2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 144 Calpain-Glo Protease Assay Product Size Cat.# Price ($) Calpain-Glo Protease Assay 10 ml G8501 490.00 For Research Use Only. Not for Use in Diagnostic Procedures. 50 ml G8502 1267.00 Description: The Calpain-Glo Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (μ) and 2 (m) activities. Calpains are a family of calcium-activated cysteine proteases involved in cleaving a wide variety of proteins. Calpains modulate the biological activities of their substrates via limited proteolysis. The Calpain-Glo Protease Assay provides a succinyl, proluminescent calpain substrate, Suc-LLVY-aminoluciferin, in a buffer system optimized for calpain and luciferase activities. The addition of the calpain reagent in an “add-mix-measure” format results in calpain cleavage of the substrate and rapid development of a “glow-type” luminescent signal produced by the luciferase reaction. The signal is proportional to the amount of calpain activity present. The assay is designed for use with purified enzyme preparations. Features: • Faster Results: The homogeneous, enzyme-coupled format is especially well suited for rapidly autolysed enzymes like calpain; maximum sensitivity is reached in as little as 10 minutes, while the enzyme is fully active. • Simple Protocol: The homogeneous “add-mix-measure” protocol makes the assay easy to automate. • Greater Sensitivity: The assay is up to 1,000 times more sensitive than competitive fluorometric assays. The luminescent assay avoids inherent fluorescent background signals, providing excellent signal-to-background readings. The assay is linear over 4 logs of calpain concentration. Storage Conditions: Store components at –20°C protected from light. Protocol Part# Calpain-Glo Protease Assay Technical Bulletin TB344 Calpain I or II H Suc-LLVY – N S N Suc-LLVY + H2N S N COOH Luciferase Light N N S COOH S Aminoluciferin ATP, Mg ++ , O 2 The proluminescent substrate containing the Suc-LLVY sequence recognized by calpain. Following calpain cleavage, the substrate for luciferase (aminoluciferin) is released, allowing the luciferase reaction to occur and producing light. 5105MA Signal-to-Noise Ratio 100,000 10,000 1,000 100 10 1 0 0.001 Suc-LLVY-aminoluciferin; 12 minutes Suc-LLVY-aminoluciferin; 30 minutes Suc-LLVY-aminoluciferin; 60 minutes FRET substrate; 30 minutes Suc-LLVY-AMC; 60 minutes 0.01 0.1 1 10 Calpain I (nM) 1,000 For complete and up-to-date product information visit: www.promega.com/catalog 100 Sensitivity of the Calpain-Glo Protease Assay compared to fluorescent assays. 5108MA
Cell Signaling Tryptase, Human, Recombinant, β Product Size Cat.# Price ($) rhSkin β Tryptase 100 µg G7061 801.00 rhLung β Tryptase 100 µg G5631 801.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Tryptase is the predominant protein in mast cell granules and cleaves proteins at arginine and lysine residues. Tryptase is stored and released from mast cell granules upon activation. Mast cells are found in many tissues but are present in greater numbers along epithelial linings of the body, such as the skin, respiratory tract and gastrointestinal tract, as well as the perivascular tissue surrounding blood vessels. They are involved in a variety of physiological and pathophysiological states, including immediate hypersensitivity, delayedtype hypersensitivity, cell growth regulation, defense against neoplasia, and pain and itch sensation. They have also been implicated in chronic inflammatory states and are involved in neuroimmune interactions. The availability of recombinant human tryptase will aid in research directed toward a more complete understanding of the biological role(s) of tryptase and mast cells and the identification of tryptase in vivo targets. Skin β I Tryptase, Human, Recombinant (rhSkin β Tryptase) and Lung β II Tryptase, Human, Recombinant (rhLung β Tryptase) are neutral serine proteases. The human β tryptase enzymes have been cloned and stably expressed in Pichia pastoris as fully active tetrameric enzymes and purified by affinity chromatography. The two enzymes differ in buffer formulation, enzyme concentration and glycosylation pattern. rhLung Tryptase is provided at a much higher concentration (2mg/ml) in minimal buffer without heparin for chromatographic studies and with glycosylation more closely resembling cadaveric enzyme as demonstrated by glycosidase digestion followed by Western analysis of the two recombinant enzymes and native lung tryptase. Specific Activity: Measured as the rate of hydrolysis of 0.4mM Nα CBZ-L-Lysine Thiobenzyl Ester as substrate coupled with Ellman’s Reagent (5,5´-Dithio-bis(5-Nitrobenzoic Acid)) in a final volume of 1ml, incubating for 1 minute at 25°C, and