2012 Promega catalogue

Cell Signaling
Plexor ® HY System
Product Size Cat.# Price ($)
Plexor ® HY System 200 reactions DC1001 Pls. Enq.
800 reactions DC1000 Pls. Enq.
Available Separately
Plexor ® Calibration Kit, Set A 1 each DC1500 Pls. Enq.
Water, Amplification Grade 6,250 µl DW0991 Pls. Enq.
DW0991 For Laboratory Use. DC1001, DC1500, DC1000 Not For Medical Diagnostic Use.
Description: The Plexor ® HY System is a real-time PCR assay to determine
the concentration of total human DNA and male human DNA simultaneously
in one reaction. The kit contains an internal PCR control (IPC) to test for falsenegative
results that may occur in the presence of PCR inhibitors and a melt
curve function to confirm that the correct product was amplified.
Plexor ® HY is a sensitive multiplex kit that routinely detects approximately 6.4pg
of total DNA. PCR setup is performed at room temperature and is compatible
with automated platforms.
The Plexor ® Systems work by measuring a reduction in fluorescent signal
during amplification. Amplification of each target uses only two primers, one
of which contains both a fluorescent tag and a modified base. As amplification
proceeds, fluorescence is reduced by site-specific incorporation of a fluorescent
quencher opposite the complementary modified base. The quencher is in close
proximity to a fluorescent dye located on the end of the primer, resulting in a
reduction of fluorescent signal. After PCR, a melt analysis can be performed to
provide an internal control for the final assay design or to expedite troubleshooting.
The Plexor ® HY System is optimized for use on the Applied Biosystems 7500
and 7500 FAST real-time PCR systems and Stratagene Mx3005P ® and
Mx3000P ® qPCR systems. For information about use with other qPCR instrumentation,
contact Promega Technical Services.
The Plexor ® Analysis Software is available for free download. The unique
functions of this software allow you to quickly and easily review data and create
reports. Replicate samples are automatically averaged, template amounts are
calculated and the necessary volume of DNA is displayed for your optimized
STR amplification conditions.
For complete and up-to-date product information visit: www.promega.com/catalog
Features:
• Simultaneous Quantification of Autosomal and Y-Chromosome
DNA: Less variability, less time, more valuable data.
• Consistent and Reproducible Detection of 6.4pg of DNA: If you can’t
detect it with Plexor ® HY, you can’t detect it with your STR system.
• Internal Positive Control and Melt-Curve Analysis: Guard against
false-negative and false-positive results, allowing you to be confident in
your data.
Storage Conditions: Store at –20°C.
Protocol Part#
Plexor ® HY System for the Applied Biosystems 7500 and 7500 FAST TM293
Real-Time PCR Systems Technical Manual
Plexor ® HY System for the Stratagene Mx3000P ® and Mx3005P TM294
Quantitative PCR Systems Technical Manual
Plexor ® HY System for the Bio-Rad iQ5 Real-Time PCR Detection TM296
System Technical Manual
Plexor ® HY System for the Corbett Rotor-Gene 6000 Series TM299
Detection System Technical Manual
Plexor ® HY System for the Roche LightCycler ® 480 Technical TM302
Manual
Fluorescent
Reporter
iso-dGTP
Autosomal amplification curves and melt curves from a Plexor ® HY amplification.
Dabcyl
iso-dC
Taq
Taq
Primer Annealing
and Extension
Incorporation of
Dabcyl-iso-dGTP
Fluorescence
Quenching
Schematic diagram illustrating the Plexor ® real-time PCR process.
6826TB
4909MA
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Genetic Identity
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