2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
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Contents
Table of
Contents
Cell Signaling
156
PowerPlex ® Y System
Product Size Cat.# Price ($)
PowerPlex ® Y System 50 reactions DC6761 Pls. Enq.
200 reactions DC6760 Pls. Enq.
Available Separately Size Conc. Cat. # Price ($)
Internal Lane Standard 600 150 µl DG1071 354.00
9948 Male DNA 250 ng 10 ng/µl DD2061 131.00
9947A DNA 250 ng 10 ng/µl DD1001 194.00
DG1071 For Laboratory Use. DC6761, DC6760, DD2061, DD1001 Not For Medical Diagnostic
Use.
Description: The PowerPlex ® Y System allows co-amplification and threecolor
detection of twelve loci. The system contains primers for the loci DYS19,
DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437,
DYS438 and DYS439. One primer specific for DYS389I/II, DYS391 and DYS439
is labeled with fluorescein (FL). One primer specific for each of the DYS385a/b,
DYS390 and DYS393 loci is labeled with carboxy-tetramethylrhodamine (TMR).
One primer specific for each of the DYS19, DYS392, DYS437 and DYS438 loci
is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE).
All twelve loci are amplified simultaneously in a single tube and analyzed in
a single injection or gel lane. Fragment sizing is provided by an internal size
standard (Internal Lane Standard 600) labeled with carboxy-X-rhodamine
(CXR). Color deconvolution can be performed using matrix standards available
from Promega.
The PowerPlex ® Y System is compatible with the ABI PRISM ® 310, 3100 and
3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers and
ABI PRISM ® 377 DNA Sequencer.
Features:
• Amplification of 12 Y-STR Loci in One Reaction: Amplify the nine
loci defined as the European minimal haplotype and endorsed by ISFG
(International Society of Forensic Genetics)* plus the two loci added to this
panel by SWGDAM (Scientific Working Group on DNA Analysis Methods).
A.
B.
C.
Note: The data in the PowerPlex ® Y Haplotype Database have been added
to the US Y-STR Database, a searchable listing of 11- to 17-locus Y-STR
haplotypes. For Y-STR haplotype searches, visit the US Y-STR Database at:
www.usystrdatabase.org or Y-Chromosome Haplotype Reference Database
at: www.yhrd.org
*ISFG-endorsed loci are DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391,
DYS392, DYS393. SWGDAM-endorsed loci are DYS19, DYS385a/b, DYS389I/II,
DYS390, DYS391, DYS392, DYS393, DYS438 and DYS439.
• Maximum Sensitivity: The system is optimized for use with 0.5–1ng of
template DNA. However, each lot is performance tested to provide reproducible
results with 0.25ng of DNA. Additional studies show interpretable
results can be obtained with 100ng of female DNA. This
makes the PowerPlex ® Y System a great choice to analyze “heavy” mixture
samples.
• Short PCR Products: Amplification products are all less than 335 bases,
which increases the likelihood of obtaining a full profile from degraded
samples.
• No Locus Overlap: Primers were designed so that alleles for each locus
do not overlap with a neighboring locus, reducing the likelihood of
misinterpretation.
• Typing of Samples with Greater Confidence: Allelic ladders for all
12 loci are provided to simplify interpretation through the use of the Power-
Typer Macro. The wide span of alleles included in the ladder allows
typing of more rare alleles.
• Automatic Assignment of Genotypes: The PowerTyper Y Macro
(available separately) automatically labels fragments from Genotyper ® data.
The PowerTyper Macros can be downloaded or ordered on CD-ROM.
Panels and bins text files for use with GeneMapper ® ID are available for
download.
Storage Conditions: Store at –20°C. The fluorescent primer pairs and allelic
ladders are light-sensitive; therefore, minimize light exposure.
Protocol Part#
PowerPlex ® Y System Technical Manual TMD018
PowerPlex ® ES System
The PowerPlex ® Y System. A single-source template DNA (0.5ng) was amplified using the PowerPlex ® Y 10X Primer Pair Mix. The amplification
products were analyzed with an Applied Biosystems 3130 Genetic Analyzer using a 3kV, 5-second injection. The results were analyzed using GeneMapper ®
ID software, version 3.2. Panel A. An electropherogram showing the peaks of the fluorescein-labeled loci: DYS389I/II, DYS391 and DYS439. Panel B. An
electropherogram showing the peaks of the JOE-labeled loci: DYS19, DYS392, DYS437 and DYS438. Panel C. An electropherogram showing the peaks of the
TMR-labeled loci: DYS385a/b, DYS390 and DYS393.
For complete and up-to-date product information visit: www.promega.com/catalog
6043TA