2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 156 PowerPlex ® Y System Product Size Cat.# Price ($) PowerPlex ® Y System 50 reactions DC6761 Pls. Enq. 200 reactions DC6760 Pls. Enq. Available Separately Size Conc. Cat. # Price ($) Internal Lane Standard 600 150 µl DG1071 354.00 9948 Male DNA 250 ng 10 ng/µl DD2061 131.00 9947A DNA 250 ng 10 ng/µl DD1001 194.00 DG1071 For Laboratory Use. DC6761, DC6760, DD2061, DD1001 Not For Medical Diagnostic Use. Description: The PowerPlex ® Y System allows co-amplification and threecolor detection of twelve loci. The system contains primers for the loci DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. One primer specific for DYS389I/II, DYS391 and DYS439 is labeled with fluorescein (FL). One primer specific for each of the DYS385a/b, DYS390 and DYS393 loci is labeled with carboxy-tetramethylrhodamine (TMR). One primer specific for each of the DYS19, DYS392, DYS437 and DYS438 loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All twelve loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane. Fragment sizing is provided by an internal size standard (Internal Lane Standard 600) labeled with carboxy-X-rhodamine (CXR). Color deconvolution can be performed using matrix standards available from <strong>Promega</strong>. The PowerPlex ® Y System is compatible with the ABI PRISM ® 310, 3100 and 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers and ABI PRISM ® 377 DNA Sequencer. Features: • Amplification of 12 Y-STR Loci in One Reaction: Amplify the nine loci defined as the European minimal haplotype and endorsed by ISFG (International Society of Forensic Genetics)* plus the two loci added to this panel by SWGDAM (Scientific Working Group on DNA Analysis Methods). A. B. C. Note: The data in the PowerPlex ® Y Haplotype Database have been added to the US Y-STR Database, a searchable listing of 11- to 17-locus Y-STR haplotypes. For Y-STR haplotype searches, visit the US Y-STR Database at: www.usystrdatabase.org or Y-Chromosome Haplotype Reference Database at: www.yhrd.org *ISFG-endorsed loci are DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393. SWGDAM-endorsed loci are DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438 and DYS439. • Maximum Sensitivity: The system is optimized for use with 0.5–1ng of template DNA. However, each lot is performance tested to provide reproducible results with 0.25ng of DNA. Additional studies show interpretable results can be obtained with 100ng of female DNA. This makes the PowerPlex ® Y System a great choice to analyze “heavy” mixture samples. • Short PCR Products: Amplification products are all less than 335 bases, which increases the likelihood of obtaining a full profile from degraded samples. • No Locus Overlap: Primers were designed so that alleles for each locus do not overlap with a neighboring locus, reducing the likelihood of misinterpretation. • Typing of Samples with Greater Confidence: Allelic ladders for all 12 loci are provided to simplify interpretation through the use of the Power- Typer Macro. The wide span of alleles included in the ladder allows typing of more rare alleles. • Automatic Assignment of Genotypes: The PowerTyper Y Macro (available separately) automatically labels fragments from Genotyper ® data. The PowerTyper Macros can be downloaded or ordered on CD-ROM. Panels and bins text files for use with GeneMapper ® ID are available for download. Storage Conditions: Store at –20°C. The fluorescent primer pairs and allelic ladders are light-sensitive; therefore, minimize light exposure. Protocol Part# PowerPlex ® Y System Technical Manual TMD018 PowerPlex ® ES System The PowerPlex ® Y System. A single-source template DNA (0.5ng) was amplified using the PowerPlex ® Y 10X Primer Pair Mix. The amplification products were analyzed with an Applied Biosystems 3130 Genetic Analyzer using a 3kV, 5-second injection. The results were analyzed using GeneMapper ® ID software, version 3.2. Panel A. An electropherogram showing the peaks of the fluorescein-labeled loci: DYS389I/II, DYS391 and DYS439. Panel B. An electropherogram showing the peaks of the JOE-labeled loci: DYS19, DYS392, DYS437 and DYS438. Panel C. An electropherogram showing the peaks of the TMR-labeled loci: DYS385a/b, DYS390 and DYS393. For complete and up-to-date product information visit: www.promega.com/catalog 6043TA
