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2012 Promega catalogue

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Cell Signaling<br />

Tris Base, Molecular Biology Grade<br />

Product Size Cat.# Price ($)<br />

Tris Base, Molecular Biology Grade 100 g H5133 36.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

500 g H5131 102.00<br />

2,500 g H5135 372.00<br />

Description: Tris Base, Molecular Biology Grade, is commonly used for many<br />

molecular biology applications.<br />

Formula: C 4H 11NO 3.<br />

Formula Weight: 121.14.<br />

Form: Crystalized free base.<br />

Properties:<br />

• pH at 25°C of 1M: 10.0–11.5.<br />

• Purity: ≥99.9%.<br />

• A 260 at 1M: ≤0.05.<br />

• A 280 at 1M: ≤0.05.<br />

• Melting Point: 167–172°C.<br />

• Moisture: ≤0.2%.<br />

• Lead: ≤2ppm.<br />

• Magnesium: ≤1ppm.<br />

• Calcium: ≤1ppm.<br />

• Iron: ≤1ppm.<br />

Features:<br />

• Quality Tested: Each lot of Tris Base is tested and certified to be free of<br />

DNase, RNase and protease activity.<br />

Storage Conditions: Store at 22–25°C.<br />

Tris-HCl, Molecular Biology Grade (Tris-<br />

Hydrochloride)<br />

Product Size Cat.# Price ($)<br />

Tris-HCl, Molecular Biology Grade 100 g H5121 63.00<br />

500 g H5123 198.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

2,500 g H5125 737.00<br />

Description: Tris-HCl, Molecular Biology Grade, is sometimes used in<br />

combination with Tris base for preparation of Tris-HCl buffers.<br />

Formula Weight: 157.56.<br />

Properties:<br />

• pH at 25°C (0.1M): 4.2–5.0.<br />

• Purity: ≥99.0%.<br />

• A 240 at 1M: ≤0.06.<br />

• A 260, A 280, A 300, A 600 at 1M: ≤0.05.<br />

• Melting Point: 150–152°C.<br />

• Moisture: ≤0.5%.<br />

• Calcium: ≤5ppm.<br />

• Iron: ≤5ppm.<br />

• Lead: ≤1ppm.<br />

• Magnesium: ≤1ppm.<br />

• Manganese: ≤1ppm.<br />

• Copper: ≤1ppm.<br />

• Zinc: ≤1ppm.<br />

Features:<br />

• Quality Tested: Each lot of Tris-HCl is tested and certified to be free of<br />

DNase, RNase and protease activity.<br />

Storage Conditions: Store at 22–25°C.<br />

For complete and up-to-date product information visit: www.promega.com/catalog<br />

Triton ® X-100, Molecular Biology Grade<br />

Product Size Cat.# Price ($)<br />

Triton ® X-100, Molecular Biology Grade 100 ml H5142 52.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

500 ml H5141 185.00<br />

Description: Triton ® X-100, Molecular Biology Grade, is a widely used<br />

nonionic surfactant.<br />

Properties:<br />

• Moisture: ≤1.0%.<br />

• Lead: ≤5ppm.<br />

• Iron: ≤5ppm.<br />

• Density at 25°C: 1.0645–1.0655g/ml.<br />

Features:<br />

• Quality Tested: Each lot of Triton ® X-100 is tested and certified to be free<br />

of DNase, RNase and protease activity.<br />

Storage Conditions: Store at 22–25°C.<br />

Tween ® 20, Molecular Biology Grade<br />

Product Size Cat.# Price ($)<br />

Tween ® 20, Molecular Biology Grade 100 ml H5152 52.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

500 ml H5151 185.00<br />

Description: Tween ® 20, Molecular Biology Grade, is a nonionic detergent<br />

used for many different molecular biology applications.<br />

Properties:<br />

• Appearance: Clear, yellow, viscous liquid.<br />

• Hydroxyl Number: 96–108.<br />

• Lead: ≤10ppm.<br />

Features:<br />

• Quality Tested: Each lot of Tween ® 20 is tested and certified to be free of<br />

DNase, RNase and protease activity.<br />

Storage Conditions: Store at 22–25°C.<br />

Urea<br />

Product Size Cat.# Price ($)<br />

Urea 1 kg V3171 92.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

5 kg V3175 340.00<br />

Description: Urea is a protein denaturant. Urea is qualified for use as the<br />

denaturing component in polyacrylamide gels.<br />

Formula: (NH 2) 2CO.<br />

Formula Weight: 60.06.<br />

Form: Fine, white, free-flowing pastilles.<br />

Properties:<br />

• Purity: ≥99.0%.<br />

• Melting Point: 132–135°C.<br />

• A 280 at 8M in water: ≤0.10.<br />

• Chloride: ≤0.0005%.<br />

• Heavy Metals: ≤0.001%.<br />

• Iron: ≤0.001%.<br />

• Cyanate: none detected.<br />

Storage Conditions: Store at 22–25°C. Protect from moisture.<br />

11<br />

1<br />

Biochemicals and Labware<br />

Section<br />

Contents<br />

Table of<br />

Contents

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