2012 Promega catalogue

Cell Signaling
PowerPlex ® CS7 System, Custom
Product Size Cat.# Price ($)
PowerPlex ® CS7 System, Custom 100 reactions X6613 Pls. Enq.
Available Separately Size Conc. Cat. # Price ($)
Internal Lane Standard 600 150 µl DG1071 354.00
9947A DNA 250 ng 10 ng/µl DD1001 194.00
Water, Amplification Grade 6,250 µl DW0991 Pls. Enq.
DG1071, DW0991 For Laboratory Use. X6613, DD1001 Not For Medical Diagnostic Use.
Description: The PowerPlex ® CS7 System, Custom, is a multiplex STR assay
for relationship testing and human identification. The PowerPlex ® CS7 System,
Custom, allows co-amplification and three-color detection of seven STR loci,
including LPL, F13B, FESFPS, F13A01, Penta D, Penta C and Penta E. All seven
loci are amplified simultaneously in a single tube and analyzed in a single
injection. The PowerPlex ® CS7 System, Custom, contains two loci, Penta D
and Penta E, that overlap with the loci included in the PowerPlex ® 16 and
PowerPlex ® 16 HS Systems. This feature allows the PowerPlex ® CS7 System,
Custom, to be used as a confirmatory kit in paternity applications using the
five unshared STR loci to supplement the genotype and increase the available
information. The PowerPlex ® CS7 System, Custom, is compatible with the
ABI PRISM ® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems
3130 and 3130xl Genetic Analyzers. The PowerPlex ® CS7 System, Custom,
provides all materials necessary to amplify STR regions of purified genomic DNA.
Features:
• More Loci: Supplement current testing with LPL, F13B, FESFPS, F13A01
and Penta C for greater discrimination.
• Confirm Results: Overlap of Penta D and Penta E in the PowerPlex ® CS7
and 16 HS Systems allow detection of sample mixup when used together.
• Complete: Hot-start Taq DNA Polymerase is provided in the master mix,
and size standard is included.
Storage Conditions: Store at –20°C.
Protocol Part#
PowerPlex ® CS7 System Custom Technical Manual TMD030
For complete and up-to-date product information visit: www.promega.com/catalog
PowerPlex ® S5 System
Product Size Cat.# Price ($)
PowerPlex ® S5 System 100 reactions DC6951 Pls. Enq.
400 reactions DC6950 Pls. Enq.
Available Separately Size Conc. Cat. # Price ($)
Internal Lane Standard 600 150 µl DG1071 354.00
9947A DNA 250 ng 10 ng/µl DD1001 194.00
Water, Amplification Grade 6,250 µl DW0991 Pls. Enq.
DG1071, DW0991 For Laboratory Use. DC6951, DC6950, DD1001 Not For Medical Diagnostic
Use.
Description: The PowerPlex ® S5 System is a mini-STR kit that allows
co-amplification and detection of four STR markers (D18S51, D8S1179, TH01
and FGA) plus Amelogenin. One primer specific for each of the Amelogenin,
D18S51 and D8S1179 loci is labeled with fluorescein (FL), and one primer
specific for each of the TH01 and FGA loci is labeled with 6-carboxy-4´,5´dichloro-2´,7´-dimethoxy-fluorescein
(JOE). All five loci are amplified simultaneously
in a single tube and analyzed in a single injection. The four STR loci are
included in the CODIS and European databases. The amplicons for all loci are
smaller than 260bp. The PowerPlex ® S5 System was the first Promega STR kit
to include hot-start Taq DNA polymerase, which is included in the PowerPlex ®
S5 5X Master Mix. The PowerPlex ® S5 System is primarily a screening tool but
also can be used as a mini-STR casework kit.
Features:
• Sensitive: Generate full DNA profiles with as little as 50pg of DNA.
• Easy to Use: The PowerPlex ® S5 System comes complete with premixed
primer pairs, a master mix with Taq DNA polymerase and internal lane
standard. The simplified thermal cycling protocol requires no ramping, and
the system is compatible with a number of instrument platforms, including
ABI PRISM ® 310, 3100 and 3100-Avant and Applied Biosystems 3130
and 3130xl Genetic Analyzers.
• Robust: The PowerPlex ® S5 System is more tolerant of DNA degradation
and less sensitive to inhibitors. Full DNA profiles can be achieved in the
presence of 130μM hematin, 200ng tannic acid or 150ng humic acid.
• Automatic Assignment of Genotypes Available: Panels and bins text
files are required for use with GeneMapper ® ID software and are available
for download. The PowerTyper S5 Macro (available separately) facilitates
data analysis, allowing automatic assignment of genotypes using the Genotyper
® software. The PowerTyper Macros can be downloaded or ordered
on CD-ROM.
Storage Conditions: Store at –20°C.
Protocol Part#
PowerPlex ® S5 System Technical Manual TMD021
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7
Genetic Identity
Section
Contents
Table of
Contents