2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
154
PowerPlex ® 16 HS System
Product Size Cat.# Price ($)
PowerPlex ® 16 HS System 100 reactions DC2101 Pls. Enq.
400 reactions DC2100 Pls. Enq.
Available Separately Size Conc. Cat. # Price ($)
Internal Lane Standard 600 150 µl DG1071 354.00
9947A DNA 250 ng 10 ng/µl DD1001 194.00
Water, Amplification Grade 6,250 µl DW0991 Pls. Enq.
DG1071, DW0991 For Laboratory Use. DC2101, DC2100, DD1001 Not For Medical Diagnostic
Use.
Description: The PowerPlex ® 16 HS System is a multiplex STR system for
use in DNA typing. This system co-amplifies the loci D18S51, D21S11, TH01,
D3S1358, Penta E (labeled with fluorescein); FGA, TPOX, D8S1179, vWA and
Amelogenin (labeled with TMR); and CSF1PO, D16S539, D7S820, D13S317,
D5S818 and Penta D (labeled with JOE). This multiplex includes all 13 CODIS
STR markers, Amelogenin for gender determination and two low-stutter, highly
discriminating pentanucleotide STR markers. All sixteen loci are amplified simultaneously
in a single tube and analyzed in a single injection. The PowerPlex ®
16 HS System is compatible with ABI PRISM ® 310, 3100 and 3100-Avant
Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL
Genetic Analyzers.
Features:
• Robustness: The PowerPlex ® 16 HS System is more tolerant of PCR
inhibitors than competing STR systems and the previous version of
PowerPlex ® 16. Generate profiles with samples that previously failed to
amplify. Avoid costly and time-consuming sample cleanup.
• Sensitivity: Each lot is quality tested to produce full profiles from 100pg
of DNA. Gain confidence in analysis of limited samples.
• High Discrimination: The loci included in the PowerPlex ® 16 HS System
are more discriminating than competitive systems and are ideal for resolving
partial matches or challenging familial cases.
• Proven Design: Primer sequences, dyes and ladders are all unchanged
from PowerPlex ® 16. Expect concordance with existing databases.
• Complete System: The PowerPlex ® 16 HS System includes size standard,
amplification-grade water and Taq DNA polymerase in the master mix.
Simple to order, easy to use.
• Automatic Assignment of Genotypes: Panels and bins text files are
required for automatic assignment of genotypes using the GeneMapper ®
ID and ID-X software and are available for download.
Storage Conditions: Store at –20°C.
Protocol Part#
PowerPlex ® 16 HS System Technical Manual TMD022
PowerPlex ® 16 System
Product Size Cat.# Price ($)
PowerPlex ® 16 System 100 reactions DC6531 Pls. Enq.
400 reactions DC6530 Pls. Enq.
Available Separately Size Conc. Cat. # Price ($)
Internal Lane Standard 600 150 µl DG1071 354.00
9947A DNA 250 ng 10 ng/µl DD1001 194.00
DG1071 For Laboratory Use. DC6531, DC6530, DD1001 Not For Medical Diagnostic Use.
Description: The PowerPlex ® 16 System is a multiplex STR system for use in
DNA typing, including paternity testing, forensic DNA analysis, human identity
testing and tissue culture strain identification. The system allows co-amplification
and three-color detection of sixteen loci (fifteen STR loci and Amelogenin):
Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin,
Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One
primer for each of the Penta E, D18S51, D21S11, TH01 and D3S1358 loci is
labeled with fluorescein (FL); one primer for each of the FGA, TPOX, D8S1179,
vWA and Amelogenin loci is labeled with carboxy-tetramethylrhodamine
(TMR); and one primer for each of the Penta D, CSF1PO, D16S539, D7S820,
D13S317 and D5S818 loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´dimethoxy-fluorescein
(JOE). All sixteen loci are amplified simultaneously in a
single tube and analyzed in a single injection or gel lane.
The PowerPlex ® 16 System is compatible with the ABI PRISM ® 310, 3100 and
3100-Avant Genetic Analyzers, Applied Biosystems 3130 and 3130xl Genetic
Analyzers and ABI PRISM ® 377 DNA Sequencer.
Features:
• Single Amplification: Amplify all 13 CODIS STR loci in one reaction.
• Maximum Sensitivity: Analyze minute quantities of DNA. The system
is optimized for use with 1ng of DNA; however, each lot is performance
tested to provide reproducible results with 0.5ng of DNA. Additional studies
show interpretable results can be obtained with