Cell Signaling PowerPlex ® 16 and ES Monoplex Systems Product Size Cat.# Price ($) PowerPlex ® 16 Monoplex System, Penta E (Fluorescein) 100 reactions DC6591 706.00 PowerPlex ® 16 Monoplex System, Penta D (JOE) 100 reactions DC6651 706.00 PowerPlex ® ES Monoplex System, SE33 (JOE) 100 reactions DC6751 706.00 PowerPlex ® 16 Monoplex System D3S1358 (Fluorescein) 100 reactions DC6551 449.00 PowerPlex ® 16 Monoplex System TH01 (Fluorescein) 100 reactions DC6561 449.00 PowerPlex ® 16 Monoplex System D21S11 (Fluorescein) 100 reactions DC6571 449.00 PowerPlex ® 16 Monoplex System D18S51 (Fluorescein) 100 reactions DC6581 449.00 PowerPlex ® 16 Monoplex System D5S818 (JOE) 100 reactions DC6601 449.00 PowerPlex ® 16 Monoplex System D13S317 (JOE) 100 reactions DC6611 449.00 PowerPlex ® 16 Monoplex System D7S820 (JOE) 100 reactions DC6621 449.00 PowerPlex ® 16 Monoplex System D16S539 (JOE) 100 reactions DC6631 449.00 PowerPlex ® 16 Monoplex System CSF1PO (JOE) 100 reactions DC6641 449.00 PowerPlex ® 16 Monoplex System vWA (TMR) 100 reactions DC6661 449.00 PowerPlex ® 16 Monoplex System D8S1179 (TMR) 100 reactions DC6671 428.00 PowerPlex ® 16 Monoplex System TPOX (TMR) 100 reactions DC6681 449.00 PowerPlex ® 16 Monoplex System FGA (TMR) 100 reactions DC6691 449.00 Not For Medical Diagnostic Use. For complete and up-to-date product information visit: www.promega.com/catalog Description: The PowerPlex ® 16 and ES Monoplex Systems are available to amplify the Penta E, Penta D, SE33, D3S1358, TH01, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX or FGA locus. Each monoplex system allows amplification of a single locus to confirm results obtained with the PowerPlex ® 16 or PowerPlex ® ES System. The monoplex systems also can be used to re-amplify DNA samples when one or more of the loci do not amplify initially due to suboptimal amplification conditions or poor DNA quality. The PowerPlex ® 16 and PowerPlex ® ES Monoplex Systems contain primer pairs that have the same sequence as those used in the PowerPlex ® 16 HS (Cat.# DC2100, DC2101), PowerPlex ® 16 (Cat.# DC6530, DC6531), Power- Plex ® 16 BIO (Cat.# DC6540, DC6541) and PowerPlex ® ES Systems (Cat.# DC6730, DC6731). Allelic ladders are only provided in the following PowerPlex ® Monoplex Systems: DC6751, DC6591 and DC6651 [SE33 (JOE); Penta E (fluorescein) and Penta D (JOE), respectively]. Allelic ladders that are not provided are available by custom order. Please contact Technical Services for allelic ladder options based on the platform used. The PowerPlex ® 16 and ES Monoplex Systems were developed for use with the ABI PRISM ® 310, 3100 and 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers and are compatible with the Hitachi FMBIO ® II Fluorescence Imaging System. Storage Conditions: Store at –20°C. The fluorescent primer pair is lightsensitive; therefore, minimize light exposure. Protocol Part# TBD017 PowerPlex ® 16 and PowerPlex ® ES Monoplex Systems Technical Bulletin 157 7 Genetic Identity Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Page 111 and 112: Cell Signaling x-tracta Gel Extract
Page 113 and 114: Cell Signaling Wizard ® MagneSil
Page 115 and 116: Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118: Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120: Cell Signaling MagneSil ® KF, Geno
Page 121 and 122: Cell Signaling Plasmid Purification
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Page 125 and 126: Cell Signaling Wizard ® Plus Maxip
Page 127 and 128: Cell Signaling Wizard ® MagneSil
Page 129 and 130: Cell Signaling PureYield RNA Midipr
Page 131 and 132: Cell Signaling Maxwell ® 16 System
Page 133 and 134: Cell Signaling RNasin ® Plus RNase
Page 135 and 136: Cell Signaling DNA Quantitation Qua
Page 137 and 138: Cell Signaling Magnetic Stands and
Page 139 and 140: Cell Signaling 6 Drug Discovery For
Page 141 and 142: Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144: Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146: Cell Signaling Protease Assays Prot
Page 147 and 148: Cell Signaling Cell-Based Proteasom
Page 149 and 150: Cell Signaling Tryptase, Human, Rec
Page 151 and 152: Cell Signaling 7 Genetic Identity F
Page 153 and 154: Cell Signaling Maxwell ® 16 Forens
Page 155 and 156: Cell Signaling Plexor ® HY System
Page 157 and 158: Cell Signaling PowerPlex ® 18D Sys
Page 159: Cell Signaling PowerPlex ® CS7 Sys
Page 163 and 164: Cell Signaling GenePrint ® Fluores
Page 167 and 168: Cell Signaling HaloTag ® Ligand Bu
Page 169 and 170: Cell Signaling Block & Sample 5X Bu
Page 171 and 172: Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174: Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176: Cell Signaling Anti-Human GDNF pAb
Page 177 and 178: Cell Signaling Anti-PARP p85 Fragme
Page 179 and 180: Cell Signaling Anti-VAChT pAb Produ
Page 181 and 182: Cell Signaling VivoGlo Caspase 3/7
Page 185 and 186: Cell Signaling Microbial Detection
Page 187 and 188: 10 Instruments For complete and up-
Page 189 and 190: GloMax ® 20/20 Luminometer Product
Page 191 and 192: Fluorescence Module: Application-op
Page 193 and 194: GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196: Maxwell ® 16 Instruments Maxwell
Page 197 and 198: Maxwell ® 16 Flexi Method Firmware
Page 201 and 202: Cell Signaling Maxwell ® 16 System
Page 203 and 204: Cell Signaling Maxwell ® 16 Flexi
Page 205 and 206: Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR For complete
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
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Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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2012 Promega catalogue

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