Dye Matrix Standards
Product Size Cat.# Price ($)
PowerPlex ® Matrix Standards, 310 50 µl DG4640 Pls. Enq.
PowerPlex ® Matrix Standards, 3100/3130
Not For Medical Diagnostic Use.
25 µl DG4650 Pls. Enq.
Description: The PowerPlex ® 4-Dye Matrix Standards allow the PowerPlex ®
16, PowerPlex ® 16 HS, PowerPlex ® ES, PowerPlex ® S5, PowerPlex ® Y,
PowerPlex ® CS7, Custom, and PowerPlex ® 16 and ES Monoplex Systems to
be analyzed on the ABI PRISM ® 310 Genetic Analyzer or ABI PRISM ® 377 DNA
Sequencer (Cat.# DG4640) and ABI PRISM ® 3100 and 3100-Avant or Applied
Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers (Cat.# DG4650).
Proper generation of a spectral calibration file is critical to evaluate multicolor
systems. The PowerPlex ® 4-Dye Matrix Standards contain matrix fragments
labeled with four fluorescent dyes: Fluorescein, JOE, TMR and CXR. Once
generated, the spectral calibration file is applied during collection of PowerPlex ®
data to calculate and compensate for spectral overlap between different
fluorescent dye colors.
Storage Conditions: Store at –20°C. The matrix standards are light-sensitive;
therefore, minimize light exposure.
Protocol Part#
PowerPlex ® Matrix Standards, 310 Technical Bulletin TBD021
PowerPlex ® Matrix Standards, 3100/3130 Technical Bulletin TBD022
PowerPlex ® Matrix Standards, 310/377
Product Size Cat.# Price ($)
PowerPlex ® Matrix Standards, 310/377
Not For Medical Diagnostic Use.
50 µl DG3640 155.00
Description: The PowerPlex ® Matrix Standards, 310/377, allow the
GenePrint ® Fluorescent STR Systems to be run on the ABI PRISM ® 310
Genetic Analyzer or ABI PRISM ® 377 DNA Sequencer.
Proper generation of a spectral calibration file is critical to evaluate multicolor
systems. The PowerPlex ® Matrix Standards, 310/377, contain matrix fragments
labeled with five fluorescent dyes: Fluorescein, JOE A, JOE B, TMR and CXR.
Once generated using the PowerPlex ® dye set, the spectral calibration file is
applied during the analysis of PowerPlex ® data to calculate and compensate
for the spectral overlap between different fluorescent dye colors. The Power-
Plex ® Matrix Standards, 310/377, contains two tubes of JOE, designated JOE A
and JOE B.
The PowerPlex ® Matrix Standards, 3100 (Cat.# DG3650 and X3121), have
been discontinued. Contact Promega Technical Services for more information.
Protocol Part#
PowerPlex ® Matrix Standards, 310/377 Technical Bulletin TBD018
Internal Lane Standard 600
Product Size Cat.# Price ($)
Internal Lane Standard 600
For Laboratory Use.
150 µl DG1071 354.00
Description: The Internal Lane Standard 600 (ILS 600) consists of 22 bands
ranging in size from 60bp to 600bp. Fragments of 60–200bp are spaced at
20bp intervals, fragments of 200–500bp are spaced every 25 bases, and fragments
of 500–600bp are spaced every 50 bases. Fragments that are multiples
of 100 bases have fluorescence intensities approximately twice that of other
fragments to simplify size assignment. The DNA ladder is double-stranded and
asymmetrically labeled with carboxy-X-rhodamine (CXR). The Internal Lane
Standard 600 is used to assign sizes to DNA fragments separated by electrophoresis
and detected using a variety of fluorescence-detection instruments
(e.g., Hitachi FMBIO ® Fluorescence Imaging System and ABI PRISM ® 310,
3100, 3100-Avant and Applied Biosystems 3130, 3130xl, 3500 and 3500xL
Genetic Analyzers). ILS 600 is commonly used as an internal size marker for
other applications and can be visualized by detecting fluorescent emission at
597nm after excitation at 576nm.
In addition, the Internal Lane Standard 600 contains additives that prevent the
formation of two artifacts (“split peak” and “n–10”) at the vWA locus in the
PowerPlex ® 16, 16 HS and PowerPlex ® ES Systems when using ABI PRISM ®
3100, 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers.
Storage Conditions: Store at –20°C. Avoid multiple freeze-thaw cycles or
exposure to frequent temperature changes. These fluctuations can greatly alter
product stability. The Internal Lane Standard 600 is light-sensitive; therefore,
minimize light exposure.
For complete and up-to-date product information visit: www.promega.com/catalog