monitoring the absorbance change at 410nm. One unit is defined as 1.0 absorbance unit change per minute. • rhSkin β Tryptase: >1,000 units/mg protein. • rhLung β Tryptase: >1,200 units/mg protein. Concentration: • rhSkin β Tryptase: 200μg/ml. • rhLung β Tryptase: 2mg/ml. Features: • High Specific Activity: Specific activity is consistently 130–150% higher than native lung tryptase. • Consistent: Recombinant protein expression results in uniform enzyme from batch to batch. • Safe: Void of human pathogens associated with native cadaveric tryptase. • Pure: Skin β and Lung β Tryptase are free of other contaminating proteases, providing more active enzyme per milligram of protein and eliminating extraneous protein interactions observed with native tryptase. Storage Conditions: Store at –20°C. For complete and up-to-date product information visit: www.promega.com/catalog undigested digested MW Native rhSkin rhLung Native rhSkin rhLung MW —34kDa —29kDa Glycosidase digestion of human β tryptase with PNGase F yields single tryptase core protein. Western blot with Anti-Human Tryptase mAb (clone AA5, Cat.# G3361). ADME Assays For additional information see page 20. Apoptosis For additional information see page 25. Cell Viability Assays For additional information see page 34. Cytotoxicity Assays For additional information see page 38. Glutathione Measurement For additional information see page 44. Histone Deacetylase Assays For additional information see page 52. Nuclear Receptor Pathway Tools For additional information see page 271. Protein Kinases and Kinase Assays For additional information see page 64. 3521TA08_1A 145 6 Drug Discovery Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98: Cell Signaling Kinases T4 Polynucle
Page 99 and 100: Cell Signaling S1 Nuclease Product
Page 101 and 102: Cell Signaling Percent Inhibition o
Page 103 and 104: Cell Signaling Untagged Flexi ® Ma
Page 105 and 106: Cell Signaling pGEM ® -5Zf(+) Vect
Page 107 and 108: Cell Signaling pSP64 Poly(A) Vector
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Page 111 and 112: Cell Signaling x-tracta Gel Extract
Page 113 and 114: Cell Signaling Wizard ® MagneSil
Page 115 and 116: Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118: Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120: Cell Signaling MagneSil ® KF, Geno
Page 121 and 122: Cell Signaling Plasmid Purification
Page 123 and 124: Cell Signaling PureYield Plasmid Ma
Page 125 and 126: Cell Signaling Wizard ® Plus Maxip
Page 127 and 128: Cell Signaling Wizard ® MagneSil
Page 129 and 130: Cell Signaling PureYield RNA Midipr
Page 131 and 132: Cell Signaling Maxwell ® 16 System
Page 133 and 134: Cell Signaling RNasin ® Plus RNase
Page 135 and 136: Cell Signaling DNA Quantitation Qua
Page 137 and 138: Cell Signaling Magnetic Stands and
Page 139 and 140: Cell Signaling 6 Drug Discovery For
Page 141 and 142: Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144: Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146: Cell Signaling Protease Assays Prot
Page 147: Cell Signaling Cell-Based Proteasom
Page 151 and 152: Cell Signaling 7 Genetic Identity F
Page 153 and 154: Cell Signaling Maxwell ® 16 Forens
Page 155 and 156: Cell Signaling Plexor ® HY System
Page 157 and 158: Cell Signaling PowerPlex ® 18D Sys
Page 159 and 160: Cell Signaling PowerPlex ® CS7 Sys
Page 161 and 162: Cell Signaling PowerPlex ® 16 and
Page 163 and 164: Cell Signaling GenePrint ® Fluores
Page 167 and 168: Cell Signaling HaloTag ® Ligand Bu
Page 169 and 170: Cell Signaling Block & Sample 5X Bu
Page 171 and 172: Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174: Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176: Cell Signaling Anti-Human GDNF pAb
Page 177 and 178: Cell Signaling Anti-PARP p85 Fragme
Page 179 and 180: Cell Signaling Anti-VAChT pAb Produ
Page 181 and 182: Cell Signaling VivoGlo Caspase 3/7
Page 185 and 186: Cell Signaling Microbial Detection
Page 187 and 188: 10 Instruments For complete and up-
Page 189 and 190: GloMax ® 20/20 Luminometer Product
Page 191 and 192: Fluorescence Module: Application-op
Page 193 and 194: GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196: Maxwell ® 16 Instruments Maxwell
Page 197 and 198: Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
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Cell Signaling 12 PCR For complete
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Cell Signaling TaqBead Hot Start Po
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
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Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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2012 Promega catalogue

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