2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Sample Preparation
148
DNA IQ System
Product Size Cat.# Price ($)
DNA IQ System 100 reactions DC6701 536.00
400 reactions DC6700 1480.00
Casework Extraction Kit 100 reactions DC6745 Pls. Enq.
Tissue and Hair Extraction Kit
(for use with DNA IQ) 100 reactions DC6740 401.00
Available Separately
Lysis Buffer 150 ml A8261 Pls. Enq.
2X Wash Buffer 70 ml A8271 Pls. Enq.
Elution Buffer 50 ml A8281 Pls. Enq.
DNA IQ Resin 50 ml A8251 Pls. Enq.
DNA IQ Spin Baskets 1,000 /bag V1221 483.00
Microtubes, 1.5ml 1,000 /bag V1231 109.00
Proteinase K 100 mg V3021 156.00
DTT, Molecular Grade (Dry Powder) 5 g V3151 97.00
DC6701, DC6700, DC6740, V1231, V3021, V3151 For Research Use Only. Not for Use in
Diagnostic Procedures. A8261, A8271, A8281, A8251, V1221 Not For Medical Diagnostic Use.
Description:
The DNA IQ System is a DNA isolation system designed specifically for
forensic and paternity laboratories. This system employs novel paramagnetic
particles to isolate clean DNA for use with short tandem repeat (STR) analysis.
The DNA IQ System can be used to extract DNA from a variety of sample
types, including stains and liquid samples. Protocols for database samples
and casework samples are available.
The unique DNA IQ Resin removes PCR inhibitors and contaminants
frequently encountered in casework samples. When working with larger sample
volumes, such as those found in paternity and databasing, the DNA IQ
System can deliver a consistent amount of total DNA. Samples including buccal
swabs, liquid blood and stains on FTA ® and other blood cards have been used
with the DNA IQ System.
Some samples, such as tissue and hair, require pretreatment with proteinase K.
In addition, extracting DNA can be difficult from substrates such as denim,
envelopes, carpet, etc. The Casework Extraction Kit increases DNA yield from
challenging casework samples and substrates. The kit uses Proteinase K and
other reagents to remove proteins and other components from the DNA, helping
to ensure maximal yield and recovery of DNA from casework samples. The DNA
then can be purified using the DNA IQ System.
The DNA IQ System has been tested with the Plexor ® HY System and PowerPlex
® Systems to ensure a streamlined process. This translates into reliable
products that give optimal results from isolation to quantitation and STR analysis.
Genomic DNA isolation using the DNA IQ System has been automated on
the Biomek ® 2000 and 3000 laboratory automation workstations, as well as
the Tecan Freedom EVO ® liquid handler. Please contact Promega Technical
Services for additional information.
Features:
• Rapid: Only a few quick steps to obtain clean DNA with fewer PCR inhibitors.
• Flexible: One simple system for use with casework, paternity and database
samples.
• Efficient: Sensitive to minute sample sizes. In addition, no harmful organic
solvents such as phenol and chloroform are used, so use of a hood is not
required and disposal of hazardous chemicals is eliminated.
Storage Conditions:
Store the DNA IQ System at 22–25°C. Store the Tissue and Hair Extraction
Kit (for use with DNA IQ) at –20°C. Store the Casework Extraction Kit at 15–30°C.
Protocol Part#
DNA IQ System—Small Sample Casework Technical Bulletin TB296
DNA IQ System—Database Protocol Technical Bulletin TB297
DNA IQ Casework Pro Kit for Maxwell ® 16 Technical Manual TM332
Tissue and Hair Extraction Kit (for use with DNA IQ) Technical Bulletin TB307
Differex System
Product Size Cat.# Price ($)
Differex System 50 samples DC6801 488.00
200 samples DC6800 1659.00
Manual Differex Magnet
Available Separately
1 each V1591 183.00
Differex Digestion Buffer 150 ml A8501 Pls. Enq.
Differex Separation Solution 40 ml A8511 Pls. Enq.
V1591 For Research Use Only. Not for Use in Diagnostic Procedures. A8501, DC6801, A8511,
DC6800 Not For Medical Diagnostic Use.
Description: The Differex System extracts DNA from sexual assault
samples easily and quickly. It provides a simple and fast method for separating
male and female fractions of a sample, making it possible to analyze samples
more quickly and efficiently.
The Differex System offers recovery similar to that of the standard method
commonly used for differential extraction. The Differex System is used in
combination with the DNA IQ System and Slicprep 96 Device on robotic
platforms to process up to 48 differential extractions in less than 5 hours,
including incubation time, and less than 1 hour of hands-on time.
Automated Differex System methods are available for the Biomek ®
2000 and 3000 laboratory automation workstations, as well as the Tecan
Freedom EVO ® liquid handler. Please contact Promega Technical Services for
additional information. A manual protocol for the Differex System is available
for laboratories not yet using robotic platforms for DNA extraction.
Features:
• Automated Differential Extractions: The Differex System is the first
and only system that allows a forensic laboratory to automate every step of
differential extraction.
• Direct Compatibility with the DNA IQ System and Downstream
STR Applications: Clean DNA extracts mean you can be confident in your
ability to obtain results regardless of your choice of STR systems.
• Robust Results With Even Tough Samples: The Differex System
works with challenging new and old samples typical of those from sexual
assaults.
Storage Conditions: Store at room temperature.
Protocol Part#
Differex System Technical Bulletin TBD020
Differex System—For Use With the Differex Magnet Technical
Manual
TM331
Manual Differex Magnet.
For complete and up-to-date product information visit: www.promega.com/catalog
8650TA