2012 Promega catalogue

Cell Signaling
PowerPlex ® 18D System
Product Size Cat.# Price ($)
PowerPlex ® 18D System 200 reactions DC1802 Pls. Enq.
800 reactions DC1808 Pls. Enq.
Available Separately Size Conc. Cat. # Price ($)
CC5 Internal Lane Standard 500 300 µl DG1521 Pls. Enq.
Water, Amplification Grade 6,250 µl DW0991 Pls. Enq.
2800M Control DNA 25 µl 10 ng/µl DD7101 130.00
DG1521, DW0991 For Laboratory Use. DC1802, DC1808, DD7101 Not For Medical Diagnostic
Use.
Description: The PowerPlex ® 18D System is a multiplex STR system for use in
database and paternity testing. This system is optimized for direct amplification
of sample on FTA ® cards. This five-color multiplex allows co-amplification of
the 13 CODIS loci (D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179,
vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818) plus Amelogenin,
Penta E, Penta D, D2S1338 and D19S433. All eighteen loci are amplified simultaneously
in a single tube and analyzed in a single injection. The PowerPlex ®
18D System is compatible with ABI PRISM ® 3100 and 3100-Avant Genetic
Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic
Analyzers.
The PowerPlex 18D System was given NDIS approval in July 2011 for NDIS
CODIS databasing.
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For complete and up-to-date product information visit: www.promega.com/catalog
Features:
• Eliminate DNA Extraction: Simplify and shorten sample processing with
direct amplification from FTA ® cards.
• Reduce PCR Time: Amplify in less than 1.5 hours using rapid cycling
technology.
• Upload More Markers: Type D2S1338, D19S433, Penta D, Penta E,
Amelogenin and the 13 CODIS loci with one kit.
• Automatically Assign Genotypes: Panels and bins text files are required
for automatic assignment of genotypes using the GeneMapper ® ID and
ID-X software and are available for download.
Storage Conditions: Store kit at –20°C. Upon receipt, remove 2800M Control
DNA and store at 4°C.
Protocol Part#
PowerPlex ® 18D System Technical Manual TMD031
Configuration of the PowerPlex ® 18D System. The PowerPlex ® 18D
System contains all 13 CODIS loci: D3S1358, TH01, D21S11, D18S51,
D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX and
FGA, plus Amelogenin, Penta E, Penta D, D19S433 and D2S1338.
Amplification of sample using the PowerPlex ® 18D System. Two 1.2mm punches were taken from a buccal sample transferred to an FTA ®
card and amplified for 27 cycles using the PowerPlex ® 18D System. Amplification products were mixed with CC5 Internal Lane Standard 500 and
analyzed with an Applied Biosystems 3130xl Genetic Analyzer using a 3kV, 5-second injection. Results were analyzed using GeneMapper ® ID software,
version 3.2.
9654TA
9682TA
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Genetic Identity
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